C57BL/6JCya-Scamp5em1flox/Cya
Common Name:
Scamp5-flox
Product ID:
S-CKO-12245
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Scamp5-flox
Strain ID
CKOCMP-56807-Scamp5-B6J-VA
Gene Name
Product ID
S-CKO-12245
Gene Alias
Sc5
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Scamp5em1flox/Cya mice (Catalog S-CKO-12245) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000046587
NCBI RefSeq
NM_020270
Target Region
Exon 4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Scamp5, a member of the secretory carrier membrane protein family, is highly expressed in the brain. It participates in membrane transport, regulating exocytosis and endocytosis of synaptic vesicles (SVs), and is crucial for maintaining normal nerve cell physiological functions. It is also related to secretion carrier and membrane function [1].
In genetic models, Scamp5-deficient models have provided insights. For instance, Scamp5 R91W mutant knock-in mice show early-onset epilepsy similar to human pediatric epilepsy cases. The mutation affects the interaction between Scamp5 and synaptotagmin 1, dysregulating neurotransmitter release and shifting the excitation/inhibition balance in the neuronal network [3]. Additionally, knockdown of Scamp5 in cultured rat hippocampal neurons reduces the total and recycling vesicle pool sizes, slows endocytosis, and impairs it during strong stimulation, suggesting its role in high neuronal activity [2].
In conclusion, Scamp5 is essential for synaptic function, with its disruption leading to neurological disorders such as epilepsy. Mouse models, especially those with loss-of-function mutations or knockdown, have been instrumental in revealing its role in regulating neurotransmitter release and synaptic vesicle dynamics, providing a better understanding of the pathogenesis of related neurological diseases.
References:
1. Chen, Ye, Fan, Jiali, Xiao, Dongqiong, Li, Xihong. 2022. The role of SCAMP5 in central nervous system diseases. In Neurological research, 44, 1024-1037. doi:10.1080/01616412.2022.2107754. https://pubmed.ncbi.nlm.nih.gov/36217917/
2. Zhao, Haiyan, Kim, Yoonju, Park, Joohyun, Chang, Iree, Chang, Sunghoe. . SCAMP5 plays a critical role in synaptic vesicle endocytosis during high neuronal activity. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 34, 10085-95. doi:10.1523/JNEUROSCI.2156-14.2014. https://pubmed.ncbi.nlm.nih.gov/25057210/
3. Zhang, Dazhi, Yuan, Chao, Liu, Mengxue, Yang, Xiaofei, Zhang, Xianqin. 2020. Deficiency of SCAMP5 leads to pediatric epilepsy and dysregulation of neurotransmitter release in the brain. In Human genetics, 139, 545-555. doi:10.1007/s00439-020-02123-9. https://pubmed.ncbi.nlm.nih.gov/32020363/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen