C57BL/6JCya-Mmp19em1flox/Cya
Common Name:
Mmp19-flox
Product ID:
S-CKO-12413
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mmp19-flox
Strain ID
CKOCMP-58223-Mmp19-B6J-VA
Gene Name
Product ID
S-CKO-12413
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mmp19em1flox/Cya mice (Catalog S-CKO-12413) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026411
NCBI RefSeq
NM_021412
Target Region
Exon 3~6
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Mmp19, a member of the matrix metalloproteinases (MMPs) family, is a zinc-dependent endopeptidase. MMPs play crucial roles in remodeling the extracellular matrix and are involved in various physiological and pathological processes [1,4,5,7]. Mmp19 has been associated with pathways related to cell-matrix interactions, cell migration, and tissue repair [1,3,6].
In animal models, MMP19-deficient mice showed impaired T cell-mediated immune reactions in contact hypersensitivity, with limited inflammatory cell influx, low keratinocyte proliferation, and reduced activated CD8(+) T cells. The development of thymocytes was also altered, suggesting Mmp19 is essential for T cell development and cutaneous immune responses [6]. In pulmonary fibrosis, MMP19 promoted endothelial-to-mesenchymal transition (E(nd)MT), monocyte infiltration, and endothelial barrier permeability. Blocking its interacting proteins SDF1 and ET1 alleviated fibrosis, indicating a potential therapeutic target [1]. In idiopathic pulmonary fibrosis (IPF), MMP19 variants were identified in familial and sporadic cases, with plasma levels higher in patients, providing new clues to IPF pathogenesis [2].
In conclusion, Mmp19 is important for T cell development and cutaneous immune responses. In disease areas, it has a significant impact on pulmonary fibrosis and IPF. Studies using gene-knockout mouse models have revealed its roles in these biological processes and diseases, contributing to a better understanding of related mechanisms and potential therapeutic strategies.
References:
1. Zhao, Weiming, Wang, Lan, Yang, Juntang, Rosas, Ivan, Yu, Guoying. 2023. Endothelial cell-derived MMP19 promotes pulmonary fibrosis by inducing E(nd)MT and monocyte infiltration. In Cell communication and signaling : CCS, 21, 56. doi:10.1186/s12964-023-01040-4. https://pubmed.ncbi.nlm.nih.gov/36915092/
2. Fan, Yali, Zheng, Chunming, Ma, Ruimin, Yang, Shuqiao, Ye, Qiao. 2023. MMP19 Variants in Familial and Sporadic Idiopathic Pulmonary Fibrosis. In Lung, 201, 571-580. doi:10.1007/s00408-023-00652-4. https://pubmed.ncbi.nlm.nih.gov/37971547/
3. Müller, Matthias, Beck, Inken M, Gadesmann, Judith, Reiss, Karina, Sedlacek, Radislav. 2010. MMP19 is upregulated during melanoma progression and increases invasion of melanoma cells. In Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 23, 511-21. doi:10.1038/modpathol.2009.183. https://pubmed.ncbi.nlm.nih.gov/20098411/
4. Craig, Vanessa J, Zhang, Li, Hagood, James S, Owen, Caroline A. . Matrix metalloproteinases as therapeutic targets for idiopathic pulmonary fibrosis. In American journal of respiratory cell and molecular biology, 53, 585-600. doi:10.1165/rcmb.2015-0020TR. https://pubmed.ncbi.nlm.nih.gov/26121236/
5. Chen, Zaiping, Wu, Guiyang, Ye, Fubo, Zhu, Xiongwen, Wu, Chongshan. 2019. High expression of MMP19 is associated with poor prognosis in patients with colorectal cancer. In BMC cancer, 19, 448. doi:10.1186/s12885-019-5673-6. https://pubmed.ncbi.nlm.nih.gov/31088409/
6. Beck, Inken M, Rückert, René, Brandt, Katja, Mentlein, Rolf, Sedlacek, Radislav. 2008. MMP19 is essential for T cell development and T cell-mediated cutaneous immune responses. In PloS one, 3, e2343. doi:10.1371/journal.pone.0002343. https://pubmed.ncbi.nlm.nih.gov/18523579/
7. Wang, Zhuo, Efferth, Thomas, Hua, Xin, Zhang, Xin-An. 2022. Medicinal plants and their secondary metabolites in alleviating knee osteoarthritis: A systematic review. In Phytomedicine : international journal of phytotherapy and phytopharmacology, 105, 154347. doi:10.1016/j.phymed.2022.154347. https://pubmed.ncbi.nlm.nih.gov/35914361/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen