C57BL/6JCya-Nectin1em1flox/Cya
Common Name:
Nectin1-flox
Product ID:
S-CKO-12420
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nectin1-flox
Strain ID
CKOCMP-58235-Nectin1-B6J-VA
Gene Name
Product ID
S-CKO-12420
Gene Alias
Cd111; HIgR; HveC; PRR; PRR1; Pvrl1; nectin-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nectin1em1flox/Cya mice (Catalog S-CKO-12420) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034510
NCBI RefSeq
NM_021424
Target Region
Exon 3~4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Nectin1, also known as CD111 or PVRL1, is a cell adhesion molecule belonging to the immunoglobulin superfamily. It is involved in cell-cell adhesion and has been considered the primary receptor for several alpha herpesviruses [2]. It may also participate in various biological processes such as adherens junction formation and has potential implications in multiple pathways related to development and disease. Genetic models, especially knockout (KO) mouse models, are valuable for studying its functions.
A Nectin1-mutant mouse model with a single amino acid substitution (F129A) showed significantly alleviated disease manifestations, decreased death rate, and viral loading when infected with a highly virulent pseudorabies virus (PRV) strain, indicating that gene modification in Nectin1 can confer PRV resistance [1]. In melanoma, loss-of-function studies showed that NECTIN1 loss stimulates melanoma cell migration in vitro and spreading in vivo in response to decreased IGF1 signaling, establishing it as a major determinant of melanoma dissemination [3]. In neonatally stressed male mice, postnatal suppression or conditional inactivation of Nectin1 in the medial entorhinal cortex (MEC) impaired spatial memory and reproduced stress-induced deficits in MEC-dependent memories and neuronal plasticity in CA1, suggesting its role in stress-related cognitive disorders [4].
In conclusion, Nectin1 is crucial for cell-cell adhesion and plays diverse roles in virus-host interactions, cancer metastasis, and cognitive functions. Studies using KO or mutant mouse models have significantly contributed to understanding its role in diseases such as PRV-related infections, melanoma, and early-life stress-induced memory deficits.
References:
1. Yang, Xiaohui, Yu, Chuanzhao, Zhang, Qiuyan, Wu, Zhenfang, Yang, Huaqiang. 2022. A Nectin1 Mutant Mouse Model Is Resistant to Pseudorabies Virus Infection. In Viruses, 14, . doi:10.3390/v14050874. https://pubmed.ncbi.nlm.nih.gov/35632616/
2. Qi, Shuhui, Sun, Chao, Wang, Jing, Jiang, Zhigang, Yin, Xin. 2024. Identification of NECTIN1 as a novel restriction factor for flavivirus infection. In mBio, 15, e0270824. doi:10.1128/mbio.02708-24. https://pubmed.ncbi.nlm.nih.gov/39570015/
3. Ablain, Julien, Al Mahi, Amira, Rothschild, Harriet, Lian, Christine G, Zon, Leonard I. 2022. Loss of NECTIN1 triggers melanoma dissemination upon local IGF1 depletion. In Nature genetics, 54, 1839-1852. doi:10.1038/s41588-022-01191-z. https://pubmed.ncbi.nlm.nih.gov/36229674/
4. Wu, Chen, Gong, Qian, Xu, Xue, Chen, Wei, Wang, Xiao-Dong. 2022. Disrupted presynaptic nectin1-based neuronal adhesion in the entorhinal-hippocampal circuit contributes to early-life stress-induced memory deficits. In Translational psychiatry, 12, 141. doi:10.1038/s41398-022-01908-y. https://pubmed.ncbi.nlm.nih.gov/35379771/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen