C57BL/6JCya-Slc2a5em1flox/Cya
Common Name:
Slc2a5-flox
Product ID:
S-CKO-12128
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Slc2a5-flox
Strain ID
CKOCMP-56485-Slc2a5-B6J-VA
Gene Name
Product ID
S-CKO-12128
Gene Alias
Glut5; Slc5a
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc2a5em1flox/Cya mice (Catalog S-CKO-12128) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030826
NCBI RefSeq
NM_019741
Target Region
Exon 4~5
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Slc2a5, also known as GLUT5, is the major fructose transporter in mammalian cells. It facilitates the uptake of fructose into cells, which is then phosphorylated by ketohexokinase to form fructose 1-phosphate, participating in the fructose metabolism pathway. This process is of significance in various biological aspects, such as nutrient absorption and cell survival [2,4].
Nuclease technology-mediated inactivation of the Slc2a5 gene inhibited cancer cell proliferation, migration in vitro and metastases in vivo in several animal models, uncovering its importance in directing mitochondrial function for cancer cell motility [1]. In lung adenocarcinoma, depletion of Slc2a5 undermined cell proliferation and invasion, while overexpression enhanced these processes [5]. In childhood Philadelphia chromosome-positive acute lymphoblastic leukaemia, tyrosine kinase inhibitors repressed Slc2a5 expression, and fructose protected tumour cells from drug-induced cell death [3]. In tumor endothelial cells, inhibiting Slc2a5 attenuated dietary fructose-enhanced tumor angiogenesis [6].
In conclusion, Slc2a5 plays a crucial role in fructose-mediated processes. Gene-knockout and other loss-of-function models have revealed its significance in cancer-related processes, including metastasis, cell proliferation, and angiogenesis, providing potential therapeutic targets for cancer treatment.
References:
1. Groenendyk, Jody, Stoletov, Konstantin, Paskevicius, Tautvydas, Agellon, Luis B, Michalak, Marek. 2022. Loss of the fructose transporter SLC2A5 inhibits cancer cell migration. In Frontiers in cell and developmental biology, 10, 896297. doi:10.3389/fcell.2022.896297. https://pubmed.ncbi.nlm.nih.gov/36268513/
2. Taylor, Samuel R, Ramsamooj, Shakti, Liang, Roger J, Rhee, Kyu Y, Goncalves, Marcus D. 2021. Dietary fructose improves intestinal cell survival and nutrient absorption. In Nature, 597, 263-267. doi:10.1038/s41586-021-03827-2. https://pubmed.ncbi.nlm.nih.gov/34408323/
3. Zhao, Pan, Huang, Jingcao, Zhang, Dan, Niu, Ting, Zheng, Yuhuan. 2018. SLC2A5 overexpression in childhood philadelphia chromosome-positive acute lymphoblastic leukaemia. In British journal of haematology, 183, 242-250. doi:10.1111/bjh.15580. https://pubmed.ncbi.nlm.nih.gov/30272826/
4. Liang, Roger J, Taylor, Samuel, Nahiyaan, Navid, Rhee, Kyu Y, Goncalves, Marcus D. 2021. GLUT5 (SLC2A5) enables fructose-mediated proliferation independent of ketohexokinase. In Cancer & metabolism, 9, 12. doi:10.1186/s40170-021-00246-9. https://pubmed.ncbi.nlm.nih.gov/33762003/
5. Weng, Yuanyuan, Fan, Xueyu, Bai, Yongfeng, Zhu, Jin, Zhang, Feng. 2018. SLC2A5 promotes lung adenocarcinoma cell growth and metastasis by enhancing fructose utilization. In Cell death discovery, 4, 38. doi:10.1038/s41420-018-0038-5. https://pubmed.ncbi.nlm.nih.gov/29531835/
6. Fang, Jian-Hong, Chen, Jie-Ying, Zheng, Jia-Lin, Wang, Zhi-Yong, Zhuang, Shi-Mei. . Fructose Metabolism in Tumor Endothelial Cells Promotes Angiogenesis by Activating AMPK Signaling and Mitochondrial Respiration. In Cancer research, 83, 1249-1263. doi:10.1158/0008-5472.CAN-22-1844. https://pubmed.ncbi.nlm.nih.gov/36715635/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen