C57BL/6JCya-Cysltr1em1flox/Cya
Common Name:
Cysltr1-flox
Product ID:
S-CKO-12448
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cysltr1-flox
Strain ID
CKOCMP-58861-Cysltr1-B6J-VA
Gene Name
Product ID
S-CKO-12448
Gene Alias
CysLT1R; Cyslt1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cysltr1em1flox/Cya mice (Catalog S-CKO-12448) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113480
NCBI RefSeq
NM_021476
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Cysltr1, also known as Cysteinyl leukotriene receptor 1, is a key receptor through which cysteinyl leukotrienes (CysLTs), a group of eicosanoids, regulate the pathogenesis of various human diseases [6]. It is involved in multiple signaling pathways such as NF-κB, ERK1/2, and may be associated with G protein-coupled signaling cascade [1,5,4]. Cysltr1 is of great biological importance in processes like allergic reactions, cell proliferation, and senescence [5,4]. Genetic models, such as the generation of CysLTR1 L118F mutant mice, have been used to study its functions [6].
In psoriasis, CYSLTR1 is increased in psoriatic skin lesions. CYSLTR1 knockout (KO) mice induced with imiquimod (IMQ) showed improvement in psoriasis-related parameters like PASI scores, serum IL-17A/F levels, and reduced Th1 and Th17 cells in the spleen and skin compared to wild-type mice. This indicates that Cysltr1 plays a role in psoriasis development [1].
In uveal melanoma (UM), CYSLTR1 is upregulated and its pharmacological blockage reduces the growth and metabolic activity of UM cell lines, suggesting its role in UM progression [2].
In diabetes-induced aortic and testicular injury, montelukast, a CysLTR1 antagonist, protects against injury by reducing levels of CysLTR1, TLR4, NF-κB and related inflammatory markers, and stimulating autophagy [3].
In adenoid hypertrophy, CYSLT1 expression is higher in patients compared to controls and is positively associated with disease severity, and may contribute to its progression by activating ERK1/2 [5].
In conclusion, Cysltr1 is crucial in various disease-related biological processes. The use of KO mouse models has revealed its roles in psoriasis, uveal melanoma, diabetes-related complications, and adenoid hypertrophy. These findings help in understanding the disease mechanisms and may provide potential therapeutic targets for these diseases.
References:
1. Zhao, Junpeng, Chen, Yi, Li, Liming, Li, Xinyu, Lu, Qianjin. 2024. CYSLTR1 antagonist inhibits Th17 cell differentiation by regulating the NF-κB signaling for the treatment of psoriasis. In International journal of biological sciences, 20, 2168-2186. doi:10.7150/ijbs.92514. https://pubmed.ncbi.nlm.nih.gov/38617532/
2. García de Alba Graue, Paulina, Abdouh, Mohamed, Goyeneche, Alicia, Burnier, Julia Valdemarin, Burnier, Miguel N. 2024. CYSLTR1 antagonism displays potent anti-tumor effects in uveal melanoma. In Experimental eye research, 248, 110120. doi:10.1016/j.exer.2024.110120. https://pubmed.ncbi.nlm.nih.gov/39389443/
3. Awad, Ahmed M, Elshaer, Sally L, Gangaraju, Rajashekhar, Abdelaziz, Rania R, Nader, Manar A. 2023. CysLTR1 antagonism by montelukast can ameliorate diabetes-induced aortic and testicular inflammation. In International immunopharmacology, 125, 111127. doi:10.1016/j.intimp.2023.111127. https://pubmed.ncbi.nlm.nih.gov/37907048/
4. Huber, Thomas, Horioka-Duplix, Mizuho, Chen, Yuanhuang, Chen, Yu, Sakmar, Thomas P. 2024. The role of signaling pathways mediated by the GPCRs CysLTR1/2 in melanocyte proliferation and senescence. In Science signaling, 17, eadp3967. doi:10.1126/scisignal.adp3967. https://pubmed.ncbi.nlm.nih.gov/39288219/
5. Gao, Wenjie, Li, Jinying, Li, Quanheng, An, Shuhua. 2018. CYSLTR1 promotes adenoid hypertrophy by activating ERK1/2. In Experimental and therapeutic medicine, 16, 966-970. doi:10.3892/etm.2018.6282. https://pubmed.ncbi.nlm.nih.gov/30116346/
6. Hu, Xiaojun, Mao, Junjie, Zhou, Bin, Pang, Pengfei, Shan, Hong. 2019. Generation and phenotype analysis of CysLTR1 L118F mutant mice. In Journal of cellular biochemistry, 121, 2372-2384. doi:10.1002/jcb.29460. https://pubmed.ncbi.nlm.nih.gov/31742746/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen