C57BL/6NCya-Pus1em1flox/Cya
Common Name:
Pus1-flox
Product ID:
S-CKO-12048
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pus1-flox
Strain ID
CKOCMP-56361-Pus1-B6N-VA
Gene Name
Product ID
S-CKO-12048
Gene Alias
A730013B20Rik; MPUS1; mPus1p
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Pus1em1flox/Cya mice (Catalog S-CKO-12048) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000086643
NCBI RefSeq
NM_001025561
Target Region
Exon 5~7
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
PUS1, encoding the nuclear PUS1 enzyme, is located in both the nucleus and the mitochondria. It belongs to the family of PUS enzymes that participate in RNA modifications, converting uridine into pseudouridine in several cytosolic and mitochondrial transfer RNA positions, thus increasing the efficiency of protein synthesis in both compartments. PUS1 is involved in various biological processes and has been linked to important pathways like those related to cell proliferation, migration, and translation of oncogenic mRNAs [2,3,4,6].
In prostate cancer, knockdown of PUS1 inhibited bone metastasis independently of its enzymatic activity, with EIF3b as a downstream effector protected from ubiquitin-mediated degradation by PUS1 [1]. In hepatocellular carcinoma, PUS1 overexpression promoted cell proliferation and tumor growth, and knockdown had the opposite effects. PUS1 incorporated pseudouridine into mRNAs of oncogenes, enhancing their translation [6]. In breast cancer, high PUS1 levels were associated with poor outcomes and triple-negative status, and knockdown suppressed cell proliferation, colony formation, and invasion [5]. In non-small cell lung cancer (NSCLC), high PUS1 expression was associated with shorter survival, and it may be involved in NSCLC malignancy and immune cell infiltration [7]. In MLASA syndrome, mutations in PUS1 led to impaired erythropoiesis, anemia, and compromised mitochondrial function [8].
In conclusion, PUS1 plays crucial roles in RNA modification and protein synthesis, affecting various biological processes. Through gene knockout or knockdown studies in different disease models, it has been shown to be involved in cancer progression, such as in prostate, liver, breast, and lung cancers, as well as in the pathogenesis of MLASA syndrome. These findings highlight its potential as a biomarker and therapeutic target in related diseases.
References:
1. Wu, Yongxin, Peng, Shengmeng, Cheng, Bisheng, Lai, Yiming, Huang, Hai. 2024. FOXA1-dependent PUS1 regulates EIF3b stability in a non-enzymatic pathway mediating prostate cancer bone metastasis. In International journal of biological sciences, 20, 4566-4584. doi:10.7150/ijbs.100905. https://pubmed.ncbi.nlm.nih.gov/39247811/
2. Oncul, Ummuhan, Unal-Ince, Elif, Kuloglu, Zarife, Kaygusuz, Gulsah, Eminoglu, Fatma T. . A Novel PUS1 Mutation in 2 Siblings with MLASA Syndrome: A Review of the Literature. In Journal of pediatric hematology/oncology, 43, e592-e595. doi:10.1097/MPH.0000000000001806. https://pubmed.ncbi.nlm.nih.gov/32287105/
3. Li, Lin, Zhu, Chongying, Xu, Shouying, Cui, Xingang, Tang, Chao. 2023. PUS1 is a novel biomarker for evaluating malignancy of human renal cell carcinoma. In Aging, 15, 5215-5227. doi:10.18632/aging.204799. https://pubmed.ncbi.nlm.nih.gov/37315299/
4. Lan, Chenlu, Huang, Xinlei, Liao, Xiwen, Wang, Jianyao, Zhu, Guangzhi. 2023. PUS1 May Be a Potential Prognostic Biomarker and Therapeutic Target for Hepatocellular Carcinoma. In Pharmacogenomics and personalized medicine, 16, 337-355. doi:10.2147/PGPM.S405621. https://pubmed.ncbi.nlm.nih.gov/37091827/
5. Fang, Zheng, Shen, Hong-Yu, Xu, Qi, Zhu, Zhen, Tang, Jin-Hai. 2022. PUS1 is a novel biomarker for predicting poor outcomes and triple-negative status in breast cancer. In Frontiers in oncology, 12, 1030571. doi:10.3389/fonc.2022.1030571. https://pubmed.ncbi.nlm.nih.gov/36457503/
6. Hu, Yan-Xia, Diao, Li-Ting, Hou, Ya-Rui, Ren, Ya-Han, Xiao, Zhen-Dong. 2023. Pseudouridine synthase 1 promotes hepatocellular carcinoma through mRNA pseudouridylation to enhance the translation of oncogenic mRNAs. In Hepatology (Baltimore, Md.), 80, 1058-1073. doi:10.1097/HEP.0000000000000702. https://pubmed.ncbi.nlm.nih.gov/38015993/
7. Tan, Yonghuang, Wang, Zhaotong, Wang, Yingzhao, Wu, Guoyong, Lu, Jianjun. 2023. Multi-omics analysis reveals PUS1 triggered malignancy and correlated with immune infiltrates in NSCLC. In Aging, 15, 12136-12154. doi:10.18632/aging.205169. https://pubmed.ncbi.nlm.nih.gov/37925171/
8. Wang, Bichen, Shi, Deyang, Yang, Shuang, Chu, Yajing, Shi, Jun. . Mitochondrial tRNA pseudouridylation governs erythropoiesis. In Blood, 144, 657-671. doi:10.1182/blood.2023022004. https://pubmed.ncbi.nlm.nih.gov/38635773/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen