C57BL/6JCya-Gpa33em1flox/Cya
Common Name:
Gpa33-flox
Product ID:
S-CKO-12524
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gpa33-flox
Strain ID
CKOCMP-59290-Gpa33-B6J-VA
Gene Name
Product ID
S-CKO-12524
Gene Alias
2010310L10Rik; 2210401D16Rik; mA33
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpa33em1flox/Cya mice (Catalog S-CKO-12524) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000060833
NCBI RefSeq
NM_021610
Target Region
Exon 3
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Gpa33, the Ig superfamily protein glycoprotein A33, has been implicated in immune dysregulation. It is expressed on fractions of B, dendritic, natural killer, and innate lymphoid cells, with prominent expression in the CD4+ T cell compartment. It may play a role in localization and/or preservation of an undifferentiated state in conventional CD4+ T cells [3].
In the context of Treg cells, GPA33 is expressed on a subset of human Treg cells. It is acquired late during tTreg cell development but not expressed on TGF-β-induced Treg cells. GPA33 can identify Treg cells in human blood that lack the ability to produce effector cytokines, and GPA33high Treg cells can be robustly and stably expanded in vitro, suggesting it can identify human tTreg cells for adoptive cell therapy [1].
In colorectal cancer, GPA33 is a promising surface antigen. Its expression shows intratumoral heterogeneity, and low levels are linked to tumor progression. WNT inhibition can induce its expression, and GPA33-targeted cellular therapy, such as CAR-T cells, can reduce xenograft growth [4]. Moreover, various therapeutic strategies targeting GPA33 in colorectal cancer have been developed, including pretargeted radioimmunotherapy, near-infrared photoimmunotherapy, and bispecific antibody-based therapies [2,5,6,7,8].
In summary, Gpa33 has significant implications in immune cell regulation, especially in identifying stable Treg cells. In colorectal cancer, it serves as an important target for various therapeutic approaches, and understanding its function and regulation through studies like those in the references can potentially lead to more effective treatment strategies for related diseases.
References:
1. Opstelten, Rianne, de Kivit, Sander, Slot, Manon C, Cuadrado, Eloy, Amsen, Derk. 2020. GPA33: A Marker to Identify Stable Human Regulatory T Cells. In Journal of immunology (Baltimore, Md. : 1950), 204, 3139-3148. doi:10.4049/jimmunol.1901250. https://pubmed.ncbi.nlm.nih.gov/32366581/
2. Vaughn, Brett A, Lee, Sang-Gyu, Vargas, Daniela Burnes, Veach, Darren R, Cheal, Sarah M. 2024. Theranostic GPA33-Pretargeted Radioimmunotherapy of Human Colorectal Carcinoma with a Bivalent 177Lu-Labeled Radiohapten. In Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 65, 1611-1618. doi:10.2967/jnumed.124.267685. https://pubmed.ncbi.nlm.nih.gov/39168519/
3. Opstelten, Rianne, Suwandi, Jessica S, Slot, Manon C, Roep, Bart O, Amsen, Derk. 2021. GPA33 is expressed on multiple human blood cell types and distinguishes CD4+ central memory T cells with and without effector function. In European journal of immunology, 51, 1377-1389. doi:10.1002/eji.202048744. https://pubmed.ncbi.nlm.nih.gov/33728639/
4. Börding, Teresa, Janik, Tobias, Bischoff, Philip, Sers, Christine, Horst, David. 2024. GPA33 expression in colorectal cancer can be induced by WNT inhibition and targeted by cellular therapy. In Oncogene, 44, 30-41. doi:10.1038/s41388-024-03200-3. https://pubmed.ncbi.nlm.nih.gov/39472498/
5. Wei, Danfeng, Tao, Ze, Shi, Qiuxiao, Yang, Hao, Jiang, Xian. 2020. Selective Photokilling of Colorectal Tumors by Near-Infrared Photoimmunotherapy with a GPA33-Targeted Single-Chain Antibody Variable Fragment Conjugate. In Molecular pharmaceutics, 17, 2508-2517. doi:10.1021/acs.molpharmaceut.0c00210. https://pubmed.ncbi.nlm.nih.gov/32396000/
6. Wu, Zhihao, Guo, Hong-Fen, Xu, Hong, Cheung, Nai-Kong V. 2018. Development of a Tetravalent Anti-GPA33/Anti-CD3 Bispecific Antibody for Colorectal Cancers. In Molecular cancer therapeutics, 17, 2164-2175. doi:10.1158/1535-7163.MCT-18-0026. https://pubmed.ncbi.nlm.nih.gov/30082472/
7. Chandler, Christopher S, Bell, Meghan M, Chung, Sebastian K, Larson, Steven M, Cheal, Sarah M. 2021. Intraperitoneal Pretargeted Radioimmunotherapy for Colorectal Peritoneal Carcinomatosis. In Molecular cancer therapeutics, 21, 125-137. doi:10.1158/1535-7163.MCT-21-0353. https://pubmed.ncbi.nlm.nih.gov/34667111/
8. Moore, Paul A, Shah, Kalpana, Yang, Yinhua, Bonvini, Ezio, Johnson, Syd. 2018. Development of MGD007, a gpA33 x CD3-Bispecific DART Protein for T-Cell Immunotherapy of Metastatic Colorectal Cancer. In Molecular cancer therapeutics, 17, 1761-1772. doi:10.1158/1535-7163.MCT-17-1086. https://pubmed.ncbi.nlm.nih.gov/29866746/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen