C57BL/6JCya-Namptem1flox/Cya
Common Name:
Nampt-flox
Product ID:
S-CKO-12486
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Nampt-flox
Strain ID
CKOCMP-59027-Nampt-B6J-VB
Gene Name
Product ID
S-CKO-12486
Gene Alias
1110035O14Rik; NAmPRTase; Pbef; Pbef1; Visfatin
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Namptem1flox/Cya mice (Catalog S-CKO-12486) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020886
NCBI RefSeq
NM_021524
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Nicotinamide phosphoribosyltransferase (NAMPT), also known as visfatin, is a key regulator of the intracellular nicotinamide adenine dinucleotide (NAD) pool. It catalyzes the rate-limiting step in NAD biosynthesis from nicotinamide, influencing the activity of NAD-dependent enzymes and thus regulating cellular metabolism. NAD is an essential coenzyme in cellular redox reactions and a substrate for NAD-dependent enzymes involved in various biological processes, including DNA repair, gene expression, and cellular metabolism [1,3,4,6].
In cancer, NAMPT is often overexpressed in tumour tissues. Inhibition of NAMPT, for example, using the inhibitor FK866, suppresses cancer stem-like cells associated with therapy-induced senescence in ovarian cancer, which may contribute to chemoresistance. This suggests that targeting NAMPT could be a promising anti-cancer strategy to deplete NAD and impair cellular metabolism [2,6]. In experimental necrotizing enterocolitis, NAMPT inhibition by FK866 alleviates intestinal inflammation by regulating macrophage activation, reducing M1 macrophage polarization [5]. Also, in senile osteoporosis, reduction of NAMPT in bone marrow mesenchymal stem cells (BMSCs) promotes lipogenic differentiation and age-related bone loss, while overexpression of Nampt ameliorates senescence-associated phenotypes and promotes osteogenic potential. The NAMPT activator P7C3 is a novel strategy for reducing senile osteoporosis bone loss [7].
In conclusion, NAMPT plays a crucial role in multiple biological processes and diseases. Studies using loss-of-function models, such as NAMPT inhibition in in vivo studies, have revealed its significance in cancer, intestinal inflammation, and senile osteoporosis. These findings suggest that NAMPT could be a potential therapeutic target for these diseases.
References:
1. Garten, Antje, Schuster, Susanne, Penke, Melanie, de Giorgis, Tommaso, Kiess, Wieland. 2015. Physiological and pathophysiological roles of NAMPT and NAD metabolism. In Nature reviews. Endocrinology, 11, 535-46. doi:10.1038/nrendo.2015.117. https://pubmed.ncbi.nlm.nih.gov/26215259/
2. Nacarelli, Timothy, Fukumoto, Takeshi, Zundell, Joseph A, Borowsky, Mark E, Zhang, Rugang. 2019. NAMPT Inhibition Suppresses Cancer Stem-like Cells Associated with Therapy-Induced Senescence in Ovarian Cancer. In Cancer research, 80, 890-900. doi:10.1158/0008-5472.CAN-19-2830. https://pubmed.ncbi.nlm.nih.gov/31857293/
3. Velma, Ganga Reddy, Krider, Isabella S, Alves, Erick T M, Laham, Megan S, Thatcher, Gregory R J. 2024. Channeling Nicotinamide Phosphoribosyltransferase (NAMPT) to Address Life and Death. In Journal of medicinal chemistry, 67, 5999-6026. doi:10.1021/acs.jmedchem.3c02112. https://pubmed.ncbi.nlm.nih.gov/38580317/
4. Ozgencil, Fikriye, Gunindi, Habibe Beyza, Eren, Gokcen. 2024. Dual-targeted NAMPT inhibitors as a progressive strategy for cancer therapy. In Bioorganic chemistry, 149, 107509. doi:10.1016/j.bioorg.2024.107509. https://pubmed.ncbi.nlm.nih.gov/38824699/
5. Liu, Qianyang, Gao, Kai, Ding, Xionghui, Chen, Gongli, Guo, Chunbao. 2023. NAMPT inhibition relieves intestinal inflammation by regulating macrophage activation in experimental necrotizing enterocolitis. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 165, 115012. doi:10.1016/j.biopha.2023.115012. https://pubmed.ncbi.nlm.nih.gov/37329710/
6. Gasparrini, Massimiliano, Giovannuzzi, Simone, Nocentini, Alessio, Raffaelli, Nadia, Supuran, Claudiu T. 2024. Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) in cancer: a patent review. In Expert opinion on therapeutic patents, 34, 565-582. doi:10.1080/13543776.2024.2367006. https://pubmed.ncbi.nlm.nih.gov/38861278/
7. Bai, Chao-Wen, Tian, Bo, Zhang, Ming-Chao, Shan, Hua-Jian, Bai, Jin-Yu. 2024. Targeting NAMPT-OPA1 for treatment of senile osteoporosis. In Aging cell, 24, e14400. doi:10.1111/acel.14400. https://pubmed.ncbi.nlm.nih.gov/39543818/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen