C57BL/6JCya-Clec1bem1flox/Cya
Common Name:
Clec1b-flox
Product ID:
S-CKO-12232
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Clec1b-flox
Strain ID
CKOCMP-56760-Clec1b-B6J-VA
Gene Name
Product ID
S-CKO-12232
Gene Alias
1810061I13Rik; Clec-2; Clec2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Clec1bem1flox/Cya mice (Catalog S-CKO-12232) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032262
NCBI RefSeq
NM_019985
Target Region
Exon 2~4
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Clec1b, also known as the gene encoding the CLEC-2 protein, is a member of the C-type lectin superfamily. It is a type II transmembrane receptor involved in platelet activation, angiogenesis, immune and inflammatory responses, tumor cell metastasis, separation of blood/lymphatic vessels, and cerebrovascular patterning during embryonic development [1,2,3]. It binds to ligands like podoplanin and is expressed on platelets, Kupffer cells, and other immune cells [3].
Using constitutive Clec1b(-/-) mice, it was shown that CLEC-2 was not necessary for initiation of the lymph node anlage but required at late stages of development. Constitutive deletion induced a profound defect in lymphatic endothelial cell proliferation, resulting in lack of lymph nodes at birth. Conditional deletion of CLEC-2 in the megakaryocyte/platelet lineage in Clec1b(fl/fl)PF4-Cre mice led to the development of blood-filled lymph nodes and fibrosis, indicating CLEC-2 expression in platelets was required for lymph node integrity [4].
In conclusion, Clec1b plays essential roles in multiple biological processes. Its function in platelet-related processes and lymph node development has been well-demonstrated through gene knockout mouse models. Additionally, studies in hepatocellular carcinoma suggest its potential as a prognostic biomarker and immunoregulator, highlighting its significance in disease-related research [1-3, 9].
References:
1. Jing, Qiangan, Yuan, Chen, Zhou, Chaoting, Li, Yanchun, Shao, Fangchun. 2023. Comprehensive analysis identifies CLEC1B as a potential prognostic biomarker in hepatocellular carcinoma. In Cancer cell international, 23, 113. doi:10.1186/s12935-023-02939-1. https://pubmed.ncbi.nlm.nih.gov/37308868/
2. Liang, Xiaoliang, Song, Fei, Fang, Wanzhi, Han, Lu, Chen, Zhong. 2022. CLEC1B is a Promising Prognostic Biomarker and Correlated with Immune Infiltration in Hepatocellular Carcinoma. In International journal of general medicine, 15, 5661-5672. doi:10.2147/IJGM.S363050. https://pubmed.ncbi.nlm.nih.gov/35734199/
3. Meng, Danyang, Luo, Man, Liu, Beibei. 2021. The Role of CLEC-2 and Its Ligands in Thromboinflammation. In Frontiers in immunology, 12, 688643. doi:10.3389/fimmu.2021.688643. https://pubmed.ncbi.nlm.nih.gov/34177942/
4. Bénézech, Cécile, Nayar, Saba, Finney, Brenda A, Buckley, Christopher D, Barone, Francesca. 2014. CLEC-2 is required for development and maintenance of lymph nodes. In Blood, 123, 3200-7. doi:10.1182/blood-2013-03-489286. https://pubmed.ncbi.nlm.nih.gov/24532804/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen