C57BL/6JCya-Fads2em1flox/Cya
Common Name:
Fads2-flox
Product ID:
S-CKO-12125
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Fads2-flox
Strain ID
CKOCMP-56473-Fads2-B6J-VA
Gene Name
Product ID
S-CKO-12125
Gene Alias
2900042M13Rik; Fads2a; Fadsd2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fads2em1flox/Cya mice (Catalog S-CKO-12125) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025567
NCBI RefSeq
NM_019699
Target Region
Exon 1
Size of Effective Region
~4.1 kb
Detailed Document
Overview of Gene Research
Fads2, also known as acyl-CoA 6-desaturase, is a key enzyme in long-chain polyunsaturated fatty acid (LC-PUFA) biosynthesis [5]. It is involved in the desaturation of fatty acids, catalyzing biosynthesis of several unsaturated fatty acids by Δ6, Δ8, and Δ4 desaturation, and is thus crucial for fatty acid metabolism pathways [1]. Fads2's activity has significant implications for various biological processes, and genetic models can be valuable for studying its functions.
Fads2 silencing in MCF7 cells and in vivo breast cancer models causes the synthesis of sciadonic acid, which lacks the internal Δ8 double bond required for the synthesis of prostaglandin and leukotriene, among other eicosanoids [1]. In ascites-derived ovarian cancer cells, genetic ablation of FADS2 retarded tumor growth, cancer stem cell formation, and reduced platinum resistance [2]. In cholangiocarcinoma, FADS2 depletion reduced in vivo tumorigenicity, cell proliferation, migration, and sphere-forming ability, and increased apoptotic cell death and ferroptosis markers [4]. In mouse embryonic fibroblasts, inhibiting mTOR signaling, which is related to FADS2 expression, decreased FADS2 expression and sapienate biosynthesis [3].
In conclusion, Fads2 plays a vital role in fatty acid metabolism. Through gene-knockout and related models, its impact on cancer development and progression has been revealed, such as in breast, ovarian, and cholangiocarcinoma. These studies help us understand the role of Fads2 in disease-related biological processes and may provide potential targets for cancer treatment [1,2,3,4].
References:
1. Kothapalli, Kumar S D, Park, Hui Gyu, Kothapalli, Niharika S L, Brenna, J Thomas. 2023. FADS2 function at the major cancer hotspot 11q13 locus alters fatty acid metabolism in cancer. In Progress in lipid research, 92, 101242. doi:10.1016/j.plipres.2023.101242. https://pubmed.ncbi.nlm.nih.gov/37597812/
2. Xuan, Yang, Wang, Huogang, Yung, Mingo Mh, Chan, Karen Kl, Chan, David W. 2022. SCD1/FADS2 fatty acid desaturases equipoise lipid metabolic activity and redox-driven ferroptosis in ascites-derived ovarian cancer cells. In Theranostics, 12, 3534-3552. doi:10.7150/thno.70194. https://pubmed.ncbi.nlm.nih.gov/35547771/
3. Triki, Mouna, Rinaldi, Gianmarco, Planque, Melanie, Schulze, Almut, Fendt, Sarah-Maria. . mTOR Signaling and SREBP Activity Increase FADS2 Expression and Can Activate Sapienate Biosynthesis. In Cell reports, 31, 107806. doi:10.1016/j.celrep.2020.107806. https://pubmed.ncbi.nlm.nih.gov/32579932/
4. Hasegawa, Kohsei, Fujimori, Haruna, Nakatani, Kohta, Katori, Yukio, Tamai, Keiichi. 2024. Delta-6 desaturase FADS2 is a tumor-promoting factor in cholangiocarcinoma. In Cancer science, 115, 3346-3357. doi:10.1111/cas.16306. https://pubmed.ncbi.nlm.nih.gov/39113435/
5. Bláhová, Zuzana, Harvey, Thomas Nelson, Pšenička, Martin, Mráz, Jan. 2020. Assessment of Fatty Acid Desaturase (Fads2) Structure-Function Properties in Fish in the Context of Environmental Adaptations and as a Target for Genetic Engineering. In Biomolecules, 10, . doi:10.3390/biom10020206. https://pubmed.ncbi.nlm.nih.gov/32023831/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen