C57BL/6JCya-Clec4nem1flox/Cya
Common Name
Clec4n-flox
Product ID
S-CKO-12173
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-56620-Clec4n-B6J-VA
When using this mouse strain in a publication, please cite “Clec4n-flox Mouse (Catalog S-CKO-12173) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Clec4n-flox
Strain ID
CKOCMP-56620-Clec4n-B6J-VA
Gene Name
Product ID
S-CKO-12173
Gene Alias
Clec6a, Clecsf10, Nkcl
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000024118
NCBI RefSeq
NM_020001
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Overview of Gene Research
Clec4n, also known as Dectin-2, is a C-type lectin receptor. It functions as a pattern-recognition receptor for fungi and some components of house dust mite (HDM) extract. It is involved in the activation of signaling pathways such as Syk-NF-κB and Syk-JNK, and plays a role in immune responses, including Th2 and Th17 cell differentiation [1,4].
In Clec4n-deficient (Clec4n(-/-)) mice, HDM-induced allergic airway inflammation was significantly attenuated, with decreased Th2 and Th17 cell differentiation [4]. Also, Clec4n -/- mice had a decreased Th2 immune response following HDM challenge due to reduced type 2 conventional dendritic cells (cDC2s) in the lung [1]. In the context of mycobacterial infection, Clec4n(-/-) mice showed augmented lung pathology as Dectin-2 (Clec4n) contributes to host immunity against mycobacterial infection through the recognition of mannose-capped lipoarabinomannan (Man-LAM) [2]. In a study on epidermolysis bullosa acquisita, although Clec4n was upregulated in lesional skin, deficiency in Clec4n in neutrophil-specific Clec4n -/- mice did not reduce disease in the EBA model [3].
In conclusion, Clec4n, as Dectin-2, is crucial in immune responses, especially in those related to fungal recognition and allergic airway inflammation. Gene-knockout mouse models have been instrumental in revealing its role in these processes, highlighting its potential as a target for treating diseases such as asthma and mycobacterial infections.
References：
1. Qu, Yulan, Ma, Xiaotian, Deng, Jiewen, Guo, Zhenhong, Bai, Chong. 2022. Dectin-2 promotes house dust mite-skewed Th2 response through the activation of cDC2s. In Cellular immunology, 378, 104558. doi:10.1016/j.cellimm.2022.104558. https://pubmed.ncbi.nlm.nih.gov/35717749/
2. Yonekawa, Akiko, Saijo, Shinobu, Hoshino, Yoshihiko, Akashi, Koichi, Yamasaki, Sho. 2014. Dectin-2 is a direct receptor for mannose-capped lipoarabinomannan of mycobacteria. In Immunity, 41, 402-413. doi:10.1016/j.immuni.2014.08.005. https://pubmed.ncbi.nlm.nih.gov/25176311/
3. Guerrero-Juarez, Christian F, Schilf, Paul, Li, Jing, Sadik, Christian D, Amber, Kyle T. 2023. C-type lectin receptor expression is a hallmark of neutrophils infiltrating the skin in epidermolysis bullosa acquisita. In Frontiers in immunology, 14, 1266359. doi:10.3389/fimmu.2023.1266359. https://pubmed.ncbi.nlm.nih.gov/37799716/
4. Norimoto, Ayako, Hirose, Koichi, Iwata, Arifumi, Iwakura, Yoichiro, Nakajima, Hiroshi. . Dectin-2 promotes house dust mite-induced T helper type 2 and type 17 cell differentiation and allergic airway inflammation in mice. In American journal of respiratory cell and molecular biology, 51, 201-9. doi:10.1165/rcmb.2013-0522OC. https://pubmed.ncbi.nlm.nih.gov/24588637/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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