C57BL/6JCya-Dmrtb1em1flox/Cya
Common Name:
Dmrtb1-flox
Product ID:
S-CKO-12013
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Dmrtb1-flox
Strain ID
CKOCMP-56296-Dmrtb1-B6J-VA
Gene Name
Product ID
S-CKO-12013
Gene Alias
Dmrt6; Prp13
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dmrtb1em1flox/Cya mice (Catalog S-CKO-12013) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069271
NCBI RefSeq
NM_019872
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Dmrtb1, also known as DMRT6, belongs to the DMRT family. It plays critical functions in sex determination/differentiation, gonadal development, and functional maintenance, and is involved in the transition between mitosis and meiosis in germ cells [1,2,6]. It may also have a role in the evolution of tetrapod testes size [3].
In the large yellow croaker (Larimichthys crocea), Lcdmrtb1 (a dmrtb1 homolog) was highly expressed in the testis. RNA interference of Lcdmrtb1 in testis cells led to decreased expression of related genes like Lcdmrt1, sox9a/b, and amh, altered cell morphology, reduced growth rate, disappearance of spermatogonia, and reduction of Sertoli cells [1]. In mice, a null mutation in Dmrt6 disrupted spermatogonial differentiation in C57BL/6J strain, causing inappropriate expression of differentiation and meiotic initiation factors and apoptosis of late spermatogonia. In 129Sv background mice, Dmrt6 mutant germ cells had defects in meiotic chromosome pairing and mainly arrested in mid-pachynema [6]. Deletion of an evolutionarily conserved topologically associated domain boundary near Dmrtb1 in mice led to significant changes in testis gene expression, abnormal histology, reduced efficiency of meiosis and spermatogenesis, and decreased daily sperm production [4,5].
In conclusion, Dmrtb1 is crucial for testicular development and the transition between mitosis and meiosis in germ cells. Studies using gene-knockout mouse models have revealed its role in spermatogenesis, and its disruption can lead to abnormal testis function, highlighting its importance in male fertility-related research [1,4,5,6].
References:
1. Feng, Yan, Zhong, Zhaowei, Wan, Haifu, Jiang, Yonghua, Wang, Yilei. 2023. dmrtb1 is involved in the testicular development in Larimichthys crocea. In Reproduction (Cambridge, England), 165, 159-170. doi:10.1530/REP-22-0214. https://pubmed.ncbi.nlm.nih.gov/36342669/
2. Hilbold, E, Bergmann, M, Fietz, D, Rode, K, Brehm, R. 2019. Immunolocalization of DMRTB1 in human testis with normal and impaired spermatogenesis. In Andrology, 7, 428-440. doi:10.1111/andr.12617. https://pubmed.ncbi.nlm.nih.gov/30920770/
3. Baker, Joanna, Meade, Andrew, Venditti, Chris. 2021. Genes underlying the evolution of tetrapod testes size. In BMC biology, 19, 162. doi:10.1186/s12915-021-01107-z. https://pubmed.ncbi.nlm.nih.gov/34407824/
4. Lima, Ana C, Okhovat, Mariam, Stendahl, Alexandra M, Conrad, Donald F, Carbone, Lucia. . Deletion of an evolutionarily conserved TAD boundary impacts spermatogenesis in mice†. In Biology of reproduction, 112, 767-779. doi:10.1093/biolre/ioaf017. https://pubmed.ncbi.nlm.nih.gov/39903672/
5. Lima, Ana C, Okhovat, Mariam, Stendahl, Alexandra M, Conrad, Donald F, Carbone, Lucia. 2024. Deletion of an evolutionarily conserved TAD boundary compromises spermatogenesis in mice. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.07.08.602428. https://pubmed.ncbi.nlm.nih.gov/39026739/
6. Zhang, Teng, Murphy, Mark W, Gearhart, Micah D, Bardwell, Vivian J, Zarkower, David. . The mammalian Doublesex homolog DMRT6 coordinates the transition between mitotic and meiotic developmental programs during spermatogenesis. In Development (Cambridge, England), 141, 3662-71. doi:10.1242/dev.113936. https://pubmed.ncbi.nlm.nih.gov/25249458/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen