C57BL/6JCya-Ccnl1em1flox/Cya
Common Name:
Ccnl1-flox
Product ID:
S-CKO-12200
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ccnl1-flox
Strain ID
CKOCMP-56706-Ccnl1-B6J-VA
Gene Name
Product ID
S-CKO-12200
Gene Alias
2610030E23Rik; Ccnl; ania-6a
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccnl1em1flox/Cya mice (Catalog S-CKO-12200) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029416
NCBI RefSeq
NM_019937
Target Region
Exon 4
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Ccnl1, also known as Cyclin L1, is a non-canonical cyclin. It is involved in multiple biological processes. Functionally, it participates in pre-mRNA splicing activities as it interacts with TFIP11 and EWSR1 which are related to the spliceosome [5]. It also plays a role in cell cycle regulation, specifically at the G2-M phase as part of the CCNL1-CDK11 complex [1].
In cancer-related research, loss-of-function experiments have shown significant results. In pancreatic cancer, knockout of Ccnl1 enhanced gemcitabine resistance by activating the ERK/AKT/STAT3 survival pathway [4]. In prostate cancer, miR-5195-3p, which targets Ccnl1, acts as a tumor suppressor, with overexpression of miR-5195-3p suppressing cell proliferation and cell cycle G1/S transition [2]. In osteosarcoma, Ccnl1 promotes cell proliferation, migration, and Adriamycin resistance through the PI3K/AKT-mTOR pathway [3].
In conclusion, Ccnl1 is crucial for pre-mRNA splicing and cell cycle regulation. Its role in cancer-related processes, such as drug resistance and tumor cell proliferation in pancreatic, prostate, and osteosarcoma cancers, has been revealed through loss-of-function studies. Understanding Ccnl1 can provide potential targets for cancer treatment.
References：
1. O'Brien, Siobhan, Kelso, Susan, Steinhart, Zachary, Sicheri, Frank, Angers, Stephane. 2022. SCFFBXW7 regulates G2-M progression through control of CCNL1 ubiquitination. In EMBO reports, 23, e55044. doi:10.15252/embr.202255044. https://pubmed.ncbi.nlm.nih.gov/36278408/
2. Zeng, Xing, Hu, Zhiquan, Shen, Yuanqing, Gan, Jiahua, Liu, Zheng. 2022. MiR-5195-3p functions as a tumor suppressor in prostate cancer via targeting CCNL1. In Cellular & molecular biology letters, 27, 25. doi:10.1186/s11658-022-00326-8. https://pubmed.ncbi.nlm.nih.gov/35260070/
3. Zhang, Yanbin, Zhang, Tao, Chen, Long, Guo, Zijun, Jiang, Xiaobing. 2024. Cyclin L1 participates in Adriamycin resistance and progression of osteosarcoma via PI3K/AKT-mTOR pathway. In Aging, 16, 11208-11223. doi:10.18632/aging.205972. https://pubmed.ncbi.nlm.nih.gov/39024509/
4. Yang, Hai, Liu, Bin, Liu, Dongxue, Grützmann, Robert, Pilarsky, Christian. 2022. Genome-Wide CRISPR Screening Identifies DCK and CCNL1 as Genes That Contribute to Gemcitabine Resistance in Pancreatic Cancer. In Cancers, 14, . doi:10.3390/cancers14133152. https://pubmed.ncbi.nlm.nih.gov/35804923/
5. Tannukit, Sissada, Wen, Xin, Wang, HongJun, Paine, Michael L. 2008. TFIP11, CCNL1 and EWSR1 Protein-protein Interactions, and Their Nuclear Localization. In International journal of molecular sciences, 9, 1504-1514. doi:10.3390/ijms9081504. https://pubmed.ncbi.nlm.nih.gov/19122807/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen