C57BL/6NCya-Brd4em1flox/Cya
Common Name:
Brd4-flox
Product ID:
S-CKO-12283
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Brd4-flox
Strain ID
CKOCMP-57261-Brd4-B6N-VA
Gene Name
Product ID
S-CKO-12283
Gene Alias
Brd5; HUNK1; MCAP; WI-11513
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Brd4em1flox/Cya mice (Catalog S-CKO-12283) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003726
NCBI RefSeq
NM_001286630
Target Region
Exon 4~5
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Brd4, a member of the Bromodomain and Extraterminal (BET) protein family, is an epigenetic reader that binds to acetylated histone marks. It plays pleiotropic roles in regulating gene transcription, especially in the organization of super-enhancers (SEs) and the expression of oncogenes. Additionally, Brd4 is involved in maintaining genome stability, controlling DNA damage checkpoint activation, repair, and telomere maintenance [1]. It also participates in multiple cellular processes relevant to various diseases, such as cell proliferation, apoptosis, and different forms of programmed cell death [7].
In cancer, inhibition of Brd4 can disrupt the communication between SEs and target promoters, leading to cell-specific repression of oncogenes and subsequent cell death [1]. For example, in glioblastoma, several Brd4 inhibitors and degraders have shown promise in suppressing the disease, though the exact role and mechanism in its pathogenesis are yet to be fully elucidated [2]. In NUT carcinoma, the BRD4-NUT fusion oncoprotein drives the disease, potentially acting through the formation of massive super-enhancers [4]. In acute gouty arthritis, targeting Brd4 can prevent the disease by regulating pyroptosis [3]. Also, Brd4 is involved in the life-cycle of human papillomaviruses and is a potential target for associated cancers [5]. In lung diseases, Brd4 has been identified as a potential therapeutic target [6].
In conclusion, Brd4 is a crucial protein involved in gene transcription regulation and genome stability maintenance. Its role in various diseases, especially cancer, has been well-studied through in vivo and in vitro experiments. Research on Brd4, including gene knockout (KO) or conditional knockout (CKO) mouse models, has provided insights into its functions in specific biological processes and disease conditions, highlighting its potential as a therapeutic target.
References:
1. Donati, Benedetta, Lorenzini, Eugenia, Ciarrocchi, Alessia. 2018. BRD4 and Cancer: going beyond transcriptional regulation. In Molecular cancer, 17, 164. doi:10.1186/s12943-018-0915-9. https://pubmed.ncbi.nlm.nih.gov/30466442/
2. Duan, Weichen, Yu, Miao, Chen, Jiajia. 2023. BRD4: New hope in the battle against glioblastoma. In Pharmacological research, 191, 106767. doi:10.1016/j.phrs.2023.106767. https://pubmed.ncbi.nlm.nih.gov/37061146/
3. Hao, Kun, Jiang, Wenjiao, Zhou, Mengze, Jiang, Fei, Hu, Qinghua. 2020. Targeting BRD4 prevents acute gouty arthritis by regulating pyroptosis. In International journal of biological sciences, 16, 3163-3173. doi:10.7150/ijbs.46153. https://pubmed.ncbi.nlm.nih.gov/33162822/
4. Eagen, Kyle P, French, Christopher A. 2021. Supercharging BRD4 with NUT in carcinoma. In Oncogene, 40, 1396-1408. doi:10.1038/s41388-020-01625-0. https://pubmed.ncbi.nlm.nih.gov/33452461/
5. Rani, Abdul Qawee, Bonam, Srinivasa Reddy, Zhou, Jia, Hu, Haitao, Liu, Xuefeng. . BRD4 as a potential target for human papillomaviruses associated cancer. In Journal of medical virology, 95, e29294. doi:10.1002/jmv.29294. https://pubmed.ncbi.nlm.nih.gov/38100650/
6. Guo, Xia, Olajuyin, Ayobami, Tucker, Torry A, Idell, Steven, Qian, Guoqing. 2023. BRD4 as a Therapeutic Target in Pulmonary Diseases. In International journal of molecular sciences, 24, . doi:10.3390/ijms241713231. https://pubmed.ncbi.nlm.nih.gov/37686037/
7. Hu, Jinfeng, Pan, Dun, Li, Guo, Chen, Kunqi, Hu, Xiangming. 2022. Regulation of programmed cell death by Brd4. In Cell death & disease, 13, 1059. doi:10.1038/s41419-022-05505-1. https://pubmed.ncbi.nlm.nih.gov/36539410/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen