C57BL/6JCya-Trp53inp1em1flox/Cya
Common Name:
Trp53inp1-flox
Product ID:
S-CKO-12557
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Trp53inp1-flox
Strain ID
CKOCMP-60599-Trp53inp1-B6J-VA
Gene Name
Product ID
S-CKO-12557
Gene Alias
2700057G22Rik; SIP; SIP18; SIP27; Stinp; Teap; Tp53inp1; p53DINP1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trp53inp1em1flox/Cya mice (Catalog S-CKO-12557) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029865
NCBI RefSeq
NM_021897
Target Region
Exon 2~3
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Trp53inp1, also known as tumor protein 53-induced nuclear protein 1, is a stress-induced protein. It acts as a dual positive regulator of transcription and autophagy, and is involved in antiproliferative and proapoptotic processes. It participates in pathways like the Trp53-Trp53inp1-Tnfrsf10b pathway, which responds to genotoxic damage in spermatogonial stem cells (SSCs) [1]. It is also associated with the p53 pathway and can be regulated by microRNAs like miR-17-92 cluster and miR-125b [3,5].
Trp53inp1-deficient mice showed an increased incidence and multiplicity of colorectal tumors and more severe DSS-induced acute colitis, indicating a role in protecting against oxidative stress-related colitis and colitis-associated cancer [4]. In Trp53inp1 -/- mice, there was additional loss of dopamine neurons in the substantia nigra with ageing and in a Parkinson's disease model, highlighting its neuroprotective role [2]. In the context of radiation-induced SSC apoptosis, Trp53inp1 upregulates Tnfrsf10b upon irradiation, and its deficiency protects SSCs [1].
In summary, Trp53inp1 plays crucial roles in various biological processes including autophagy, apoptosis, and stress response. Its deficiency in mouse models has revealed its significance in diseases such as colorectal cancer, Parkinson's disease, and in the radiation response of SSCs, suggesting it could be a potential target for treatment and prevention of these conditions.
References:
1. Ishii, Kei, Ishiai, Masamichi, Morimoto, Hiroko, Takata, Minoru, Shinohara, Takashi. 2014. The Trp53-Trp53inp1-Tnfrsf10b pathway regulates the radiation response of mouse spermatogonial stem cells. In Stem cell reports, 3, 676-89. doi:10.1016/j.stemcr.2014.08.006. https://pubmed.ncbi.nlm.nih.gov/25358794/
2. Dinh, Emilie, Rival, Thomas, Carrier, Alice, Lortet, Sylviane, Kerkerian-Le Goff, Lydia. 2021. TP53INP1 exerts neuroprotection under ageing and Parkinson's disease-related stress condition. In Cell death & disease, 12, 460. doi:10.1038/s41419-021-03742-4. https://pubmed.ncbi.nlm.nih.gov/33966044/
3. Garg, Neha, Po, Agnese, Miele, Evelina, Ferretti, Elisabetta, Gulino, Alberto. 2013. microRNA-17-92 cluster is a direct Nanog target and controls neural stem cell through Trp53inp1. In The EMBO journal, 32, 2819-32. doi:10.1038/emboj.2013.214. https://pubmed.ncbi.nlm.nih.gov/24076654/
4. Gommeaux, Julien, Cano, Carla, Garcia, Stéphane, Iovanna, Juan, Carrier, Alice. 2007. Colitis and colitis-associated cancer are exacerbated in mice deficient for tumor protein 53-induced nuclear protein 1. In Molecular and cellular biology, 27, 2215-28. doi:. https://pubmed.ncbi.nlm.nih.gov/17242209/
5. Enomoto, Y, Kitaura, J, Hatakeyama, K, Kitamura, T, Sonoki, T. 2011. Eμ/miR-125b transgenic mice develop lethal B-cell malignancies. In Leukemia, 25, 1849-56. doi:10.1038/leu.2011.166. https://pubmed.ncbi.nlm.nih.gov/21738213/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen