C57BL/6JCya-Ak3em1flox/Cya
Common Name:
Ak3-flox
Product ID:
S-CKO-11996
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ak3-flox
Strain ID
CKOCMP-56248-Ak3-B6J-VA
Gene Name
Product ID
S-CKO-11996
Gene Alias
AK-3; Ak3l; Ak3l1; Akl3l
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ak3em1flox/Cya mice (Catalog S-CKO-11996) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025696
NCBI RefSeq
NM_021299
Target Region
Exon 3
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Ak3, also known as adenylate kinase 3, is an enzyme with multiple functions. In the citric acid cycle, it is responsible for GTP/GDP recycling, using GTP to phosphorylate AMP [2]. In the ciliate Paramecium tetraurelia, AK3, one of its four arginine kinase genes, is likely located in the ciliary membrane and influences swimming velocity through the phosphoarginine shuttle system [3].
In hepatocellular carcinoma, METTL3, an m6A methyltransferase, directly modulates the stability of AK3 via m6A modification, affecting the proliferation and metastasis of the cancer [1]. In breast cancer, miR-96-5p negatively correlates with AK3 gene expression, and AK3 is positively associated with the overall survival of breast cancer patients. miR-96-5p promotes breast cancer cell migration through targeting AK3 and activating the MEK/ERK signaling pathway [4]. In Parkinson's disease, AK3 was identified as a biomarker through bioinformatics analysis, which may be associated with the disease and related functional pathways [5].
In conclusion, Ak3 is involved in crucial biological processes such as the citric acid cycle and ciliary function. In diseases, it plays significant roles in hepatocellular carcinoma, breast cancer, and Parkinson's disease. Studies on Ak3 contribute to understanding disease mechanisms, potentially providing new directions for treatment strategies.
References:
1. Yang, Weipeng, Liu, Xilin, Huang, Yunmei, Wu, Xianjian, Luo, Chunying. 2024. METTL3 alters AK3 RNA expression in an m6A-dependent manner to affect the proliferation and metastasis of hepatocellular carcinoma. In International journal of biological macromolecules, 282, 137213. doi:10.1016/j.ijbiomac.2024.137213. https://pubmed.ncbi.nlm.nih.gov/39515695/
2. Rogne, Per, Dulko-Smith, Beata, Goodman, Jack, Sauer-Eriksson, A Elisabeth, Wolf-Watz, Magnus. 2020. Structural Basis for GTP versus ATP Selectivity in the NMP Kinase AK3. In Biochemistry, 59, 3570-3581. doi:10.1021/acs.biochem.0c00549. https://pubmed.ncbi.nlm.nih.gov/32822537/
3. Yano, Daichi, Funadani, Ryouji, Uda, Kouji, Matsuoka, Tatsuomi, Suzuki, Tomohiko. 2020. Role of arginine kinase in Paramecium tetraurelia (Ciliophora, Peniculida): Subcellular localization of AK3 and phosphoarginine shuttle system in cilia. In European journal of protistology, 74, 125705. doi:10.1016/j.ejop.2020.125705. https://pubmed.ncbi.nlm.nih.gov/32464434/
4. Qin, Wei-Yan, Feng, Shi-Chun, Sun, Yong-Qiang, Jiang, Guo-Qin. 2020. MiR-96-5p promotes breast cancer migration by activating MEK/ERK signaling. In The journal of gene medicine, 22, e3188. doi:10.1002/jgm.3188. https://pubmed.ncbi.nlm.nih.gov/32196830/
5. Zhang, Jiyuan, Ma, Xiaopeng, Li, Zhiguang, Wang, Shan, Wang, Liang. 2024. Identification of key genes and diagnostic model associated with circadian rhythms and Parkinson's disease by bioinformatics analysis. In Frontiers in aging neuroscience, 16, 1458476. doi:10.3389/fnagi.2024.1458476. https://pubmed.ncbi.nlm.nih.gov/39478700/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen