C57BL/6JCya-Diaph3em1flox/Cya
Common Name:
Diaph3-flox
Product ID:
S-CKO-12084
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Diaph3-flox
Strain ID
CKOCMP-56419-Diaph3-B6J-VA
Gene Name
Product ID
S-CKO-12084
Gene Alias
4930417P13Rik; Dia2; Diap3; Drf3; p134MDia2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Diaph3em1flox/Cya mice (Catalog S-CKO-12084) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000168889
NCBI RefSeq
NM_019670
Target Region
Exon 6~7
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
DIAPH3, a member of the formin proteins, has the capacity to nucleate and elongate actin filaments, thus remodeling the cytoskeleton [2]. It is involved in pathways such as the ERK signaling pathway [1,3]. DIAPH3 is essential for cytokinesis, and its dysfunction can lead to multinucleated cells. It also plays a role in mitosis by regulating the assembly and bipolarity of the mitotic spindle [2].
In hepatocellular carcinoma (HCC), LINC01089, a super enhancer-driven lncRNA, can induce alternative splicing of DIAPH3 by interacting with hnRNPM, leading to skipping of exon 3. Loss of LINC01089 increases DIAPH3 protein levels, suppressing the ERK/Elk1/Snail axis and inhibiting EMT of HCC cells [1]. In lung adenocarcinoma, DIAPH3 is up-regulated and promotes cell growth by interacting with STK38, impairing the interaction between STK38 and MEKK, and activating ERK signaling [3]. In pancreatic cancer, DIAPH3 is highly expressed and promotes cancer progression by activating selenoprotein TrxR1-mediated antioxidant effects [4]. In colorectal cancer, DIAPH3 is down-regulated, and its knockdown promotes cell proliferation and migration by inhibiting EGFR transportation to lysosome [5]. Conditional inactivation of Diaph3 in mouse cerebral cortex disrupts neurogenesis, leading to cortical malformation and autistic-like behavior [2].
In conclusion, DIAPH3 is crucial for cytoskeleton remodeling, cytokinesis, and mitosis. Its dysregulation is associated with various cancers including HCC, lung, pancreatic, and colorectal cancers, as well as neurodevelopmental disorders. Studies using conditional knockout mouse models have revealed its role in these disease conditions, providing insights into the underlying molecular mechanisms and potential therapeutic targets.
References:
1. Su, Tao, Zhang, Nasha, Wang, Teng, Han, Linyu, Yang, Ming. . Super Enhancer-Regulated LncRNA LINC01089 Induces Alternative Splicing of DIAPH3 to Drive Hepatocellular Carcinoma Metastasis. In Cancer research, 83, 4080-4094. doi:10.1158/0008-5472.CAN-23-0544. https://pubmed.ncbi.nlm.nih.gov/37756562/
2. Lau, Eva On-Chai, Damiani, Devid, Chehade, Georges, Gailly, Philippe, Tissir, Fadel. 2021. DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction. In eLife, 10, . doi:10.7554/eLife.61974. https://pubmed.ncbi.nlm.nih.gov/33899739/
3. Xiang, Guo, Weiwei, He, Erji, Gao, Haitao, Ma. 2019. DIAPH3 promotes the tumorigenesis of lung adenocarcinoma. In Experimental cell research, 385, 111662. doi:10.1016/j.yexcr.2019.111662. https://pubmed.ncbi.nlm.nih.gov/31586548/
4. Rong, Yefei, Gao, Jie, Kuang, Tiantao, Li, Zhi, Lou, Wenhui. 2020. DIAPH3 promotes pancreatic cancer progression by activating selenoprotein TrxR1-mediated antioxidant effects. In Journal of cellular and molecular medicine, 25, 2163-2175. doi:10.1111/jcmm.16196. https://pubmed.ncbi.nlm.nih.gov/33345387/
5. Huang, Renli, Wu, Cheng, Wen, Jialing, Yu, Jinlong, Zou, Zhaowei. 2022. DIAPH3 is a prognostic biomarker and inhibit colorectal cancer progression through maintaining EGFR degradation. In Cancer medicine, 11, 4688-4702. doi:10.1002/cam4.4793. https://pubmed.ncbi.nlm.nih.gov/35538918/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen