C57BL/6JCya-Cxcl13em1flox/Cya
Common Name:
Cxcl13-flox
Product ID:
S-CKO-11906
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cxcl13-flox
Strain ID
CKOCMP-55985-Cxcl13-B6J-VA
Gene Name
Product ID
S-CKO-11906
Gene Alias
4631412M08Rik; ANGIE2; Angie; BCA-1; BLC; BLR1L; Scyb13
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cxcl13em1flox/Cya mice (Catalog S-CKO-11906) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023840
NCBI RefSeq
NM_018866
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Cxcl13, also known as chemokine C-X-C motif ligand 13, is a B-cell chemokine. It binds to its receptor CXCR5, building a signaling network crucial for lymphoid neogenesis, lymphoid organization, and immune responses [1,2].
In a spinal nerve ligation (SNL) mouse model of neuropathic pain, CXCL13 was persistently upregulated in spinal cord neurons, leading to spinal astrocyte activation via CXCR5 and induction of neuropathic pain. shRNA-mediated inhibition of CXCL13 in the spinal cord attenuated SNL-induced neuropathic pain, and spinal overexpression of miR-186-5p, which suppresses CXCL13 expression, alleviated neuropathic pain. Also, neuropathic pain was abrogated in Cxcr5-/-mice [3]. In multiple myeloma, in vivo xenograft models and analysis of patient samples showed that myeloid cells were the main source of increased CXCL13 in MM-infiltrated bone marrow. CXCL13 neutralization blocked RANKL expression and osteoclast formation in vitro, and mice inoculated with CXCL13-silenced MM cells had lower bone marrow disease, reduced M2 macrophage numbers, and decreased bone disease [4].
In conclusion, Cxcl13 is essential for immune-related processes and lymphoid development. Model-based research, especially the use of KO/CKO mouse models, has revealed its role in neuropathic pain and multiple myeloma. These findings suggest that targeting Cxcl13 could be a potential therapeutic approach for these diseases.
References:
1. Pan, Zijian, Zhu, Tong, Liu, Yanjun, Zhang, Nannan. 2022. Role of the CXCL13/CXCR5 Axis in Autoimmune Diseases. In Frontiers in immunology, 13, 850998. doi:10.3389/fimmu.2022.850998. https://pubmed.ncbi.nlm.nih.gov/35309354/
2. Wang, Binhan, Wang, Manni, Ao, Danyi, Wei, Xiawei. 2022. CXCL13-CXCR5 axis: Regulation in inflammatory diseases and cancer. In Biochimica et biophysica acta. Reviews on cancer, 1877, 188799. doi:10.1016/j.bbcan.2022.188799. https://pubmed.ncbi.nlm.nih.gov/36103908/
3. Jiang, Bao-Chun, Cao, De-Li, Zhang, Xin, Ji, Ru-Rong, Gao, Yong-Jing. 2016. CXCL13 drives spinal astrocyte activation and neuropathic pain via CXCR5. In The Journal of clinical investigation, 126, 745-61. doi:10.1172/JCI81950. https://pubmed.ncbi.nlm.nih.gov/26752644/
4. Beider, Katia, Voevoda-Dimenshtein, Valeria, Zoabi, Ali, Peled, Amnon, Nagler, Arnon. 2022. CXCL13 chemokine is a novel player in multiple myeloma osteolytic microenvironment, M2 macrophage polarization, and tumor progression. In Journal of hematology & oncology, 15, 144. doi:10.1186/s13045-022-01366-5. https://pubmed.ncbi.nlm.nih.gov/36217194/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen