C57BL/6NCya-Angptl8em1flox/Cya
Common Name
Angptl8-flox
Product ID
S-CKO-12607
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-624219-Angptl8-B6N-VA
When using this mouse strain in a publication, please cite “Angptl8-flox Mouse (Catalog S-CKO-12607) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Angptl8-flox
Strain ID
CKOCMP-624219-Angptl8-B6N-VA
Gene Name
Product ID
S-CKO-12607
Gene Alias
EG624219, Gm6484, Rifl
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 9
Phenotype
Datasheet
Application
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Strain Description
Ensembl Number
ENSMUST00000058777
NCBI RefSeq
NM_001080940
Target Region
Exon 1~2
Size of Effective Region
~0.8 kb
Overview of Gene Research
ANGPTL8, also known as betatrophin, is an important cytokine and a novel but atypical member of the ANGPTL family. It plays a crucial role in modulating serum glucose and lipid metabolism, especially in regulating lipoprotein lipase (LPL) activity, which is key for fatty acid uptake into tissues [2,3,4,5,6,7,8,9,10]. It has been associated with pathways like NFκB-mediated inflammation and ERK signaling. It is significantly increased in metabolic disorder-related diseases such as type 2 diabetes mellitus (T2DM), obesity, metabolic syndrome, and non-alcoholic fatty liver disease (NAFLD), making it an important target for research into these conditions [3,10]. Gene knockout (KO) mouse models have been valuable in understanding its functions [1].
ANGPTL8 KO mouse studies demonstrated that ANGPTL8 deficiency suppresses high-fat diet (HFD)-stimulated inflammatory activity, hepatic steatosis, and liver fibrosis. The restoration of liver ANGPTL8 expression in KO mice accelerated HFD-induced liver fibrosis. Liver-derived ANGPTL8, as a pro-inflammatory factor, activates hepatic stellate cells (HSCs) by interacting with the LILRB2 receptor to induce ERK signaling and increase the expression of genes promoting liver fibrosis [1].
In conclusion, ANGPTL8 is a key regulator in metabolic homeostasis, with a significant impact on lipid and glucose metabolism. The use of ANGPTL8 KO mouse models has revealed its role in accelerating NAFLD-associated liver fibrosis, highlighting its potential as a diagnostic marker and therapeutic target for liver-related metabolic diseases [1].
References：
1. Zhang, Zongli, Yuan, Yue, Hu, Lin, Ruan, Xuzhi, Guo, Xingrong. 2022. ANGPTL8 accelerates liver fibrosis mediated by HFD-induced inflammatory activity via LILRB2/ERK signaling pathways. In Journal of advanced research, 47, 41-56. doi:10.1016/j.jare.2022.08.006. https://pubmed.ncbi.nlm.nih.gov/36031141/
2. Su, Xin, Cheng, Ye, Wang, Bin. 2021. ANGPTL8 in cardio-metabolic diseases. In Clinica chimica acta; international journal of clinical chemistry, 519, 260-266. doi:10.1016/j.cca.2021.05.017. https://pubmed.ncbi.nlm.nih.gov/34023284/
3. Guo, Chang, Wang, Chenxi, Deng, Xia, Yang, Ling, Yuan, Guoyue. 2021. ANGPTL8 in metabolic homeostasis: more friend than foe? In Open biology, 11, 210106. doi:10.1098/rsob.210106. https://pubmed.ncbi.nlm.nih.gov/34582711/
4. Sylvers-Davie, Kelli L, Davies, Brandon S J. 2021. Regulation of lipoprotein metabolism by ANGPTL3, ANGPTL4, and ANGPTL8. In American journal of physiology. Endocrinology and metabolism, 321, E493-E508. doi:10.1152/ajpendo.00195.2021. https://pubmed.ncbi.nlm.nih.gov/34338039/
5. Luo, Mengdie, Peng, Daoquan. 2018. ANGPTL8: An Important Regulator in Metabolic Disorders. In Frontiers in endocrinology, 9, 169. doi:10.3389/fendo.2018.00169. https://pubmed.ncbi.nlm.nih.gov/29719529/
6. Navaeian, Maryam, Asadian, Samieh, Ahmadpour Yazdi, Hossein, Gheibi, Nematollah. 2021. ANGPTL8 roles in proliferation, metabolic diseases, hypothyroidism, polycystic ovary syndrome, and signaling pathways. In Molecular biology reports, 48, 3719-3731. doi:10.1007/s11033-021-06270-8. https://pubmed.ncbi.nlm.nih.gov/33864588/
7. Abu-Farha, Mohamed, Abubaker, Jehad, Tuomilehto, Jaakko. 2017. ANGPTL8 (betatrophin) role in diabetes and metabolic diseases. In Diabetes/metabolism research and reviews, 33, . doi:10.1002/dmrr.2919. https://pubmed.ncbi.nlm.nih.gov/28722798/
8. Su, Xin, Zhang, Guoming, Cheng, Ye, Wang, Bin. 2021. New insights into ANGPTL8 in modulating the development of cardio-metabolic disorder diseases. In Molecular biology reports, 48, 3761-3771. doi:10.1007/s11033-021-06335-8. https://pubmed.ncbi.nlm.nih.gov/33864591/
9. Abu-Farha, Mohamed, Ghosh, Anindya, Al-Khairi, Irina, Abubaker, Jehad, Prentki, Marc. 2020. The multi-faces of Angptl8 in health and disease: Novel functions beyond lipoprotein lipase modulation. In Progress in lipid research, 80, 101067. doi:10.1016/j.plipres.2020.101067. https://pubmed.ncbi.nlm.nih.gov/33011191/
10. Ye, Huimin, Zong, Qunchuan, Zou, Huajie, Zhang, Ruixia. 2023. Emerging insights into the roles of ANGPTL8 beyond glucose and lipid metabolism. In Frontiers in physiology, 14, 1275485. doi:10.3389/fphys.2023.1275485. https://pubmed.ncbi.nlm.nih.gov/38107478/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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