C57BL/6JCya-Foxj2em1flox/Cya
Common Name:
Foxj2-flox
Product ID:
S-CKO-12558
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Foxj2-flox
Strain ID
CKOCMP-60611-Foxj2-B6J-VA
Gene Name
Product ID
S-CKO-12558
Gene Alias
Fhx
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Foxj2em1flox/Cya mice (Catalog S-CKO-12558) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003238
NCBI RefSeq
NM_021899
Target Region
Exon 4~6
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
FOXJ2, a member of the Forkhead box transcription factors family, is involved in multiple biological processes. It has been associated with pathways like Notch signaling and is crucial for normal spermatogenesis, cell proliferation, migration, and inflammation regulation [2-6, 9, 10]. Genetic models, such as KO/CKO mouse models, have been instrumental in understanding its functions.
In male mice, FOXJ2 is specifically expressed in meiotic spermatocytes. Using a germ cell-specific conditional knockout model (Foxj2(flox/flox), Mvh-Cre), loss of FOXJ2 led to meiotic arrest and complete infertility, as Foxj2-deficient spermatocytes failed to form chromosomal synapses and perform DSB repair, with reduced expression of related factors [3]. In prostate carcinoma cells, over-expression of FOXJ2 inhibited proliferation, migration, and epithelial-mesenchymal transition through inactivation of the Jagged-1/Notch-1/Hes-1 pathway [2]. In a mouse model of vascular antiphospholipid syndrome (APS), FOXJ2 over-expression, mediated by H3K4me3, accelerated APS pathogenesis by triggering inflammation and thrombosis via the SLAMF8/TREM1 axis [1].
In conclusion, FOXJ2 plays essential roles in spermatogenesis, cancer-related cell behaviors, and inflammatory-thrombotic diseases. Mouse models, especially KO/CKO models, have significantly contributed to understanding its functions in these specific biological processes and disease conditions, providing insights for potential therapeutic strategies in related diseases.
References:
1. Tan, Yuan, Qiao, Jiao, Yang, Shuo, Feng, Weimin, Cui, Liyan. 2024. H3K4me3-Mediated FOXJ2/SLAMF8 Axis Aggravates Thrombosis and Inflammation in β2GPI/Anti-β2GPI-Treated Monocytes. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2309140. doi:10.1002/advs.202309140. https://pubmed.ncbi.nlm.nih.gov/38639399/
2. Guo, Guijun, Zhu, Li. . FOXJ2 Inhibits the Proliferation, Migration and Epithelial-Mesenchymal Transition of Prostate Carcinoma Cells. In Annals of clinical and laboratory science, 52, 731-740. doi:. https://pubmed.ncbi.nlm.nih.gov/36261186/
3. Miao, Hui, Miao, Cong-Xiu, Li, Na, Han, Jing. 2016. FOXJ2 controls meiosis during spermatogenesis in male mice. In Molecular reproduction and development, 83, 684-91. doi:10.1002/mrd.22671. https://pubmed.ncbi.nlm.nih.gov/27316861/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen