C57BL/6JCya-Calcrlem1flox/Cya
Common Name:
Calcrl-flox
Product ID:
S-CKO-11816
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Calcrl-flox
Strain ID
CKOCMP-54598-Calcrl-B6J-VA
Gene Name
Product ID
S-CKO-11816
Gene Alias
CRLR
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Calcrlem1flox/Cya mice (Catalog S-CKO-11816) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000074262
NCBI RefSeq
NM_018782
Target Region
Exon 6
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
CALCRL, short for calcitonin receptor-like receptor, is a G-protein-coupled neuropeptide receptor. It is involved in various biological processes, and its associated pathways include cyclic AMP (cAMP) signaling pathway, which is activated when it forms a receptor complex with RAMP1 in response to ligands like calcitonin gene-related peptide (CGRP) [1,3,5,6]. It is of great biological importance in processes such as immune response, hematopoiesis, and drug-tolerance in cancer cells. Genetic models, like KO or CKO mouse models, are valuable for studying its functions.
In the spleen, nociceptors release CGRP which acts on B cells through the CALCRL-RAMP1 complex via the cAMP pathway, promoting germinal center response and humoral immunity [1]. In acute myeloid leukemia (AML), CALCRL is expressed in relapse-initiating drug-tolerant cells. Knockdown of CALCRL impairs leukemic growth, decreases the frequency of leukemia-initiating cells, and sensitizes cells to cytarabine [2]. In tendon-bone healing, overexpression of Calcrl in mice improves osteogenic differentiation, type H vessel and nerve fiber intensity at the tendon-bone interface, as well as gait and biomechanical performance [3]. In AML/ETO + AML patients, lower CALCRL expression is associated with longer survival, and its expression decreases after chemotherapy-induced remission [4]. In AML cells, overexpression of CALCRL confers resistance to daunorubicin through up-regulation of the XRCC5/TYK2/JAK1 pathway [7]. In glioma, interfering with CALCRL expression in U87 cells (a glioma cell line) inhibits proliferation, promotes apoptosis, and is related to prognosis, especially in low-grade gliomas [8].
In conclusion, CALCRL plays essential roles in multiple biological processes. Through model-based research, its significance in immune regulation, cancer drug-resistance, and tissue repair has been revealed. In particular, in diseases like AML and glioma, studies using KO/CKO mouse models could potentially provide insights into novel therapeutic strategies targeting CALCRL.
References:
1. Wu, Min, Song, Guangping, Li, Jianing, Zhang, Yucheng, Li, Huiyan. 2024. Innervation of nociceptor neurons in the spleen promotes germinal center responses and humoral immunity. In Cell, 187, 2935-2951.e19. doi:10.1016/j.cell.2024.04.027. https://pubmed.ncbi.nlm.nih.gov/38772371/
2. Larrue, Clément, Guiraud, Nathan, Mouchel, Pierre-Luc, Récher, Christian, Sarry, Jean-Emmanuel. 2021. Adrenomedullin-CALCRL axis controls relapse-initiating drug tolerant acute myeloid leukemia cells. In Nature communications, 12, 422. doi:10.1038/s41467-020-20717-9. https://pubmed.ncbi.nlm.nih.gov/33462236/
3. Zhao, Xibang, Wu, Guanfu, Zhang, Jing, Yu, Ziyi, Wang, Jiali. 2024. Activation of CGRP receptor-mediated signaling promotes tendon-bone healing. In Science advances, 10, eadg7380. doi:10.1126/sciadv.adg7380. https://pubmed.ncbi.nlm.nih.gov/38457499/
4. Wang, Rongrong, Li, Miao, Bai, Yujia, Jiao, Yang, Qi, Xiaofei. 2022. CALCRL Gene is a Suitable Prognostic Factor in AML/ETO+ AML Patients. In Journal of oncology, 2022, 3024360. doi:10.1155/2022/3024360. https://pubmed.ncbi.nlm.nih.gov/35342399/
5. Hou, Yu, Sun, Linyu, LaFleur, Martin W, Sharpe, Arlene H, Kuchroo, Vijay K. 2024. Neuropeptide signalling orchestrates T cell differentiation. In Nature, 635, 444-452. doi:10.1038/s41586-024-08049-w. https://pubmed.ncbi.nlm.nih.gov/39415015/
6. Gao, Xin, Zhang, Dachuan, Xu, Chunliang, Caron, Kathleen M, Frenette, Paul S. 2020. Nociceptive nerves regulate haematopoietic stem cell mobilization. In Nature, 589, 591-596. doi:10.1038/s41586-020-03057-y. https://pubmed.ncbi.nlm.nih.gov/33361809/
7. Tang, Shanhao, Li, Shuangyue, Shi, Xiaowei, Wu, An, Ouyang, Guifang. 2023. CALCRL induces resistance to daunorubicin in acute myeloid leukemia cells through upregulation of XRCC5/TYK2/JAK1 pathway. In Anti-cancer drugs, 35, 163-176. doi:10.1097/CAD.0000000000001547. https://pubmed.ncbi.nlm.nih.gov/37948318/
8. Gu, Shengcai, Shu, Lei, Zhou, Lv, Gao, Peng, Cheng, Hongwei. . Interfering with CALCRL expression inhibits glioma proliferation, promotes apoptosis, and predicts prognosis in low-grade gliomas. In Annals of translational medicine, 10, 1277. doi:10.21037/atm-22-5154. https://pubmed.ncbi.nlm.nih.gov/36618798/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen