C57BL/6JCya-Ramp3em1flox/Cya
Common Name
Ramp3-flox
Product ID
S-CKO-11948
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-56089-Ramp3-B6J-VA
Status
When using this mouse strain in a publication, please cite “Ramp3-flox Mouse (Catalog S-CKO-11948) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Ramp3-flox
Strain ID
CKOCMP-56089-Ramp3-B6J-VA
Gene Name
Product ID
S-CKO-11948
Gene Alias
--
Background
C57BL/6JCya
Gene Full Name
receptor (calcitonin) activity modifying protein 3
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 11 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000045374
NCBI Transcript ID
NM_019511
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Overview of Gene Research
RAMP3, short for Receptor Activity-Modifying Protein 3, is an accessory protein that interacts with G-protein coupled receptors (GPCRs) [4]. It plays diverse roles in multiple biological processes. RAMP3 is involved in neuropeptide signalling, regulating T cell differentiation. It is also a component of the receptor for neuropeptide CGRP, where extracellular CGRP signalling through the RAMP3-CALCRL receptor restricts TH2 cell differentiation and promotes TH1 cell differentiation [1]. In addition, RAMP3 is related to the adrenomedullin system, which is crucial for maintaining cardiovascular homeostasis, energy metabolism, and anti-tumour functions [2,3,5].
In gene knockout mouse models, RAMP3-/-mice have shown significant phenotypes. In the context of cardiovascular stress, RAMP3-/-mice show reduced systolic function and enhanced fibrosis after transverse aortic constriction (TAC), with a reduction in cardiac lymphatic vessels being a characteristic feature, indicating its role in later adaptation to cardiovascular stress [2]. RAMP3-/-OVX mice exhibit greater obesity, higher serum insulin levels, and exacerbated hepatic steatosis, suggesting its role in energy metabolism and hepatoprotection in postmenopausal obesity [3]. In tumour-related studies, RAMP3-/-mice have suppressed tumour metastasis as the number of podoplanin-positive cancer-associated fibroblasts is reduced at metastatic sites [5].
In conclusion, RAMP3 is essential in regulating T cell differentiation, cardiovascular homeostasis, energy metabolism, and tumour-related processes. The study of RAMP3 knockout mouse models has significantly contributed to understanding its functions in these disease-associated biological processes, providing potential therapeutic targets for treating conditions such as postmenopausal obesity, cardiovascular diseases, and cancer metastasis.
References：
1. Hou, Yu, Sun, Linyu, LaFleur, Martin W, Sharpe, Arlene H, Kuchroo, Vijay K. 2024. Neuropeptide signalling orchestrates T cell differentiation. In Nature, 635, 444-452. doi:10.1038/s41586-024-08049-w. https://pubmed.ncbi.nlm.nih.gov/39415015/
2. Cui, Nanqi, Sakurai, Takayuki, Kamiyoshi, Akiko, Yamada, Mitsuhiko, Shindo, Takayuki. . Adrenomedullin-RAMP2 and -RAMP3 Systems Regulate Cardiac Homeostasis during Cardiovascular Stress. In Endocrinology, 162, . doi:10.1210/endocr/bqab001. https://pubmed.ncbi.nlm.nih.gov/33545715/
3. Liu, Teng, Kamiyoshi, Akiko, Tanaka, Megumu, Yamauchi, Akihiro, Shindo, Takayuki. 2018. RAMP3 deficiency enhances postmenopausal obesity and metabolic disorders. In Peptides, 110, 10-18. doi:10.1016/j.peptides.2018.10.006. https://pubmed.ncbi.nlm.nih.gov/30385288/
4. Prakash, Jai, Herlin, Maria, Kumar, Jitender, Richards, Gareth O, McGuigan, Fiona E A. 2019. Analysis of RAMP3 gene polymorphism with body composition and bone density in young and elderly women. In Gene, 721S, 100009. doi:10.1016/j.gene.2019.100009. https://pubmed.ncbi.nlm.nih.gov/34530989/
5. Dai, Kun, Tanaka, Megumu, Kamiyoshi, Akiko, Matsui, Shuhei, Shindo, Takayuki. 2019. Deficiency of the adrenomedullin-RAMP3 system suppresses metastasis through the modification of cancer-associated fibroblasts. In Oncogene, 39, 1914-1930. doi:10.1038/s41388-019-1112-z. https://pubmed.ncbi.nlm.nih.gov/31754214/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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