C57BL/6JCya-As3mtem1flox/Cya
Common Name:
As3mt-flox
Product ID:
S-CKO-12311
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
As3mt-flox
Strain ID
CKOCMP-57344-As3mt-B6J-VA
Gene Name
Product ID
S-CKO-12311
Gene Alias
2310045H08Rik; Cyt19
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-As3mtem1flox/Cya mice (Catalog S-CKO-12311) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003655
NCBI RefSeq
NM_020577
Target Region
Exon 4
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
As3mt, also known as arsenic (3) methyltransferase, is a key enzyme involved in the methylation metabolism of arsenic [3]. It catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (SAM) to trivalent arsenical, playing a role in arsenic biotransformation pathways [2,4]. This process is crucial as arsenic exposure is linked to various health issues, making As3mt an important factor in understanding arsenic-related biological impacts. Genetic models, such as humanized mouse models, can help in studying its functions [6].
In arsenic-induced non-alcoholic fatty liver disease (NAFLD), As3mt via consuming SAM blocks m6A-mediated miR-142-5p maturation, leading to elevated levels of SREBP1 and lipogenic genes, and ultimately causing liver steatosis. SAM supplementation or As3mt deficiency can block arsenic-induced lipid accumulation by promoting miR-142-5p maturation [1]. In arsenic-exposed heterozygous As3mt mice, there is low liver lipid accumulation, indicating the role of As3mt in arsenic-induced NAFLD [1]. Additionally, in arsenic-induced hepatic insulin resistance, AS3MT interacts with NLRP3 to activate the inflammasome and strengthens the m6A methylase association with NLRP3 to stabilize m6A-modified NLRP3, contributing to arsenic-induced hepatic insulin resistance [5].
In conclusion, As3mt is essential in arsenic metabolism. Studies using As3mt-related mouse models have revealed its role in diseases like NAFLD and arsenic-induced hepatic insulin resistance. Understanding As3mt's functions helps in uncovering the mechanisms of arsenic-related diseases, potentially providing new strategies for prevention and treatment.
References：
1. Li, Han, Wu, Lu, Ye, Fuping, Zhang, Aihua, Liu, Qizhan. 2023. As3MT via consuming SAM is involved in arsenic-induced nonalcoholic fatty liver disease by blocking m6A-mediated miR-142-5p maturation. In The Science of the total environment, 892, 164746. doi:10.1016/j.scitotenv.2023.164746. https://pubmed.ncbi.nlm.nih.gov/37301390/
2. Mersaoui, Sofiane Y, Guilbert, Cynthia, Chou, Hsiang, Richard, Stéphane, Mann, Koren K. 2022. Arsenic 3 methyltransferase (AS3MT) automethylates on cysteine residues in vitro. In Archives of toxicology, 96, 1371-1386. doi:10.1007/s00204-022-03248-8. https://pubmed.ncbi.nlm.nih.gov/35244730/
3. Wen, Weihua, Zha, Shun, Cheng, Huirong, Chen, Qian, Gu, Yun. 2023. As3MT is related to relative RNAs and base modifications of p53 in workers exposed to arsenic. In Environmental science and pollution research international, 30, 62094-62103. doi:10.1007/s11356-023-26457-9. https://pubmed.ncbi.nlm.nih.gov/36940027/
4. Agusa, Tetsuro, Fujihara, Junko, Takeshita, Haruo, Iwata, Hisato. 2011. Individual variations in inorganic arsenic metabolism associated with AS3MT genetic polymorphisms. In International journal of molecular sciences, 12, 2351-82. doi:10.3390/ijms12042351. https://pubmed.ncbi.nlm.nih.gov/21731446/
5. Qiu, Tianming, Wu, Chenbing, Yao, Xiaofeng, Yang, Guang, Sun, Xiance. 2022. AS3MT facilitates NLRP3 inflammasome activation by m6A modification during arsenic-induced hepatic insulin resistance. In Cell biology and toxicology, 39, 2165-2181. doi:10.1007/s10565-022-09703-7. https://pubmed.ncbi.nlm.nih.gov/35226250/
6. Todero, Jenna, Douillet, Christelle, Shumway, Alexandria J, Stýblo, Miroslav, Sethupathy, Praveen. 2023. Molecular and Metabolic Analysis of Arsenic-Exposed Humanized AS3MT Mice. In Environmental health perspectives, 131, 127021. doi:10.1289/EHP12785. https://pubmed.ncbi.nlm.nih.gov/38150313/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen