C57BL/6JCya-Hic2em1flox/Cya
Common Name:
Hic2-flox
Product ID:
S-CKO-12391
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Hic2-flox
Strain ID
CKOCMP-58180-Hic2-B6J-VA
Gene Name
Product ID
S-CKO-12391
Gene Alias
HRG22; mKIAA1020
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hic2em1flox/Cya mice (Catalog S-CKO-12391) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000090190
NCBI RefSeq
NM_178922
Target Region
Exon 3
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Hic2, also known as HIC ZBTB transcriptional repressor 2, is a gene involved in multiple biological processes. It plays a crucial role in cardiac development and is associated with the regulation of hemoglobin switching. Genetic models, such as mouse models, are valuable for studying its functions [3,4,5].
In cardiac development, a genetrap mouse allele of Hic2 showed that homozygosity for the allele was embryonic lethal before E10.5, while heterozygous embryos had a partially penetrant late lethality and one-third of them had a cardiac phenotype like ventricular septal defect with over-riding aorta. Conditional targeting indicated its requirement within the Nkx2.5+ and Mesp1+ cardiovascular progenitor lineages [5].
In hemoglobin switching, Hic2 represses BCL11A transcription. Forced expression of Hic2 in adult erythroid cells inhibits BCL11A transcription and induces fetal β-type globin (HBG) expression, while loss of Hic2 in fetal erythroblasts increases BCL11A transcription. Let-7 miRNAs repress Hic2, regulating BCL11A transcription and hemoglobin switching [1,2].
In glioma, Hic2 is hypermethylated and expressed at low levels, predicting poor prognosis. Overexpression of Hic2 inhibits glioma cell proliferation in vitro and in vivo [3].
In conclusion, Hic2 is an important regulator in cardiac development and hemoglobin switching. Mouse models, especially KO/CKO models, have been instrumental in revealing its functions in these processes. In addition, its role in glioma prognosis and growth regulation provides potential implications for glioma treatment.
References:
1. Huang, Peng, Peslak, Scott A, Ren, Ren, Cheng, Xiaodong, Blobel, Gerd A. 2022. HIC2 controls developmental hemoglobin switching by repressing BCL11A transcription. In Nature genetics, 54, 1417-1426. doi:10.1038/s41588-022-01152-6. https://pubmed.ncbi.nlm.nih.gov/35941187/
2. Huang, Peng, Peslak, Scott A, Shehu, Vanessa, Blobel, Gerd A, Khandros, Eugene. . let-7 miRNAs repress HIC2 to regulate BCL11A transcription and hemoglobin switching. In Blood, 143, 1980-1991. doi:10.1182/blood.2023023399. https://pubmed.ncbi.nlm.nih.gov/38364109/
3. Luo, Feifei, Liao, Yifu, Cao, Endong, Zhou, Dong, Cai, Haiping. 2023. Hypermethylation of HIC2 is a potential prognostic biomarker and tumor suppressor of glioma based on bioinformatics analysis and experiments. In CNS neuroscience & therapeutics, 29, 1154-1167. doi:10.1111/cns.14093. https://pubmed.ncbi.nlm.nih.gov/36650953/
4. Dykes, Iain M, van Bueren, Kelly Lammerts, Scambler, Peter J. 2017. HIC2 regulates isoform switching during maturation of the cardiovascular system. In Journal of molecular and cellular cardiology, 114, 29-37. doi:10.1016/j.yjmcc.2017.10.007. https://pubmed.ncbi.nlm.nih.gov/29061339/
5. Dykes, Iain M, van Bueren, Kelly Lammerts, Ashmore, Rebekah J, Bhattacharya, Shoumo, Scambler, Peter J. 2014. HIC2 is a novel dosage-dependent regulator of cardiac development located within the distal 22q11 deletion syndrome region. In Circulation research, 115, 23-31. doi:10.1161/CIRCRESAHA.115.303300. https://pubmed.ncbi.nlm.nih.gov/24748541/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen