C57BL/6JCya-Zwintem1flox/Cya
Common Name:
Zwint-flox
Product ID:
S-CKO-11551
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Zwint-flox
Strain ID
CKOCMP-52696-Zwint-B6J-VA
Gene Name
Product ID
S-CKO-11551
Gene Alias
2010007E07Rik; 2600001N01Rik; D10Ertd749e; Zwint-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zwintem1flox/Cya mice (Catalog S-CKO-11551) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000105431
NCBI RefSeq
NM_025635
Target Region
Exon 2~7
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Zwint, also known as ZW10 interactor or Zeste White 10-interacting kinetochore protein, is an essential component of the centromere and the mitotic spindle checkpoint. It can recruit dynamic protein kinase and dynein to promote chromosome movement and regulate the spindle assembly checkpoint (SAC), playing a crucial role in cell cycle regulation and chromosome segregation [3,8].
Functional studies, including knockdown experiments in various cancer cell lines, have revealed its significant role in cancer progression. For example, in melanoma cells, Zwint knockdown suppressed cell proliferation and migration, and this was associated with decreased expression of c-Myc, MMP-2, Slug, mTOR, p-mTOR, p-p38 and fibronectin, and increased expression of E-cadherin and MMP-9. Overexpression of c-Myc rescued the effects of Zwint knockdown on melanoma cell proliferation and migration, suggesting Zwint may act as an oncogene in melanoma by regulating c-Myc expression [1]. In lung cancer cell lines, knockdown of Zwint reduced cell proliferation, migration, invasion, apoptosis, and colony formation, and also decreased tumor volume in a mice tumor model. Transcriptome sequencing indicated potential ZWINT-related pathways such as TNF, P53, and PI3K signal networks [2]. Similar results were found in cervical cancer, pancreatic cancer, hepatocellular carcinoma, glioblastoma, and colorectal cancer, where Zwint promoted cell proliferation, migration, invasion or spheroid formation, often through regulating related signaling pathways like p53/p21 [4,5,6,7,9].
In conclusion, Zwint is crucial for spindle assembly checkpoint function and chromosome segregation during cell division. Model-based research, especially knockdown experiments in cancer cell lines, has demonstrated its oncogenic role in multiple cancers, suggesting it could be a potential therapeutic target for these diseases.
References:
1. Mou, Kuanhou, Zhang, Jian, Mu, Xin, Liu, Wenli, Ge, Rui. 2021. Zwint facilitates melanoma progression by promoting c-Myc expression. In Experimental and therapeutic medicine, 22, 818. doi:10.3892/etm.2021.10250. https://pubmed.ncbi.nlm.nih.gov/34131441/
2. Peng, Fang, Li, Qiang, Niu, Shao-Qing, Chen, Ming, Bao, Yong. 2019. ZWINT is the next potential target for lung cancer therapy. In Journal of cancer research and clinical oncology, 145, 661-673. doi:10.1007/s00432-018-2823-1. https://pubmed.ncbi.nlm.nih.gov/30643969/
3. He, Yan, Li, Rui, Gu, Liming, Yu, Shun, Wang, Gefei. 2020. Anaphase-promoting complex/cyclosome-Cdc-20 promotes Zwint-1 degradation. In Cell biochemistry and function, 38, 451-459. doi:10.1002/cbf.3499. https://pubmed.ncbi.nlm.nih.gov/31945194/
4. Ma, Zhe, Cai, Yufei, Tian, Chenchen. . ZWINT promotes the proliferation, migration, and invasion of cervical cancer cells by regulating the p53/p21 signaling pathway. In The Chinese journal of physiology, 66, 372-378. doi:10.4103/cjop.CJOP-D-23-00001. https://pubmed.ncbi.nlm.nih.gov/37929349/
5. Chen, Peng, He, Zhiwei, Wang, Jie, Liu, Xinyuan, Jiang, Jianxin. 2021. Hypoxia-Induced ZWINT Mediates Pancreatic Cancer Proliferation by Interacting With p53/p21. In Frontiers in cell and developmental biology, 9, 682131. doi:10.3389/fcell.2021.682131. https://pubmed.ncbi.nlm.nih.gov/34900978/
6. Lin, Tong, Zhang, Yingzhao, Lin, Zhimei, Peng, Lisheng. 2021. ZWINT is a Promising Therapeutic Biomarker Associated with the Immune Microenvironment of Hepatocellular Carcinoma. In International journal of general medicine, 14, 7487-7501. doi:10.2147/IJGM.S340057. https://pubmed.ncbi.nlm.nih.gov/34744456/
7. Yang, Li, Han, Na, Zhang, Xiaoxi, Chen, Rui, Zhang, Mengxian. 2020. ZWINT: A potential therapeutic biomarker in patients with glioblastoma correlates with cell proliferation and invasion. In Oncology reports, 43, 1831-1844. doi:10.3892/or.2020.7573. https://pubmed.ncbi.nlm.nih.gov/32323832/
8. Woo Seo, Dong, Yeop You, Seung, Chung, Woo-Jae, Kim, Jae-Sung, Su Oh, Jeong. 2015. Zwint-1 is required for spindle assembly checkpoint function and kinetochore-microtubule attachment during oocyte meiosis. In Scientific reports, 5, 15431. doi:10.1038/srep15431. https://pubmed.ncbi.nlm.nih.gov/26486467/
9. Akabane, Shintaro, Oue, Naohide, Sekino, Yohei, Ohdan, Hideki, Yasui, Wataru. 2021. KIFC1 regulates ZWINT to promote tumor progression and spheroid formation in colorectal cancer. In Pathology international, 71, 441-452. doi:10.1111/pin.13098. https://pubmed.ncbi.nlm.nih.gov/33819373/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen