C57BL/6JCya-Becn1em1flox/Cya
Common Name:
Becn1-flox
Product ID:
S-CKO-11978
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Becn1-flox
Strain ID
CKOCMP-56208-Becn1-B6J-VA
Gene Name
Product ID
S-CKO-11978
Gene Alias
Atg6
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Becn1em1flox/Cya mice (Catalog S-CKO-11978) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000130916
NCBI RefSeq
NM_019584
Target Region
Exon 4~7
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Becn1, encoding the Beclin-1 protein, is a well-established regulator of the autophagic pathway and a mammalian orthologue of the yeast ATG6 gene [7]. As a core component of the class III phosphatidylinositol 3-kinase complexes, it mediates autophagy, which is crucial for maintaining cellular homeostasis. Besides autophagy, Becn1 is also involved in processes like apoptosis, embryogenesis, and innate immune responses, and is implicated in diseases such as cancer, neurological disorders, and viral infections [7,8].
CUL3, an E3 ubiquitin ligase, can interact with BECN1, promoting its K48-linked ubiquitination and degradation, thus inhibiting autophagy and promoting tumor progression in breast and ovarian cancer [1]. IL-6 activates autophagy through the IL-6/JAK2/BECN1 pathway in colorectal cancer, with JAK2 phosphorylating BECN1 at Y333, which is crucial for BECN1 activation and IL-6-induced autophagy [2]. AMPK-mediated phosphorylation of BECN1 at Ser90/93/96 promotes ferroptosis by directly blocking system Xc-activity [3]. VCP/p97 UFMylation stabilizes BECN1 and facilitates autophagy initiation [4]. TRIM59 regulates autophagy by modulating both the transcription and the ubiquitination of BECN1 in non-small cell lung cancer [5]. USP19 affects the ubiquitination of BECN1, promoting autophagosome formation and inhibiting DDX58/RIG-I-mediated type I interferon signaling [6].
In conclusion, Becn1 is a key regulator of autophagy and is involved in multiple biological processes and disease conditions. Studies on Becn1, including those using gene-knockout or conditional-knockout mouse models, have enhanced our understanding of its role in diseases such as cancer, neurodegenerative disorders, and viral infections, providing potential therapeutic strategies.
References:
1. Li, Xuan, Yang, Kai-Bin, Chen, Wei, Li, Jun-Dong, Zhu, Xiao-Feng. 2021. CUL3 (cullin 3)-mediated ubiquitination and degradation of BECN1 (beclin 1) inhibit autophagy and promote tumor progression. In Autophagy, 17, 4323-4340. doi:10.1080/15548627.2021.1912270. https://pubmed.ncbi.nlm.nih.gov/33977871/
2. Hu, Fuqing, Song, Da, Yan, Yumeng, Hu, Junbo, Wang, Guihua. 2021. IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation. In Nature communications, 12, 3651. doi:10.1038/s41467-021-23923-1. https://pubmed.ncbi.nlm.nih.gov/34131122/
3. Song, Xinxin, Zhu, Shan, Chen, Pan, Kang, Rui, Tang, Daolin. 2018. AMPK-Mediated BECN1 Phosphorylation Promotes Ferroptosis by Directly Blocking System Xc- Activity. In Current biology : CB, 28, 2388-2399.e5. doi:10.1016/j.cub.2018.05.094. https://pubmed.ncbi.nlm.nih.gov/30057310/
4. Wang, Zhifeng, Xiong, Shuhui, Wu, Zhaoyi, Zhu, Wei-Guo, Xu, Xingzhi. 2024. VCP/p97 UFMylation stabilizes BECN1 and facilitates the initiation of autophagy. In Autophagy, 20, 2041-2054. doi:10.1080/15548627.2024.2356488. https://pubmed.ncbi.nlm.nih.gov/38762759/
5. Han, Tianyu, Guo, Meng, Gan, Mingxi, Tian, Xiaoli, Wang, Jian-Bin. 2018. TRIM59 regulates autophagy through modulating both the transcription and the ubiquitination of BECN1. In Autophagy, 14, 2035-2048. doi:10.1080/15548627.2018.1491493. https://pubmed.ncbi.nlm.nih.gov/30231667/
6. Cui, Jun, Jin, Shouheng, Wang, Rong-Fu. 2016. The BECN1-USP19 axis plays a role in the crosstalk between autophagy and antiviral immune responses. In Autophagy, 12, 1210-1. doi:10.1080/15548627.2016.1173801. https://pubmed.ncbi.nlm.nih.gov/27096686/
7. Sahni, Sumit, Merlot, Angelica M, Krishan, Sukriti, Jansson, Patric J, Richardson, Des R. 2014. Gene of the month: BECN1. In Journal of clinical pathology, 67, 656-60. doi:10.1136/jclinpath-2014-202356. https://pubmed.ncbi.nlm.nih.gov/24811486/
8. Mei, Yang, Glover, Karen, Su, Minfei, Sinha, Sangita C. 2016. Conformational flexibility of BECN1: Essential to its key role in autophagy and beyond. In Protein science : a publication of the Protein Society, 25, 1767-85. doi:10.1002/pro.2984. https://pubmed.ncbi.nlm.nih.gov/27414988/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen