C57BL/6JCya-Cpsf4em1flox/Cya
Common Name:
Cpsf4-flox
Product ID:
S-CKO-11703
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Cpsf4-flox
Strain ID
CKOCMP-54188-Cpsf4-B6J-VA
Gene Name
Product ID
S-CKO-11703
Gene Alias
30kDa
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cpsf4em1flox/Cya mice (Catalog S-CKO-11703) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000160422
NCBI RefSeq
NM_001374716
Target Region
Exon 4~5
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Cpsf4, short for Cleavage and polyadenylation specificity factor 4, is a crucial component of the pre-mRNA 3' end processing complex, which is involved in RNA alternative splicing (AS), an important stage in controlling gene expression [4].
In multiple cancer types, Cpsf4 has been shown to act as an oncogene. In lung cancer, it promotes the tumor-initiating phenotype and chemoresistance by enhancing VEGF/NRP2/TAZ signaling [1]. In hepatocellular carcinoma (HCC), Cpsf4 reduces circRNA levels, disrupts miRNA-mediated gene silencing, and promotes cell proliferation [2]. In triple-negative breast cancer, it promotes metastasis by upregulating MDM4 [3], and also regulates alternative splicing of HMG20B to facilitate cancer progression [6]. In oral squamous cell carcinoma, Cpsf4 promotes cell proliferation and invasion via the PI3K-AKT signaling pathway [5]. In prostate cancer, high Cpsf4 expression is associated with enhanced cell migration and cell cycle dysregulation [8]. In bladder cancer, knockdown of Cpsf4 inhibits cell growth by upregulating NRF1 [9]. In breast cancer, miR-4458 can inhibit cell growth, migration, and invasiveness by targeting Cpsf4 [10]. In lung adenocarcinomas, Cpsf4 activates telomerase reverse transcriptase and is associated with poor prognosis [7].
In conclusion, Cpsf4 is significantly involved in the regulation of multiple biological processes related to cancer development, such as cell proliferation, invasion, metastasis, and chemoresistance. Studies, including those using knockdown models (functionally similar to gene-knockout in revealing gene function), have shown its oncogenic role across various cancer types, highlighting its potential as a prognostic marker and therapeutic target in cancer research.
References:
1. Song, YingQiu, Sun, Kai, Gong, LiLan, Zheng, FeiMeng, Li, GuiLing. 2022. CPSF4 promotes tumor-initiating phenotype by enhancing VEGF/NRP2/TAZ signaling in lung cancer. In Medical oncology (Northwood, London, England), 40, 62. doi:10.1007/s12032-022-01919-1. https://pubmed.ncbi.nlm.nih.gov/36567417/
2. Wang, Xueying, Dong, Jiani, Li, Xiaojing, Cheng, Zeneng, Zhu, Qubo. 2021. CPSF4 regulates circRNA formation and microRNA mediated gene silencing in hepatocellular carcinoma. In Oncogene, 40, 4338-4351. doi:10.1038/s41388-021-01867-6. https://pubmed.ncbi.nlm.nih.gov/34103682/
3. Lee, Kaping, Zheng, Qiufan, Lu, Qianyi, Deng, Wuguo, Wang, Shusen. 2021. CPSF4 promotes triple negative breast cancer metastasis by upregulating MDM4. In Signal transduction and targeted therapy, 6, 184. doi:10.1038/s41392-021-00565-9. https://pubmed.ncbi.nlm.nih.gov/34006850/
4. Yuemaierabola, Anwaier, Guo, Jun, Sun, Lili, Guo, Wenjia, Cao, Yan. 2023. Comprehensive analysis of CPSF4-related alternative splice genes in hepatocellular carcinoma. In Journal of cancer research and clinical oncology, 149, 13955-13971. doi:10.1007/s00432-023-05178-z. https://pubmed.ncbi.nlm.nih.gov/37542549/
5. Zhang, Mingjie, Lin, Han, Ge, Xiaohan, Xu, Yue. 2021. Overproduced CPSF4 Promotes Cell Proliferation and Invasion via PI3K-AKT Signaling Pathway in Oral Squamous Cell Carcinoma. In Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 79, 1177.e1-1177.e14. doi:10.1016/j.joms.2020.12.047. https://pubmed.ncbi.nlm.nih.gov/33535057/
6. Zhong, Guansheng, Shen, Qinyan, Zheng, Xinli, Dai, Zhijun, Lou, Weiyang. 2024. CPSF4-mediated regulation of alternative splicing of HMG20B facilitates the progression of triple-negative breast cancer. In Journal of translational medicine, 22, 1149. doi:10.1186/s12967-024-06004-x. https://pubmed.ncbi.nlm.nih.gov/39731153/
7. Chen, Wangbing, Qin, Lijun, Wang, Shusen, Huang, Wenlin, Deng, Wuguo. 2014. CPSF4 activates telomerase reverse transcriptase and predicts poor prognosis in human lung adenocarcinomas. In Molecular oncology, 8, 704-16. doi:10.1016/j.molonc.2014.02.001. https://pubmed.ncbi.nlm.nih.gov/24618080/
8. Choudhry, Muhammad, Gamallat, Yaser, Ghosh, Sunita, Bismar, Tarek A. 2023. Cleavage and Polyadenylation-Specific Factor 4 (CPSF4) Expression Is Associated with Enhanced Prostate Cancer Cell Migration and Cell Cycle Dysregulation, In Vitro. In International journal of molecular sciences, 24, . doi:10.3390/ijms241612961. https://pubmed.ncbi.nlm.nih.gov/37629142/
9. Sun, Yixiang, Li, Guanglei, Zhang, Hanlin, Xie, Mao. 2024. Knockdown of CPSF4 Inhibits Bladder Cancer Cell Growth by Upregulating NRF1. In Biochemical genetics, , . doi:10.1007/s10528-024-10891-6. https://pubmed.ncbi.nlm.nih.gov/39039322/
10. Wu, Jianrong, Miao, Juan, Ding, Ye, Zhou, Xue, Tang, Ranran. 2019. MiR-4458 inhibits breast cancer cell growth, migration, and invasiveness by targeting CPSF4. In Biochemistry and cell biology = Biochimie et biologie cellulaire, 97, 722-730. doi:10.1139/bcb-2019-0008. https://pubmed.ncbi.nlm.nih.gov/30970220/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen