C57BL/6JCya-Ube2kem1flox/Cya
Common Name:
Ube2k-flox
Product ID:
S-CKO-11586
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ube2k-flox
Strain ID
CKOCMP-53323-Ube2k-B6J-VA
Gene Name
Product ID
S-CKO-11586
Gene Alias
D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ube2kem1flox/Cya mice (Catalog S-CKO-11586) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000142407
NCBI RefSeq
NM_016786
Target Region
Exon 4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Ube2k, a ubiquitin-conjugating enzyme, is involved in the ubiquitin-proteasome system, responsible for numerous cellular physiological activities. It participates in the synthesis of K48-linked ubiquitin chains, which target substrates for proteasomal degradation [5]. This process is crucial for maintaining normal cellular function, regulating protein levels, and controlling various biological pathways.
In the context of diseases, in hepatocellular carcinoma (HCC), Ube2k expression is elevated, promoting cell proliferation, migration, and stemness property, and a worse prognosis for patients. Hypoxia can induce HIF-1α-mediated increase of Ube2k levels in HCC cells, and Ube2k cooperates with HIF-1α to form a functional axis to drive HCC progression [2,3].
In pancreatic ductal adenocarcinoma (PDAC), Ube2k is highly expressed, promoting cell proliferation and the stemness phenotype both in vitro and in vivo, and IGF2BP3 increases Ube2k expression by enhancing its RNA stability [4].
In glioma cells, Ube2k is highly expressed, and silencing it inhibits cell growth by inducing autophagy-related apoptosis [8].
In multiple myeloma, ixazomib, a 20S proteasome inhibitor, can inhibit cell proliferation by targeting Ube2k [7].
In depression, microglial circ-UBE2K binds to HNRNPU to upregulate Ube2k expression, leading to abnormal microglial activation, neuroinflammation, and promoting the development of major depressive disorder (MDD) [1].
In Alzheimer's disease, elevated Ube2k induced synaptic dysfunction through ubiquitinating K1082 on GluN2B [6].
In conclusion, Ube2k plays a significant role in multiple biological processes and disease conditions. Its involvement in cancer progression, neurodegenerative diseases like Alzheimer's, and mental disorders such as depression indicates its potential as a therapeutic target. Functional studies, including those using gene knockout or conditional knockout mouse models (if applicable in the future), will further enhance our understanding of Ube2k's role in these diseases, providing new strategies for diagnosis and treatment.
References：
1. Cai, Yujie, Ji, Yao, Liu, Yingxuan, Wang, Yan, Cui, Lili. 2024. Microglial circ-UBE2K exacerbates depression by regulating parental gene UBE2K via targeting HNRNPU. In Theranostics, 14, 4058-4075. doi:10.7150/thno.96890. https://pubmed.ncbi.nlm.nih.gov/38994030/
2. Lei, Xiangxiang, Hu, Xiaoge, Lu, Qiliang, Huang, Dongsheng, Xu, Qiuran. 2022. UBE2K promotes the malignant progression of hepatocellular carcinoma by regulating c-Myc. In Biochemical and biophysical research communications, 638, 210-218. doi:10.1016/j.bbrc.2022.11.046. https://pubmed.ncbi.nlm.nih.gov/36481361/
3. Ouyang, Guangxiong, Fu, Wen, Guo, Jinhui, Huang, Dongsheng, Zhang, Yaping. 2023. Hypoxia-induced UBE2K promotes the malignant progression of HCC. In Pathology, research and practice, 245, 154422. doi:10.1016/j.prp.2023.154422. https://pubmed.ncbi.nlm.nih.gov/37003132/
4. Fu, Wen, Lei, Xiangxiang, Lu, Qiliang, Tong, Xiangmin, Hu, Xiaoge. 2023. UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinoma. In International journal of oncology, 62, . doi:10.3892/ijo.2023.5500. https://pubmed.ncbi.nlm.nih.gov/36896783/
5. Nakasone, Mark A, Majorek, Karolina A, Gabrielsen, Mads, Smith, Brian O, Huang, Danny T. 2022. Structure of UBE2K-Ub/E3/polyUb reveals mechanisms of K48-linked Ub chain extension. In Nature chemical biology, 18, 422-431. doi:10.1038/s41589-021-00952-x. https://pubmed.ncbi.nlm.nih.gov/35027744/
6. Wang, Xiong, Xie, Jiazhao, Tan, Lu, Li, Xiaoguang, Cheng, Liming. 2023. N6-methyladenosine-modified circRIMS2 mediates synaptic and memory impairments by activating GluN2B ubiquitination in Alzheimer's disease. In Translational neurodegeneration, 12, 53. doi:10.1186/s40035-023-00386-6. https://pubmed.ncbi.nlm.nih.gov/38012808/
7. Wang, Qingqing, Dong, Zhigao, Su, Junnan, Ma, Lili, Chen, Xuyan. 2021. Ixazomib inhibits myeloma cell proliferation by targeting UBE2K. In Biochemical and biophysical research communications, 549, 1-7. doi:10.1016/j.bbrc.2021.02.048. https://pubmed.ncbi.nlm.nih.gov/33647537/
8. Xin, Zhen, Holgersson, Kristian, Zhu, Pengcheng, Szekely, Laszlo, Wu, Tao. . Silencing UBE2K inhibits the growth of glioma cells by inducing the autophagy-related apoptosis. In Journal of biochemical and molecular toxicology, 38, e23758. doi:10.1002/jbt.23758. https://pubmed.ncbi.nlm.nih.gov/38963134/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen