Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6NCya-Brd4em1flox/Cya
Common Name:
Brd4-flox
Product ID:
S-CKO-12283
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Brd4-flox
Strain ID
CKOCMP-57261-Brd4-B6N-VA
Gene Name
Brd4
Product ID
S-CKO-12283
Gene Alias
Brd5; HUNK1; MCAP; WI-11513
Background
C57BL/6NCya
NCBI ID
57261
Modification
Conditional knockout
Chromosome
17
Phenotype
MGI:1888520
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Brd4em1flox/Cya mice (Catalog S-CKO-12283) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003726
NCBI RefSeq
NM_001286630
Target Region
Exon 4~5
Size of Effective Region
~2.1 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Brd4, a member of the Bromodomain and Extraterminal (BET) protein family, is an epigenetic reader that binds to acetylated histone marks. It plays pleiotropic roles in regulating gene transcription, especially in the organization of super-enhancers (SEs) and the expression of oncogenes. Additionally, Brd4 is involved in maintaining genome stability, controlling DNA damage checkpoint activation, repair, and telomere maintenance [1]. It also participates in multiple cellular processes relevant to various diseases, such as cell proliferation, apoptosis, and different forms of programmed cell death [7].

In cancer, inhibition of Brd4 can disrupt the communication between SEs and target promoters, leading to cell-specific repression of oncogenes and subsequent cell death [1]. For example, in glioblastoma, several Brd4 inhibitors and degraders have shown promise in suppressing the disease, though the exact role and mechanism in its pathogenesis are yet to be fully elucidated [2]. In NUT carcinoma, the BRD4-NUT fusion oncoprotein drives the disease, potentially acting through the formation of massive super-enhancers [4]. In acute gouty arthritis, targeting Brd4 can prevent the disease by regulating pyroptosis [3]. Also, Brd4 is involved in the life-cycle of human papillomaviruses and is a potential target for associated cancers [5]. In lung diseases, Brd4 has been identified as a potential therapeutic target [6].

In conclusion, Brd4 is a crucial protein involved in gene transcription regulation and genome stability maintenance. Its role in various diseases, especially cancer, has been well-studied through in vivo and in vitro experiments. Research on Brd4, including gene knockout (KO) or conditional knockout (CKO) mouse models, has provided insights into its functions in specific biological processes and disease conditions, highlighting its potential as a therapeutic target.

References:
1. Donati, Benedetta, Lorenzini, Eugenia, Ciarrocchi, Alessia. 2018. BRD4 and Cancer: going beyond transcriptional regulation. In Molecular cancer, 17, 164. doi:10.1186/s12943-018-0915-9. https://pubmed.ncbi.nlm.nih.gov/30466442/
2. Duan, Weichen, Yu, Miao, Chen, Jiajia. 2023. BRD4: New hope in the battle against glioblastoma. In Pharmacological research, 191, 106767. doi:10.1016/j.phrs.2023.106767. https://pubmed.ncbi.nlm.nih.gov/37061146/
3. Hao, Kun, Jiang, Wenjiao, Zhou, Mengze, Jiang, Fei, Hu, Qinghua. 2020. Targeting BRD4 prevents acute gouty arthritis by regulating pyroptosis. In International journal of biological sciences, 16, 3163-3173. doi:10.7150/ijbs.46153. https://pubmed.ncbi.nlm.nih.gov/33162822/
4. Eagen, Kyle P, French, Christopher A. 2021. Supercharging BRD4 with NUT in carcinoma. In Oncogene, 40, 1396-1408. doi:10.1038/s41388-020-01625-0. https://pubmed.ncbi.nlm.nih.gov/33452461/
5. Rani, Abdul Qawee, Bonam, Srinivasa Reddy, Zhou, Jia, Hu, Haitao, Liu, Xuefeng. . BRD4 as a potential target for human papillomaviruses associated cancer. In Journal of medical virology, 95, e29294. doi:10.1002/jmv.29294. https://pubmed.ncbi.nlm.nih.gov/38100650/
6. Guo, Xia, Olajuyin, Ayobami, Tucker, Torry A, Idell, Steven, Qian, Guoqing. 2023. BRD4 as a Therapeutic Target in Pulmonary Diseases. In International journal of molecular sciences, 24, . doi:10.3390/ijms241713231. https://pubmed.ncbi.nlm.nih.gov/37686037/
7. Hu, Jinfeng, Pan, Dun, Li, Guo, Chen, Kunqi, Hu, Xiangming. 2022. Regulation of programmed cell death by Brd4. In Cell death & disease, 13, 1059. doi:10.1038/s41419-022-05505-1. https://pubmed.ncbi.nlm.nih.gov/36539410/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest