C57BL/6JCya-Tmem131em1flox/Cya
Common Name:
Tmem131-flox
Product ID:
S-CKO-11922
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tmem131-flox
Strain ID
CKOCMP-56030-Tmem131-B6J-VA
Gene Name
Product ID
S-CKO-11922
Gene Alias
2610524E03Rik; CC28; D1Bwg0491e; Neg; RW1; YR-23; mKIAA0257
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmem131em1flox/Cya mice (Catalog S-CKO-11922) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027290
NCBI RefSeq
NM_018872
Target Region
Exon 5
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Tmem131 is a gene with important roles in intracellular processes. Its amino termini contain bacterial PapD chaperone-like domains, while its carboxy termini interact with TRAPPC8. It is involved in intracellular collagen assembly and secretory cargo trafficking, playing a part in the recruitment of premature collagen monomers for proper assembly and secretion, and driving collagen cargo trafficking from the endoplasmic reticulum (ER) to the Golgi [1].
Knockdown of Tmem131 in Caenorhabditis elegans impairs collagen production and activates ER stress response, indicating its essential role in collagen-related processes [1]. In both C. elegans and human sarcoma cells, knockdown of Tmem131 results in reduced formation of large COPII vesicles associated with extracellular matrix (ECM) protein secretion, suggesting a cooperative role with TMEM39A in the secretion of extracellular components [2].
In conclusion, Tmem131 is crucial for intracellular collagen assembly and secretory cargo trafficking. Studies in C. elegans through knockdown (a form of loss-of-function experiment) have revealed its significance in collagen-related biological processes. These findings may have implications for understanding diseases related to collagen dysregulation, such as pathological tissue fibrosis [1].
References:
1. Zhang, Zhe, Bai, Meirong, Barbosa, Guilherme Oliveira, Kornberg, Thomas B, Ma, Dengke K. 2020. Broadly conserved roles of TMEM131 family proteins in intracellular collagen assembly and secretory cargo trafficking. In Science advances, 6, eaay7667. doi:10.1126/sciadv.aay7667. https://pubmed.ncbi.nlm.nih.gov/32095531/
2. Sung, Jee Young, Lim, Ga-Eun, Goo, Jarim, Kim, Yong-Nyun, Shim, Jaegal. 2024. TMEM39A and TMEM131 facilitate bulk transport of ECM proteins through large COPII vesicle formation. In Journal of genetics and genomics = Yi chuan xue bao, 52, 189-203. doi:10.1016/j.jgg.2024.10.013. https://pubmed.ncbi.nlm.nih.gov/39521045/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen