C57BL/6JCya-Rcan1em1flox/Cya
Common Name:
Rcan1-flox
Product ID:
S-CKO-11875
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rcan1-flox
Strain ID
CKOCMP-54720-Rcan1-B6J-VA
Gene Name
Product ID
S-CKO-11875
Gene Alias
Adapt78; CALP1L; CSP1; DSC1; Dscr1; MCIP1; RCN1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rcan1em1flox/Cya mice (Catalog S-CKO-11875) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000060005
NCBI RefSeq
NM_001081549.2
Target Region
Exon 2~3
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Rcan1, short for Regulator of calcineurin 1, is a crucial gene with its primary function being the inhibition of the phosphatase calcineurin, and it also regulates mitochondrial function [4]. It is involved in pathways such as the calcineurin/NFAT transcriptional pathway, and plays a significant role in various biological processes including synaptic plasticity, development and maintenance of the cardiovascular system, and is associated with multiple diseases [4,5]. Genetic models like KO/CKO mouse models are valuable for studying Rcan1.
In cardiovascular diseases, RCAN1 reduction protects against atherosclerosis by reducing the uptake of oxidized low-density lipoproteins, while RCAN1 has a protective effect on myocardial ischemia/reperfusion injury, myocardial hypertrophy and intramural hematoma/aortic rupture [1]. In septic cardiomyopathy, RCAN1 -/- mice showed severe cardiac function impairment, increased myocardial hypertrophy and fibrosis, and aggravated mitochondrial injury, indicating that RCAN1 deficiency worsens the condition [3]. In diabetic cardiomyopathy, knockdown of RCAN1 improved cardiac dysfunction, lipid accumulation, and mitochondrial fission in db/db mice [2].
In conclusion, Rcan1 is essential in regulating mitochondrial function and calcineurin activity. Studies using KO/CKO mouse models have revealed its roles in cardiovascular diseases, septic cardiomyopathy, and diabetic cardiomyopathy. Understanding Rcan1 through these models provides insights into the mechanisms of these diseases and potential therapeutic strategies.
References:
1. Wang, Shuai, Wang, Yuqing, Qiu, Kaixin, Zhu, Jin, Wu, Yili. 2020. RCAN1 in cardiovascular diseases: molecular mechanisms and a potential therapeutic target. In Molecular medicine (Cambridge, Mass.), 26, 118. doi:10.1186/s10020-020-00249-0. https://pubmed.ncbi.nlm.nih.gov/33267791/
2. Shu, Songren, Cui, Hao, Liu, Zirui, Hu, Shengshou, Song, Jiangping. 2024. Suppression of RCAN1 alleviated lipid accumulation and mitochondrial fission in diabetic cardiomyopathy. In Metabolism: clinical and experimental, 158, 155977. doi:10.1016/j.metabol.2024.155977. https://pubmed.ncbi.nlm.nih.gov/39053690/
3. Zhuang, Jinqiang, Chen, Liming, Li, Gongke, Wang, Shijun, Yuan, Ruijun. 2022. RCAN1 deficiency aggravates sepsis-induced cardiac remodeling and dysfunction by accelerating mitochondrial pathological fission. In Inflammation research : official journal of the European Histamine Research Society ... [et al.], 71, 1589-1602. doi:10.1007/s00011-022-01628-5. https://pubmed.ncbi.nlm.nih.gov/36305917/
4. Peiris, Heshan, Keating, Damien J. 2017. The neuronal and endocrine roles of RCAN1 in health and disease. In Clinical and experimental pharmacology & physiology, 45, 377-383. doi:10.1111/1440-1681.12884. https://pubmed.ncbi.nlm.nih.gov/29094385/
5. Dudilot, Anthony, Trillaud-Doppia, Emilie, Boehm, Jannic. 2020. RCAN1 Regulates Bidirectional Synaptic Plasticity. In Current biology : CB, 30, 1167-1176.e2. doi:10.1016/j.cub.2020.01.041. https://pubmed.ncbi.nlm.nih.gov/32084406/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen