C57BL/6JCya-Mkrn1em1flox/Cya
Common Name:
Mkrn1-flox
Product ID:
S-CKO-11771
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mkrn1-flox
Strain ID
CKOCMP-54484-Mkrn1-B6J-VA
Gene Name
Product ID
S-CKO-11771
Gene Alias
RFP
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mkrn1em1flox/Cya mice (Catalog S-CKO-11771) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031985
NCBI RefSeq
NM_018810
Target Region
Exon 3~4
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Mkrn1, also called RNF61, is a member of the RING finger protein family and functions as an E3 ubiquitin ligase. It is involved in multiple biological processes and associated with various diseases. It participates in pathways like TGF-β signalling, which is crucial for cell growth, differentiation, and apoptosis [1].
In colorectal cancer, MKRN1 expression is elevated compared to paracancerous tissues. Conditional knockout mice with intestinal knockout of MKRN1 showed greatly reduced intestinal lesions and metastatic liver microlesions, indicating that high MKRN1 levels promote TGF-β signalling through ubiquitination and degradation of SNIP1, facilitating CRC metastasis [1].
In triple-negative breast cancer, SF3A2 promotes TNBC progression by regulating the alternative splicing of MKRN1 and promoting the expression of the oncogenic isoform MKRN1-T1 [2].
In renal clear cell carcinoma, MKRN1 is lowly expressed, and its overexpression inhibits cell proliferation by arresting the cell cycle, suggesting it acts as a tumor suppressor [3].
In cervical cancer, MKRN1 expression is up-regulated, and its knockdown inhibits cell proliferation, migration, and invasion [4].
In conclusion, Mkrn1 plays diverse roles in different diseases. Its functions range from promoting cancer metastasis in colorectal and cervical cancers to acting as a tumor suppressor in renal clear cell carcinoma. The use of gene knockout and conditional knockout mouse models has been crucial in revealing these functions, providing valuable insights into disease mechanisms and potential therapeutic targets.
References：
1. Zhang, Yi, Li, Qin-Shan, Liu, Hong-Lin, Liu, Li-Hong, Li, Meng-Xing. 2023. MKRN1 promotes colorectal cancer metastasis by activating the TGF-β signalling pathway through SNIP1 protein degradation. In Journal of experimental & clinical cancer research : CR, 42, 219. doi:10.1186/s13046-023-02788-w. https://pubmed.ncbi.nlm.nih.gov/37620897/
2. Deng, Ling, Liao, Li, Zhang, Yin-Ling, Shao, Zhi-Ming, Li, Da-Qiang. 2024. SF3A2 promotes progression and cisplatin resistance in triple-negative breast cancer via alternative splicing of MKRN1. In Science advances, 10, eadj4009. doi:10.1126/sciadv.adj4009. https://pubmed.ncbi.nlm.nih.gov/38569025/
3. Yang, Yun, Luo, Yanyan, Huang, Shuting, Li, Chuanyin, Wang, Chengcheng. . MKRN1/2 serve as tumor suppressors in renal clear cell carcinoma by regulating the expression of p53. In Cancer biomarkers : section A of Disease markers, 36, 267-278. doi:10.3233/CBM-210559. https://pubmed.ncbi.nlm.nih.gov/36938725/
4. Dong, Xiang, Zhan, Yuling, Li, Suwan, Yang, Minghui, Gao, Yu. 2024. MKRN1 regulates the expression profiles and transcription factor activity in HeLa cells inhibition suppresses cervical cancer cell progression. In Scientific reports, 14, 6129. doi:10.1038/s41598-024-56830-8. https://pubmed.ncbi.nlm.nih.gov/38480859/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen