C57BL/6JCya-Ldhdem1flox/Cya
Common Name:
Ldhd-flox
Product ID:
S-CKO-11559
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ldhd-flox
Strain ID
CKOCMP-52815-Ldhd-B6J-VA
Gene Name
Product ID
S-CKO-11559
Gene Alias
4733401P21Rik; D8Bwg1320e; DLD
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ldhdem1flox/Cya mice (Catalog S-CKO-11559) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000070004
NCBI RefSeq
NM_027570
Target Region
Exon 1~8
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Ldhd, or lactate dehydrogenase D, is an enzyme involved in anaerobic glycolysis. It specifically catalyzes the oxidation of D-lactate to pyruvate. This process is part of the mitochondrial D-lactate catabolism pathway, which is important for maintaining normal cellular metabolism [1,4].
In esophageal squamous cell carcinoma (ESCC), the CDK7-YAP-Ldhd axis promotes D-lactate elimination and ferroptosis defense, supporting cancer stem cell-like properties. High Ldhd status is linked to poor prognosis in ESCC patients [1]. In hepatocellular carcinoma, knockdown of Ldhd in HepG2 and Huh7 cells promoted cell proliferation, migration, and invasion, while overexpression showed the opposite effects, indicating its role in cancer prognosis [3]. In addition, Ldhd mutations have been associated with hyperuricaemia and gout [2], D-lactic acidosis [4,5,6], and neurological manifestations [5].
In conclusion, Ldhd plays a crucial role in D-lactate metabolism and is involved in multiple disease conditions, such as various cancers, D-lactic acidosis, and related neurological problems. Studies on Ldhd, including those using gene-knockout models in the context of these diseases, have enhanced our understanding of its biological functions and its potential as a therapeutic target.
References：
1. Lv, Mengzhu, Gong, Ying, Liu, Xuesong, Zhang, Weimin, Zhan, Qimin. 2023. CDK7-YAP-LDHD axis promotes D-lactate elimination and ferroptosis defense to support cancer stem cell-like properties. In Signal transduction and targeted therapy, 8, 302. doi:10.1038/s41392-023-01555-9. https://pubmed.ncbi.nlm.nih.gov/37582812/
2. Onuora, Sarah. . LDHD mutation leads to hyperuricaemia and gout. In Nature reviews. Rheumatology, 16, 2. doi:10.1038/s41584-019-0347-y. https://pubmed.ncbi.nlm.nih.gov/31784723/
3. Wang, Shengnan, Wu, Xingwei, Wu, Xiaoming, Chen, Qianyi, Qi, Zhilin. 2024. Systematic analysis of the role of LDHs subtype in pan-cancer demonstrates the importance of LDHD in the prognosis of hepatocellular carcinoma patients. In BMC cancer, 24, 156. doi:10.1186/s12885-024-11920-8. https://pubmed.ncbi.nlm.nih.gov/38291366/
4. Jin, Shan, Chen, Xingchen, Yang, Jun, Ding, Jianping. 2023. Lactate dehydrogenase D is a general dehydrogenase for D-2-hydroxyacids and is associated with D-lactic acidosis. In Nature communications, 14, 6638. doi:10.1038/s41467-023-42456-3. https://pubmed.ncbi.nlm.nih.gov/37863926/
5. Kwong, Anna Ka-Yee, Wong, Sheila Suet-Na, Rodenburg, Richard J T, Chan, Godfrey Chi Fung, Fung, Cheuk-Wing. 2021. Human d-lactate dehydrogenase deficiency by LDHD mutation in a patient with neurological manifestations and mitochondrial complex IV deficiency. In JIMD reports, 60, 15-22. doi:10.1002/jmd2.12220. https://pubmed.ncbi.nlm.nih.gov/34258137/
6. Monroe, Glen R, van Eerde, Albertien M, Tessadori, Federico, van Haaften, Gijs, Jans, Judith J. 2019. Identification of human D lactate dehydrogenase deficiency. In Nature communications, 10, 1477. doi:10.1038/s41467-019-09458-6. https://pubmed.ncbi.nlm.nih.gov/30931947/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen