C57BL/6JCya-Ralaem1flox/Cya
Common Name:
Rala-flox
Product ID:
S-CKO-11929
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Rala-flox
Strain ID
CKOCMP-56044-Rala-B6J-VA
Gene Name
Product ID
S-CKO-11929
Gene Alias
3010001O15Rik; Ral; Rasl1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ralaem1flox/Cya mice (Catalog S-CKO-11929) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000009003
NCBI RefSeq
NM_019491
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Rala, a member of the small GTPase Ras superfamily, acts as a molecular switch toggling between inactive GDP-bound and active GTP-bound states. It is involved in diverse cellular functions such as vesicle trafficking, filopodia formation, mitochondrial fission, and cytokinesis [3]. It participates in multiple pathways like those related to exocytosis through interacting with the exocyst complex [5]. Rala is of great biological importance as it is associated with various physiological and pathological processes. Genetic models, especially KO/CKO mouse models, are valuable for studying its functions.
In white adipocytes, deletion of Rala in mice prevents mitochondrial fragmentation caused by high-fat diet (HFD), reduces HFD-induced weight gain by increasing fatty acid oxidation. Mechanistically, Rala increases mitochondrial fission by reversing the inhibitory Ser637 phosphorylation of the fission protein Drp1 [1]. In breast cancer, inhibition of RalA-GTP form attenuates the interaction between ANXA2 and Src, which is part of a complex promoting CTC formation and metastasis [2]. In osteosarcoma, knocking down Rala could potentially impact tumor cell proliferation, migration, and invasion as it is highly expressed in human osteosarcoma tissues and cell lines [4].
In conclusion, Rala plays essential roles in multiple biological processes. Studies using KO/CKO mouse models have revealed its significance in obesity-related metabolic dysfunction, breast cancer metastasis, and osteosarcoma development. Understanding Rala's functions can provide new insights into these disease mechanisms and potentially lead to novel therapeutic strategies.
References:
1. Xia, Wenmin, Veeragandham, Preethi, Cao, Yu, Huang, Jianfeng, Saltiel, Alan R. 2024. Obesity causes mitochondrial fragmentation and dysfunction in white adipocytes due to RalA activation. In Nature metabolism, 6, 273-289. doi:10.1038/s42255-024-00978-0. https://pubmed.ncbi.nlm.nih.gov/38286821/
2. Long, Yufei, Chong, Tuotuo, Lyu, Xiaoming, Chen, Ceshi, Li, Xin. 2022. FOXD1-dependent RalA-ANXA2-Src complex promotes CTC formation in breast cancer. In Journal of experimental & clinical cancer research : CR, 41, 301. doi:10.1186/s13046-022-02504-0. https://pubmed.ncbi.nlm.nih.gov/36229838/
3. Richardson, Dillon S, Spehar, Jonathan M, Han, David T, Chakravarthy, Prathik A, Sizemore, Steven T. 2022. The RAL Enigma: Distinct Roles of RALA and RALB in Cancer. In Cells, 11, . doi:10.3390/cells11101645. https://pubmed.ncbi.nlm.nih.gov/35626682/
4. Fan, Gentao, Zhu, Yan, Zhu, Hao, Zhao, Jianning, Wang, Yicun. 2023. Identification of RALA as a Therapeutic Target and Prognostic Predictor of Osteosarcoma. In BioMed research international, 2023, 1150768. doi:10.1155/2023/1150768. https://pubmed.ncbi.nlm.nih.gov/36817861/
5. Wang, Shifeng, Chen, Xu, Crisman, Lauren, Kennedy, Matthew J, Shen, Jingshi. 2023. Regulation of cargo exocytosis by a Reps1-Ralbp1-RalA module. In Science advances, 9, eade2540. doi:10.1126/sciadv.ade2540. https://pubmed.ncbi.nlm.nih.gov/36812304/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen