C57BL/6JCya-Azin1em1flox/Cya
Common Name:
Azin1-flox
Product ID:
S-CKO-11735
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Azin1-flox
Strain ID
CKOCMP-54375-Azin1-B6J-VA
Gene Name
Product ID
S-CKO-11735
Gene Alias
1700085L02Rik; AZI; Oazi; Oazin
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Azin1em1flox/Cya mice (Catalog S-CKO-11735) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000065308
NCBI RefSeq
NM_018745
Target Region
Exon 5~6
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Azin1, or Antizyme Inhibitor 1, is a cell cycle regulator [1]. It can bind to and inhibit antizyme, a protein that normally functions as an anti-tumor regulator by deactivating growth-promoting proteins such as c-Myc [1].
In medulloblastoma, overexpression of AZIN1 in cells induces phenotypically aggressive features, while knocking-out AZIN1 in tumors via CRISPR-Cas9 in nude mice corresponds with reduced tumor progression and prolonged survival, and its expression directly correlates with MYC amplification status in patients [1]. In colorectal cancer, high levels of AZIN1 RNA editing are associated with poor prognosis, and edited AZIN1 enhances stemness and drives metastasis [2]. In endometrial cancer, ADAR1 expression is correlated with AZIN1 RNA editing level, and both are independent predictors of prognosis, with knockdown of ADAR1 leading to increased apoptosis in cancer cells [3]. In gastric cancer, ADAR1 knockout inhibits cell metastasis and reverses cisplatin resistance by down-regulating AZIN1 expression [4].
In conclusion, Azin1 plays a crucial role in tumor-related biological processes. Gene knockout models, especially in mice, have revealed its significant impact on the pathogenesis and progression of various cancers, including medulloblastoma, colorectal, endometrial, and gastric cancers, highlighting its potential as a biomarker and therapeutic target.
References:
1. Sesen, Julie, Martinez, Tyra, Busatto, Sara, Smith, Edward R, Ghalali, Aram. 2025. AZIN1 level is increased in medulloblastoma and correlates with c-Myc activity and tumor phenotype. In Journal of experimental & clinical cancer research : CR, 44, 56. doi:10.1186/s13046-025-03274-1. https://pubmed.ncbi.nlm.nih.gov/39962590/
2. Shigeyasu, Kunitoshi, Okugawa, Yoshinaga, Toden, Shusuke, Chen, Leilei, Goel, Ajay. 2018. AZIN1 RNA editing confers cancer stemness and enhances oncogenic potential in colorectal cancer. In JCI insight, 3, . doi:10.1172/jci.insight.99976. https://pubmed.ncbi.nlm.nih.gov/29925690/
3. Nakamura, Keiichiro, Shigeyasu, Kunitoshi, Okamoto, Kazuhiro, Matsuoka, Hirofumi, Masuyama, Hisashi. 2022. ADAR1 and AZIN1 RNA editing function as an oncogene and contributes to immortalization in endometrial cancer. In Gynecologic oncology, 166, 326-333. doi:10.1016/j.ygyno.2022.05.026. https://pubmed.ncbi.nlm.nih.gov/35697535/
4. Wang, Honghong, Yang, Lina, Liu, Rui, Zhang, Miao, Xu, Yuanyi. 2023. ADAR1 affects gastric cancer cell metastasis and reverses cisplatin resistance through AZIN1. In Anti-cancer drugs, 34, 1132-1145. doi:10.1097/CAD.0000000000001516. https://pubmed.ncbi.nlm.nih.gov/37104086/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen