C57BL/6JCya-Rab37em1flox/Cya
Common Name:
Rab37-flox
Product ID:
S-CKO-12412
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Rab37-flox
Strain ID
CKOCMP-58222-Rab37-B6J-VA
Gene Name
Product ID
S-CKO-12412
Gene Alias
B230331O03Rik; B230354I04Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rab37em1flox/Cya mice (Catalog S-CKO-12412) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000067754
NCBI RefSeq
NM_001163753
Target Region
Exon 3~9
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
Rab37, a member of the RAS oncogene family, is a small GTPase that plays a crucial role in vesicle biogenesis, trafficking, and cargo release [1,2,7,8]. It is involved in multiple biological processes, including autophagy, insulin secretion, and the differentiation and function of various cell types. These functions are essential for maintaining normal physiological homeostasis and are associated with several disease-related pathways. Genetic models, especially gene knockout mouse models, have been valuable in studying Rab37's functions.
Conditional knockout of Rab37 in oocytes impairs autophagy proficiency in the ovary, interfering with follicular homeostasis and ovary development in mice, highlighting its role in maintaining ovarian function [1]. In pancreatic β-cells, the active form of Rab37 is essential for autophagy-promoted insulin secretion, as demonstrated in rab37 knockout cells and mice, emphasizing its importance in glucose homeostasis [2]. In adipose-derived mesenchymal stem cells (ADSCs), Rab37 promotes endothelial differentiation and accelerates ADSC-mediated diabetic wound healing, and in human adipose-derived mesenchymal stem cells (hADSCs), it positively regulates adipogenic differentiation [3,4]. In the context of cancer, in M2 macrophages, Rab37 mediates ST2L trafficking to the plasma membrane, promoting a positive-feedback loop that limits chemotherapeutic efficacy in lung cancer [5]. In T cells, Rab37 mediates trafficking and membrane presentation of PD-1 to sustain T-cell exhaustion in lung cancer [6].
In conclusion, Rab37 is essential for multiple biological processes such as autophagy, cell differentiation, and secretion. The use of KO/CKO mouse models has revealed its significance in diseases related to ovarian function, metabolic regulation, and cancer progression. Understanding Rab37's functions provides insights into the underlying mechanisms of these biological processes and diseases, potentially offering new directions for therapeutic interventions.
References:
1. Xu, Xu, Hu, Mengxin, Ying, Ruhong, Cheng, Hanhua, Zhou, Rongjia. 2024. RAB37-mediated autophagy guards ovarian homeostasis and function. In Autophagy, 20, 2738-2751. doi:10.1080/15548627.2024.2389568. https://pubmed.ncbi.nlm.nih.gov/39113565/
2. Wu, Shan-Ying, Wu, Hung-Tsung, Wang, Yi-Ching, Lan, Sheng-Hui, Liu, Hsiao-Sheng. 2022. Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo. In Autophagy, 19, 1239-1257. doi:10.1080/15548627.2022.2123098. https://pubmed.ncbi.nlm.nih.gov/36109708/
3. Huang, Haili, Liang, Ling, Sun, Dan, Tang, Xudong, Zhang, Peihua. 2023. Rab37 Promotes Endothelial Differentiation and Accelerates ADSC-Mediated Diabetic Wound Healing through Regulating Secretion of Hsp90α and TIMP1. In Stem cell reviews and reports, 19, 1019-1033. doi:10.1007/s12015-022-10491-0. https://pubmed.ncbi.nlm.nih.gov/36627432/
4. Huang, Haili, Li, Anran, Li, Jin, He, Chengzhang, Zhang, Peihua. 2021. RAB37 Promotes Adipogenic Differentiation of hADSCs via the TIMP1/CD63/Integrin Signaling Pathway. In Stem cells international, 2021, 8297063. doi:10.1155/2021/8297063. https://pubmed.ncbi.nlm.nih.gov/34858503/
5. Yang, You-En, Hu, Meng-Hsuan, Zeng, Yen-Chen, Chang, Chih-Peng, Wang, Yi-Ching. 2024. IL-33/NF-κB/ST2L/Rab37 positive-feedback loop promotes M2 macrophage to limit chemotherapeutic efficacy in lung cancer. In Cell death & disease, 15, 356. doi:10.1038/s41419-024-06746-y. https://pubmed.ncbi.nlm.nih.gov/38778059/
6. Kuo, Wan-Ting, Kuo, I-Ying, Hsieh, Hung-Chia, Su, Wu-Chou, Wang, Yi-Ching. 2024. Rab37 mediates trafficking and membrane presentation of PD-1 to sustain T cell exhaustion in lung cancer. In Journal of biomedical science, 31, 20. doi:10.1186/s12929-024-01009-6. https://pubmed.ncbi.nlm.nih.gov/38321486/
7. Wu, Shan-Ying, Chen, Jia-Wen, Liu, Hsi-Yu, Liu, Hsiao-Sheng, Lan, Sheng-Hui. 2022. Secretory autophagy promotes Rab37-mediated exocytosis of tissue inhibitor of metalloproteinase 1. In Journal of biomedical science, 29, 103. doi:10.1186/s12929-022-00886-z. https://pubmed.ncbi.nlm.nih.gov/36457117/
8. Wu, Shan-Ying, Wang, Yi-Ching, Zuchini, Roberto, Liu, Hsiao-Sheng, Lan, Sheng-Hui. 2023. Secretory autophagy-promoted cargo exocytosis requires active RAB37. In Autophagy, 20, 933-934. doi:10.1080/15548627.2023.2210446. https://pubmed.ncbi.nlm.nih.gov/37151129/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen