C57BL/6NCya-Ltb4r2em1flox/Cya
Common Name:
Ltb4r2-flox
Product ID:
S-CKO-12282
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ltb4r2-flox
Strain ID
CKOCMP-57260-Ltb4r2-B6N-VA
Gene Name
Product ID
S-CKO-12282
Gene Alias
5830462O07Rik; BLT2; LTB4-R 2; LTB4-R-2; LTB4-R2
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ltb4r2em1flox/Cya mice (Catalog S-CKO-12282) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000044554
NCBI RefSeq
NM_020490
Target Region
Exon 2
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Ltb4r2, also known as BLT2, is a low-affinity receptor for several key eicosanoids and chemoattractants. It is involved in multiple biological processes related to inflammation and cancer. Leukotrienes, the ligands of Ltb4r2, are arachidonic acid-derived bioactive molecules known as mediators of allergic and inflammatory reactions [2].
In liver tumorigenesis, selective ablation of Scd2 in activated hepatic stellate cells (aHSC) globally suppresses nuclear CTNNB1 and YAP1 in tumors and the tumor microenvironment, and prevents liver tumorigenesis in male mice. This is associated with reduced Ltb4r2 and its ligand 12-HHTrE. Genetic or pharmacological inhibition of Ltb4r2 recapitulates CTNNB1 and YAP1 inactivation and tumor suppression in culture and in vivo, suggesting that the 12-HHTrE-Ltb4r2-CTNNB1-YAP1 pathway initiated by aHSC is a potential HCC therapeutic target [1].
In the context of tissue inflammation, studies using Ltb4r2-/-mice and zebrafish models with blt2a knockdown or chemical inhibition of BLT2 signaling show that Ltb4r2 is required for macrophage chemotactic function during inflammation, implicating it as a potential therapeutic target for inflammatory pathologies [3].
In conclusion, Ltb4r2 plays crucial roles in inflammation-related macrophage migration and in promoting liver tumorigenesis through the activation of specific signaling cascades. The use of gene-knockout mouse models and other loss-of-function experiments in these studies has revealed its significance in these biological processes and disease conditions, highlighting its potential as a therapeutic target for liver cancer and inflammatory pathologies.
References:
1. Sinha, Sonal, Aizawa, Satoka, Nakano, Yasuhiro, Guccione, Ernesto, Tsukamoto, Hidekazu. 2023. Hepatic stellate cell stearoyl co-A desaturase activates leukotriene B4 receptor 2 - β-catenin cascade to promote liver tumorigenesis. In Nature communications, 14, 2651. doi:10.1038/s41467-023-38406-8. https://pubmed.ncbi.nlm.nih.gov/37156770/
2. Yokomizo, Takehiko, Nakamura, Motonao, Shimizu, Takao. 2018. Leukotriene receptors as potential therapeutic targets. In The Journal of clinical investigation, 128, 2691-2701. doi:10.1172/JCI97946. https://pubmed.ncbi.nlm.nih.gov/29757196/
3. Ermis, Ebru, Nargis, Titli, Webster, Kierstin, Anderson, Ryan M, Mirmira, Raghavendra G. 2023. Leukotriene B4 receptor 2 governs macrophage migration during tissue inflammation. In The Journal of biological chemistry, 300, 105561. doi:10.1016/j.jbc.2023.105561. https://pubmed.ncbi.nlm.nih.gov/38097183/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen