C57BL/6JCya-Camkk1em1flox/Cya
Common Name:
Camkk1-flox
Product ID:
S-CKO-11905
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Camkk1-flox
Strain ID
CKOCMP-55984-Camkk1-B6J-VA
Gene Name
Product ID
S-CKO-11905
Gene Alias
CaMKKalpha; Camkk; caM-KK 1; caM-KK alpha; caMKK 1; caMKK alpha
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Camkk1em1flox/Cya mice (Catalog S-CKO-11905) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000092937
NCBI RefSeq
NM_018883
Target Region
Exon 3~7
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Camkk1, or calcium/calmodulin-dependent protein kinase kinase 1, phosphorylates and enhances the catalytic activity of downstream kinases such as CaMKI, CaMKIV, and protein kinase B. It is involved in key physiological and pathological processes including tumorigenesis, neuronal morphogenesis, synaptic plasticity, transcription factor activation, and cellular energy homeostasis [4].
In non-alcoholic fatty liver disease (NAFLD), human umbilical cord mesenchymal stem cell-derived exosomes (MSC-ex) can transfer Camkk1, which ameliorates lipid accumulation in an AMP-activated protein kinase-dependent manner, inhibiting SREBP-1C-mediated fatty acid synthesis and enhancing peroxisome proliferator-activated receptor alpha (PPARα)-mediated fatty acid oxidation [1].
In a study on cardiovascular diseases, the single nucleotide polymorphism (SNP) rs7214723 within the Camkk1 gene was investigated, and the results suggested its potential role in CVD susceptibility as a possible genetic biomarker [2].
Regarding lung cancer, the Camkk1 rs7214723 recessive CC genotype was associated with better overall survival, and the Nuclease technology-guided single nucleotide editing demonstrated that the T > C mutation significantly inhibits cell proliferation and migration and promotes cell apoptosis [3].
In conclusion, Camkk1 is crucial in regulating various biological processes. Its role in diseases such as NAFLD, cardiovascular diseases, and lung cancer has been revealed through different research models. These findings help us better understand the molecular mechanisms underlying these diseases and may provide potential therapeutic targets.
References：
1. Yang, Fuji, Wu, Yanshuang, Chen, Yifei, Jin, Jianhua, Yan, Yongmin. 2023. Human umbilical cord mesenchymal stem cell-derived exosomes ameliorate liver steatosis by promoting fatty acid oxidation and reducing fatty acid synthesis. In JHEP reports : innovation in hepatology, 5, 100746. doi:10.1016/j.jhepr.2023.100746. https://pubmed.ncbi.nlm.nih.gov/37274776/
2. Beghi, Sofia, Cavaliere, Francesca, Manfredini, Matteo, Beghi, Cesare, Buschini, Annamaria. . Polymorphism rs7214723 in CAMKK1: a new genetic variant associated with cardiovascular diseases. In Bioscience reports, 41, . doi:10.1042/BSR20210326. https://pubmed.ncbi.nlm.nih.gov/34165505/
3. Zhang, Haorui, Chen, Bocen, Zou, Zixiu, Li, Feng, Wu, Junjie. 2021. Associations Between CAMKK1 Polymorphism rs7214723 and the Prognosis of Patients With Lung Cancer. In Frontiers in oncology, 11, 757484. doi:10.3389/fonc.2021.757484. https://pubmed.ncbi.nlm.nih.gov/34868969/
4. Petrvalska, Olivia, Honzejkova, Karolina, Koupilova, Nicola, Obsilova, Veronika, Obsil, Tomas. . 14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. In Protein science : a publication of the Protein Society, 32, e4805. doi:10.1002/pro.4805. https://pubmed.ncbi.nlm.nih.gov/37817008/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen