C57BL/6JCya-Sult2b1em1flox/Cya
Common Name:
Sult2b1-flox
Product ID:
S-CKO-11711
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Sult2b1-flox
Strain ID
CKOCMP-54200-Sult2b1-B6J-VA
Gene Name
Product ID
S-CKO-11711
Gene Alias
ST2B1; SULT2B
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sult2b1em1flox/Cya mice (Catalog S-CKO-11711) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000075571
NCBI RefSeq
NM_017465
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Sult2b1, a member of the sulfotransferase family, is responsible for expressing SULT2B1a and SULT2B1b enzymes, which are phase II metabolizing enzymes involved in steroids and hydroxysteroids homeostasis [6]. SULT2B1 is the key enzyme that catalyzes the synthesis of cholesterol sulfate [3]. It is involved in multiple biological pathways, such as lipid metabolism, immune cell regulation, and cell proliferation and migration [1,2,5]. Genetic models, like knockout mice, have been crucial in studying its functions.
In Sult2b1 -/- mice, larger infarction and worse neurological scores were observed after transient middle cerebral artery occlusion, with peripheral monocyte-derived macrophages promoting a pro-inflammatory status [3]. In age-related macular degeneration, Sutl2b1 deficiency reduced leakage areas and inhibited pathological angiogenesis by inhibiting M2 macrophage activation [4]. In colorectal cancer, SULT2B1 knockout suppressed cell proliferation and migration, indicating its oncogenic role [5]. In atherosclerotic mice, knockdown of Sult2b1 led to remission of AS and reduced inflammation levels [7].
In conclusion, Sult2b1 plays essential roles in various biological processes including inflammation, lipid metabolism, and cell proliferation. The use of Sult2b1 knockout mouse models has significantly contributed to understanding its functions in diseases such as ischemic stroke, age-related macular degeneration, colorectal cancer, and atherosclerosis. These findings provide potential therapeutic targets for these diseases.
References:
1. Che, Gang, Wang, Wankun, Wang, Jiawei, Yang, Yan, Liu, Jian. . Sulfotransferase SULT2B1 facilitates colon cancer metastasis by promoting SCD1-mediated lipid metabolism. In Clinical and translational medicine, 14, e1587. doi:10.1002/ctm2.1587. https://pubmed.ncbi.nlm.nih.gov/38372484/
2. Wang, Shuai, Wang, Rui, Xu, Nan, Lu, Di, Xu, Xiao. 2023. SULT2B1-CS-DOCK2 axis regulates effector T-cell exhaustion in HCC microenvironment. In Hepatology (Baltimore, Md.), 78, 1064-1078. doi:10.1097/HEP.0000000000000025. https://pubmed.ncbi.nlm.nih.gov/36626623/
3. Wang, Yan, Jin, Haojie, Wang, Yafang, Zhao, Heng, Wang, Feng. 2021. Sult2b1 deficiency exacerbates ischemic stroke by promoting pro-inflammatory macrophage polarization in mice. In Theranostics, 11, 10074-10090. doi:10.7150/thno.61646. https://pubmed.ncbi.nlm.nih.gov/34815805/
4. Wang, Yafang, Liu, Yang, Wang, Yan, Wang, Fenghua, Sun, Xiaodong. 2023. Macrophage Sult2b1 promotes pathological neovascularization in age-related macular degeneration. In Life science alliance, 6, . doi:10.26508/lsa.202302020. https://pubmed.ncbi.nlm.nih.gov/37550000/
5. Ma, Jianxing, Sun, Fengyao, Li, Wen, Yan, Siyuan, Wang, Chen. 2024. SULT2B1: a novel therapeutic target in colorectal cancer via modulation of AKT/PKM2-mediated glycolysis and proliferation. In Journal of translational medicine, 22, 1093. doi:10.1186/s12967-024-05910-4. https://pubmed.ncbi.nlm.nih.gov/39623433/
6. Alherz, Fatemah A. 2024. Human sulfotransferase SULT2B1 physiological role and the impact of genetic polymorphism on enzyme activity and pathological conditions. In Frontiers in genetics, 15, 1464243. doi:10.3389/fgene.2024.1464243. https://pubmed.ncbi.nlm.nih.gov/39280099/
7. Pan, Hangyu, Wu, Tongwei, Huang, Kang, Yin, Mengzhuo, Zhang, Yanan. 2024. Reducing SULT2B1 promotes the interaction of LncRNAgga3-204 with SMAD4 to inhibit the macrophage inflammatory response and delay atherosclerosis progression. In Translational research : the journal of laboratory and clinical medicine, 268, 13-27. doi:10.1016/j.trsl.2024.01.004. https://pubmed.ncbi.nlm.nih.gov/38286358/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen