C57BL/6JCya-Tnfrsf13bem1flox/Cya
Common Name:
Tnfrsf13b-flox
Product ID:
S-CKO-12385
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tnfrsf13b-flox
Strain ID
CKOCMP-57916-Tnfrsf13b-B6J-VA
Gene Name
Product ID
S-CKO-12385
Gene Alias
1200009E08Rik; Taci
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tnfrsf13bem1flox/Cya mice (Catalog S-CKO-12385) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000010286
NCBI RefSeq
NM_021349
Target Region
Exon 4~5
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
Tnfrsf13b, encoding transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), is a B cell-specific tumor necrosis factor (TNF) receptor superfamily member. It drives plasma cell differentiation, regulating various aspects of B cell responses [1,2,3,4].
In antibody deficiency syndromes, both biallelic and monoallelic TNFRSF13B mutations were identified in patients with common variable immunodeficiency disorders. Biallelic mutations were associated with hypogammaglobulinemia and impaired binding to a proliferation-inducing ligand (APRIL). Monoallelic changes, especially the C104R heterozygosity, increased the risk for common variable immunodeficiency disorders, and were associated with low numbers of IgD(-)CD27(+) B cells, benign lymphoproliferation, and autoimmune complications [1]. In addition, mice engineered with A144E variants of tnrsf13B (corresponding to human A181E) showed resistance to Citrobacter rodentium due to natural IgA deficiency in the intestine [2]. In organ transplantation, Tnfrsf13b mutations in mice led to early and severe antibody-mediated rejection (AMR) of cardiac allografts, through decreased "natural" IgM and compromised complement regulation [5].
In conclusion, Tnfrsf13b is crucial for B cell-mediated immune responses. Studies using mouse models have revealed its role in antibody deficiency syndromes, responses to enteric pathogens, and organ transplantation-related immune-mediated pathologies. Understanding Tnfrsf13b's functions provides insights into the mechanisms of these diseases and may inspire novel therapeutic strategies.
References:
1. Salzer, Ulrich, Bacchelli, Chiara, Buckridge, Sylvie, Gaspar, H Bobby, Grimbacher, Bodo. 2008. Relevance of biallelic versus monoallelic TNFRSF13B mutations in distinguishing disease-causing from risk-increasing TNFRSF13B variants in antibody deficiency syndromes. In Blood, 113, 1967-76. doi:10.1182/blood-2008-02-141937. https://pubmed.ncbi.nlm.nih.gov/18981294/
2. Platt, Jeffrey L, de Mattos Barbosa, Mayara Garcia, Huynh, Daniel, Kamada, Nobuhiko, Cascalho, Marilia. 2021. TNFRSF13B polymorphisms counter microbial adaptation to enteric IgA. In JCI insight, 6, . doi:10.1172/jci.insight.148208. https://pubmed.ncbi.nlm.nih.gov/34111031/
3. Cascalho, Marilia, Platt, Jeffrey L. 2022. TNFRSF13B in B cell responses to organ transplantation. In Human immunology, 84, 27-33. doi:10.1016/j.humimm.2022.09.006. https://pubmed.ncbi.nlm.nih.gov/36333165/
4. Cascalho, Marilia, Platt, Jeffrey L. 2021. TNFRSF13B Diversification Fueled by B Cell Responses to Environmental Challenges-A Hypothesis. In Frontiers in immunology, 12, 634544. doi:10.3389/fimmu.2021.634544. https://pubmed.ncbi.nlm.nih.gov/33679786/
5. de Mattos Barbosa, Mayara Garcia, Lefferts, Adam R, Huynh, Daniel, Platt, Jeffrey L, Cascalho, Marilia. 2021. TNFRSF13B genotypes control immune-mediated pathology by regulating the functions of innate B cells. In JCI insight, 6, . doi:10.1172/jci.insight.150483. https://pubmed.ncbi.nlm.nih.gov/34283811/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen