C57BL/6JCya-Adarem1flox/Cya
Common Name:
Adar-flox
Product ID:
S-CKO-12082
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Adar-flox
Strain ID
CKOCMP-56417-Adar-B6J-VA
Gene Name
Product ID
S-CKO-12082
Gene Alias
Adar1; Adar1p110; Adar1p150; DRADA; mZaADAR
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adarem1flox/Cya mice (Catalog S-CKO-12082) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000098924
NCBI RefSeq
NM_001038587
Target Region
Exon 4~6
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Adar, short for adenosine deaminase RNA specific, is a gene encoding enzymes that convert adenosine to inosine in double-stranded RNA (dsRNA). This A-I RNA editing is crucial for protein diversity, neuronal network sophistication, and is involved in pathways like immunity, RNA interference [1,3,4,5,6,7]. Genetic models, such as Drosophila, have been valuable in studying Adar's functions [4].
In mouse models, mutations in Adar1 lead to aberrant interferon expression in Aicardi Goutières syndrome, a congenital encephalopathy. Rescues of Adar1 mutant phenotypes by preventing signaling from antiviral RIG-I-like Sensors (RLRs) suggest Adar1's role in innate immune suppression [6]. In Drosophila, Adar mutants show locomotion defects and increased sleep pressure due to failure in homeostatic synaptic processes [6].
In conclusion, Adar is essential for RNA editing, which impacts various biological processes. Mouse and Drosophila models have revealed its significance in innate immunity, neural functions, and sleep regulation. Understanding Adar could potentially lead to new therapeutic strategies for related diseases [1,2,5,6,7,8].
References:
1. Ashley, Carolyn N, Broni, Emmanuel, Miller, Whelton A. 2024. ADAR Family Proteins: A Structural Review. In Current issues in molecular biology, 46, 3919-3945. doi:10.3390/cimb46050243. https://pubmed.ncbi.nlm.nih.gov/38785511/
2. Herbert, Alan. 2019. ADAR and Immune Silencing in Cancer. In Trends in cancer, 5, 272-282. doi:10.1016/j.trecan.2019.03.004. https://pubmed.ncbi.nlm.nih.gov/31174840/
3. Nishikura, Kazuko. . Functions and regulation of RNA editing by ADAR deaminases. In Annual review of biochemistry, 79, 321-49. doi:10.1146/annurev-biochem-060208-105251. https://pubmed.ncbi.nlm.nih.gov/20192758/
4. Keegan, Liam, Khan, Anzer, Vukic, Dragana, O'Connell, Mary. 2017. ADAR RNA editing below the backbone. In RNA (New York, N.Y.), 23, 1317-1328. doi:10.1261/rna.060921.117. https://pubmed.ncbi.nlm.nih.gov/28559490/
5. Piontkivska, Helen, Wales-McGrath, Benjamin, Miyamoto, Michael, Wayne, Marta L. . ADAR Editing in Viruses: An Evolutionary Force to Reckon with. In Genome biology and evolution, 13, . doi:10.1093/gbe/evab240. https://pubmed.ncbi.nlm.nih.gov/34694399/
6. Sinigaglia, Ketty, Wiatrek, Dagmara, Khan, Anzer, O'Connell, Mary A, Keegan, Liam P. 2018. ADAR RNA editing in innate immune response phasing, in circadian clocks and in sleep. In Biochimica et biophysica acta. Gene regulatory mechanisms, 1862, 356-369. doi:10.1016/j.bbagrm.2018.10.011. https://pubmed.ncbi.nlm.nih.gov/30391332/
7. Weng, Shenghui, Yang, Xinyi, Yu, Nannan, Xiong, Sidong, Ruan, Hang. 2023. Harnessing ADAR-Mediated Site-Specific RNA Editing in Immune-Related Disease: Prediction and Therapeutic Implications. In International journal of molecular sciences, 25, . doi:10.3390/ijms25010351. https://pubmed.ncbi.nlm.nih.gov/38203521/
8. Zhang, Yue, Qian, Huizhu, Xu, Jing, Gao, Wen. . ADAR, the carcinogenesis mechanisms of ADAR and related clinical applications. In Annals of translational medicine, 7, 686. doi:10.21037/atm.2019.11.06. https://pubmed.ncbi.nlm.nih.gov/31930087/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen