C57BL/6JCya-Wtapem1flox/Cya
Common Name:
Wtap-flox
Product ID:
S-CKO-12549
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Wtap-flox
Strain ID
CKOCMP-60532-Wtap-B6J-VA
Gene Name
Product ID
S-CKO-12549
Gene Alias
2810408K05Rik; 9430038B09Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Wtapem1flox/Cya mice (Catalog S-CKO-12549) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000159986
NCBI RefSeq
NM_001113532
Target Region
Exon 4~5
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
WTAP, short for Wilms tumor 1-associated protein, is a key regulatory subunit of the RNA N6-methyladenosine (m6A) methyltransferase complex. The m6A modification, which WTAP is involved in, regulates multiple biological processes such as RNA splicing, cell proliferation, cell cycle, and embryonic development [2,5,6]. It exerts its m6A modification function by binding to methyltransferase-like 3 (METTL3) in the nucleus to form the METTL3-methyltransferase-like 14 (METTL14)-WTAP (MMW) complex, localizing to nuclear patches [5].
In various disease models, WTAP shows distinct effects. In hepatocellular carcinoma (HCC), WTAP is highly expressed, promoting cell proliferation and tumor growth both in vitro and in vivo. It epigenetically silences ETS1 through m6A-HuR-dependent mechanisms, and modulation of the G2/M phase of HCC cells occurs via a p21/p27-dependent pattern mediated by ETS1 [1]. In diabetic nephropathy, WTAP promotes the m6A methylation of NLRP3 mRNA, upregulating NLRP3 inflammasome activation, cell pyroptosis, and inflammation [3]. Hepatic deletion of Wtap in mice leads to non-alcoholic steatohepatitis (NASH) due to increased lipolysis in white adipose tissue, enhanced hepatic free fatty acids uptake, and induced inflammation, mediated by IGFBP1, CD36, and cytochemokines such as CCL2 respectively [4].
In conclusion, WTAP is crucial in regulating m6A-mediated RNA metabolism, which impacts various biological processes. Its role in disease development, as demonstrated by gene knockout (KO) or conditional knockout (CKO) mouse models in areas like cancer, diabetic nephropathy, and NASH, provides potential therapeutic targets. Understanding WTAP's functions helps in unravelling the molecular mechanisms of these diseases and developing novel treatment strategies.
References:
1. Chen, Yunhao, Peng, Chuanhui, Chen, Junru, Wu, Jian, Zheng, Shusen. 2019. WTAP facilitates progression of hepatocellular carcinoma via m6A-HuR-dependent epigenetic silencing of ETS1. In Molecular cancer, 18, 127. doi:10.1186/s12943-019-1053-8. https://pubmed.ncbi.nlm.nih.gov/31438961/
2. Ping, Xiao-Li, Sun, Bao-Fa, Wang, Lu, Liu, Feng, Yang, Yun-Gui. 2014. Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase. In Cell research, 24, 177-89. doi:10.1038/cr.2014.3. https://pubmed.ncbi.nlm.nih.gov/24407421/
3. Lan, Jianzi, Xu, Bowen, Shi, Xin, Pan, Qi, Tao, Qing. 2022. WTAP-mediated N6-methyladenosine modification of NLRP3 mRNA in kidney injury of diabetic nephropathy. In Cellular & molecular biology letters, 27, 51. doi:10.1186/s11658-022-00350-8. https://pubmed.ncbi.nlm.nih.gov/35761192/
4. Li, Xinzhi, Ding, Kaixin, Li, Xueying, Chen, Xiao-Wei, Chen, Zheng. 2022. Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH). In Nature communications, 13, 4549. doi:10.1038/s41467-022-32163-w. https://pubmed.ncbi.nlm.nih.gov/35927268/
5. Fan, Yongfei, Li, Xinwei, Sun, Huihui, Zhu, Zheng, Yuan, Kai. 2022. Role of WTAP in Cancer: From Mechanisms to the Therapeutic Potential. In Biomolecules, 12, . doi:10.3390/biom12091224. https://pubmed.ncbi.nlm.nih.gov/36139062/
6. Huang, Qibo, Mo, Jie, Liao, Zhibin, Chen, Xiaoping, Zhang, Bixiang. 2022. The RNA m6A writer WTAP in diseases: structure, roles, and mechanisms. In Cell death & disease, 13, 852. doi:10.1038/s41419-022-05268-9. https://pubmed.ncbi.nlm.nih.gov/36207306/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen