C57BL/6JCya-Nxt1em1flox/Cya
Common Name:
Nxt1-flox
Product ID:
S-CKO-12130
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nxt1-flox
Strain ID
CKOCMP-56488-Nxt1-B6J-VA
Gene Name
Product ID
S-CKO-12130
Gene Alias
1110001N02Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nxt1em1flox/Cya mice (Catalog S-CKO-12130) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000109961
NCBI RefSeq
NM_001110159
Target Region
Exon 3
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Nxt1, also known as p15 or p15-1, is an NTF2-like export protein crucial for RNA translocation across the nuclear envelope [1,2]. It shuttles between the nucleus and cytoplasm in metazoan cells and is involved in the nuclear export of diverse RNAs, playing a vital role in mRNA export pathways essential for protein production and normal cellular functions [1,5,6]. Genetic models, such as those in primates and murine rodents, are valuable for studying Nxt1 [1].
In Drosophila, a partial loss-of-function allele of Nxt1 leads to reduced viability, sterility, and muscle degeneration, along with abnormal mRNA and circRNA expression [5]. In mammalian cells, Nxt1 is a crucial cofactor in TAP-dependent export of intron-containing RNA, and its binding to Tap enhances the recruitment of the complex to nuclear pore complexes for mRNA export [7,8]. Also, Nxt1 promotes the nuclear export of influenza virus nucleoprotein via a CRM1-dependent pathway, and its knockdown decreases viral replication [3]. In SARS-CoV-2 infection, the virus's Nsp1 targets the NXF1-Nxt1 mRNA export receptor, and inhibition of this export promotes pathogenesis [4].
In conclusion, Nxt1 is essential for RNA nuclear export, affecting various biological processes. Studies using gene-knockout or similar models in different organisms have revealed its roles in muscle integrity, viral replication, and host-pathogen interactions. Understanding Nxt1's functions provides insights into normal biological mechanisms and potential therapeutic targets for viral diseases [1,3,4,5,7,8].
References:
1. Hu, Jia, Wang, Hong, He, Dan, Lyu, Yongqing, Li, Yan. 2022. Genetic characterization of nuclear export factor NXT1 and its paralog NXT2 in primates and murine rodents. In Zoology (Jena, Germany), 151, 126002. doi:10.1016/j.zool.2022.126002. https://pubmed.ncbi.nlm.nih.gov/35219094/
2. Black, B E, Holaska, J M, Lévesque, L, Dargemont, C, Paschal, B M. . NXT1 is necessary for the terminal step of Crm1-mediated nuclear export. In The Journal of cell biology, 152, 141-55. doi:. https://pubmed.ncbi.nlm.nih.gov/11149927/
3. Chutiwitoonchai, Nopporn, Aida, Yoko. 2016. NXT1, a Novel Influenza A NP Binding Protein, Promotes the Nuclear Export of NP via a CRM1-Dependent Pathway. In Viruses, 8, . doi:10.3390/v8080209. https://pubmed.ncbi.nlm.nih.gov/27483302/
4. Mei, Menghan, Cupic, Anastasija, Miorin, Lisa, García-Sastre, Adolfo, Fontoura, Beatriz M A. 2024. Inhibition of mRNA nuclear export promotes SARS-CoV-2 pathogenesis. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2314166121. doi:10.1073/pnas.2314166121. https://pubmed.ncbi.nlm.nih.gov/38768348/
5. van der Graaf, Kevin, Jindrich, Katia, Mitchell, Robert, White-Cooper, Helen. . Roles for RNA export factor, Nxt1, in ensuring muscle integrity and normal RNA expression in Drosophila. In G3 (Bethesda, Md.), 11, . doi:10.1093/g3journal/jkaa046. https://pubmed.ncbi.nlm.nih.gov/33561245/
6. Caporilli, Simona, Yu, Yachuan, Jiang, Jianqiao, White-Cooper, Helen. 2013. The RNA export factor, Nxt1, is required for tissue specific transcriptional regulation. In PLoS genetics, 9, e1003526. doi:10.1371/journal.pgen.1003526. https://pubmed.ncbi.nlm.nih.gov/23754955/
7. Guzik, B W, Levesque, L, Prasad, S, Rekosh, D, Hammarskjöld, M L. . NXT1 (p15) is a crucial cellular cofactor in TAP-dependent export of intron-containing RNA in mammalian cells. In Molecular and cellular biology, 21, 2545-54. doi:. https://pubmed.ncbi.nlm.nih.gov/11259602/
8. Wiegand, Heather L, Coburn, Glen A, Zeng, Yan, Bogerd, Hal P, Cullen, Bryan R. . Formation of Tap/NXT1 heterodimers activates Tap-dependent nuclear mRNA export by enhancing recruitment to nuclear pore complexes. In Molecular and cellular biology, 22, 245-56. doi:. https://pubmed.ncbi.nlm.nih.gov/11739738/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen