C57BL/6JCya-Extl2em1flox/Cya
Common Name:
Extl2-flox
Product ID:
S-CKO-12399
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Extl2-flox
Strain ID
CKOCMP-58193-Extl2-B6J-VA
Gene Name
Product ID
S-CKO-12399
Gene Alias
3000001D04Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Extl2em1flox/Cya mice (Catalog S-CKO-12399) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029575
NCBI RefSeq
NM_021388
Target Region
Exon 3
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
EXTL2, one of the three EXT-like genes in the human genome homologous to EXT1 and EXT2, encodes an N-acetylhexosaminyltransferase. It is involved in glycosaminoglycan (GAG) biosynthesis, a process crucial for various biological functions such as cell-extracellular matrix crosstalk and cell signaling regulation [1,4,5]. Genetic models, like knockout mice, have been valuable in studying its function.
In EXTL2-knockout mice, GAG production was significantly higher than in wild-type mice [1,4,5]. Under CCl4-induced liver failure, hepatocyte proliferation was lower in EXTL2-knockout mice, impairing liver regeneration. This was due in part to reduced hepatocyte-growth-factor-mediated signaling [1,5]. In an induced chronic kidney disease model, matrix mineralization in vascular smooth muscle cells of EXTL2-knockout mice was enhanced, as altered GAG biosynthesis affected bone-morphogenetic-protein signaling and increased the differentiation of VSMCs into osteoblasts [1]. In gastric cancer cell models, KO of EXTL2 enhanced HS levels, promoted a more aggressive phenotype with higher motility and invasion, and impaired Ephrin type-A 4 receptor activation [2]. In a spinal cord demyelinating injury model, EXTL2 -/- mice had excessive CSPG deposition, exacerbated axonal damage, myelin disruption, and increased microglia/macrophages within lesions [3].
In conclusion, EXTL2 plays a key role in regulating GAG biosynthesis. The use of EXTL2 knockout mouse models has revealed its significance in processes related to liver regeneration, aortic calcification, cancer cell behavior, and neuroinflammation following demyelination. These findings contribute to understanding the underlying mechanisms of related diseases and may offer potential therapeutic targets.
References:
1. Nadanaka, Satomi, Kitagawa, Hiroshi. 2013. EXTL2 controls liver regeneration and aortic calcification through xylose kinase-dependent regulation of glycosaminoglycan biosynthesis. In Matrix biology : journal of the International Society for Matrix Biology, 35, 18-24. doi:10.1016/j.matbio.2013.10.010. https://pubmed.ncbi.nlm.nih.gov/24176719/
2. Marques, Catarina, Poças, Juliana, Gomes, Catarina, Vivès, Romain R, Magalhães, Ana. 2022. Glycosyltransferases EXTL2 and EXTL3 cellular balance dictates heparan sulfate biosynthesis and shapes gastric cancer cell motility and invasion. In The Journal of biological chemistry, 298, 102546. doi:10.1016/j.jbc.2022.102546. https://pubmed.ncbi.nlm.nih.gov/36181793/
3. Pu, Annie, Mishra, Manoj K, Dong, Yifei, Sawcer, Stephen, Yong, V Wee. 2020. The glycosyltransferase EXTL2 promotes proteoglycan deposition and injurious neuroinflammation following demyelination. In Journal of neuroinflammation, 17, 220. doi:10.1186/s12974-020-01895-1. https://pubmed.ncbi.nlm.nih.gov/32703234/
4. Nadanaka, Satomi, Zhou, Shaobo, Kagiyama, Shoji, Asano, Masahide, Kitagawa, Hiroshi. 2013. EXTL2, a member of the EXT family of tumor suppressors, controls glycosaminoglycan biosynthesis in a xylose kinase-dependent manner. In The Journal of biological chemistry, 288, 9321-33. doi:10.1074/jbc.M112.416909. https://pubmed.ncbi.nlm.nih.gov/23395820/
5. Nadanaka, Satomi, Kagiyama, Shoji, Kitagawa, Hiroshi. . Roles of EXTL2, a member of the EXT family of tumour suppressors, in liver injury and regeneration processes. In The Biochemical journal, 454, 133-45. doi:10.1042/BJ20130323. https://pubmed.ncbi.nlm.nih.gov/23734945/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen