C57BL/6JCya-Gabarapem1flox/Cya
Common Name:
Gabarap-flox
Product ID:
S-CKO-12129
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gabarap-flox
Strain ID
CKOCMP-56486-Gabarap-B6J-VA
Gene Name
Product ID
S-CKO-12129
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gabarapem1flox/Cya mice (Catalog S-CKO-12129) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000018711
NCBI RefSeq
NM_019749
Target Region
Exon 2~3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
GABARAP, short for GABA type A receptor-associated protein, is a member of the LC3/GABARAP protein family which evolved from yeast Atg8. It plays crucial roles in autophagy, a cellular process for degrading and recycling aged and cytotoxic components. Autophagy involves cargo capture in autophagosomes and subsequent lysosomal fusion. GABARAP is also implicated in processes like mitophagy, a form of selective autophagy, and is important for maintaining cellular homeostasis [1,2].
In multiple myeloma, loss-of-function studies have shown that deletion of GABARAP, which is commonly deleted on chromosome 17p in high-risk patients, impairs the exposure of the eat-me signal CRT on dying tumor cells. This reduces tumor cell phagocytosis by dendritic cells and the subsequent antitumor T-cell response, leading to resistance to immunogenic chemotherapy like bortezomib treatment. Mechanistically, GABARAP deletion blocks ICD signaling by decreasing autophagy and altering Golgi apparatus morphology [3].
In conclusion, GABARAP is essential in autophagy-related processes and has significant implications in cancer, particularly in multiple myeloma where its deletion is associated with poor outcomes. The study of GABARAP knockout models has provided valuable insights into its role in tumor cell immunogenicity and response to chemotherapy, highlighting its potential as a therapeutic target in this disease [3].
References:
1. Schaaf, Marco B E, Keulers, Tom G, Vooijs, Marc A, Rouschop, Kasper M A. 2016. LC3/GABARAP family proteins: autophagy-(un)related functions. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 30, 3961-3978. doi:. https://pubmed.ncbi.nlm.nih.gov/27601442/
2. Le Guerroué, François, Bunker, Eric N, Rosencrans, William M, Wang, Chunxin, Youle, Richard J. 2023. TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1. In Molecular cell, 83, 927-941.e8. doi:10.1016/j.molcel.2023.02.023. https://pubmed.ncbi.nlm.nih.gov/36898370/
3. Gulla, Annamaria, Morelli, Eugenio, Johnstone, Megan, Munshi, Nikhil C, Anderson, Kenneth C. . Loss of GABARAP mediates resistance to immunogenic chemotherapy in multiple myeloma. In Blood, 143, 2612-2626. doi:10.1182/blood.2023022777. https://pubmed.ncbi.nlm.nih.gov/38551812/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen