C57BL/6JCya-Ptgesem1flox/Cya
Common Name:
Ptges-flox
Product ID:
S-CKO-12745
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ptges-flox
Strain ID
CKOCMP-64292-Ptges-B6J-VA
Gene Name
Product ID
S-CKO-12745
Gene Alias
2410099E23Rik; D2Ertd369e; Pges; mPGES; mPGES-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ptgesem1flox/Cya mice (Catalog S-CKO-12745) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102852
NCBI RefSeq
NM_022415
Target Region
Exon 2
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Ptges, prostaglandin E synthase, is an enzyme involved in the arachidonic acid pathway, responsible for the synthesis of prostaglandin E2 (PGE2) [1,2,3,4,5,6,7,8,9]. PGE2 is a key mediator in various physiological and pathological processes, such as inflammation, cell growth, and immune response. Dysregulation of the Ptges-PGE2 axis has been linked to numerous diseases, highlighting its biological importance. Genetic models, especially knockout (KO) mouse models, can offer valuable insights into its functions.
In a Gprc5a-knockout mouse model, Ptges/PGE2 signaling was found to be highly associated with lung tumorigenesis and metastasis. Ptges-knockout in mouse lung tumor cells reduced their stemness and EMT-like features. Although they could still form tumors and lung metastasis in immune-deficient nude mice, they failed to do so in immune-competent mice, suggesting that the major role of Ptges/PGE2 signaling in tumorigenicity and lung metastasis is through immunosuppression [3]. In NSCLC, knockdown of Ptges in lung cancer cells suppressed cell migration, reduced the expression of CSC markers, tumor sphere formation, colony-forming activity, tumorigenicity, and lung metastasis in vivo [5].
In conclusion, Ptges plays a crucial role in the synthesis of PGE2, which is involved in various disease-related processes such as tumorigenesis, metastasis, and immunosuppression. Studies using Ptges KO mouse models have revealed its significance in these disease areas, providing important insights into the underlying mechanisms and potential therapeutic targets.
References:
1. Lin, Min-Hsi, Lee, Yi-Chen, Liao, Jia-Bin, Chou, Chih-Yu, Yang, Yi-Fang. . PTGES is involved in myofibroblast differentiation via HIF-1α-dependent glycolysis pathway. In Journal of cellular and molecular medicine, 28, e70157. doi:10.1111/jcmm.70157. https://pubmed.ncbi.nlm.nih.gov/39417702/
2. Geng, Song, Zhan, Hao, Cao, Lianmeng, Geng, Longlong, Ren, Xiang. 2023. Targeting PTGES/PGE2 axis enhances sensitivity of colorectal cancer cells to 5-fluorouracil. In Biochemistry and cell biology = Biochimie et biologie cellulaire, 101, 501-512. doi:10.1139/bcb-2023-0101. https://pubmed.ncbi.nlm.nih.gov/37358009/
3. Wang, Tong, Jing, Bo, Xu, Dongliang, Zhou, Binhua P, Deng, Jiong. 2020. PTGES/PGE2 signaling links immunosuppression and lung metastasis in Gprc5a-knockout mouse model. In Oncogene, 39, 3179-3194. doi:10.1038/s41388-020-1207-6. https://pubmed.ncbi.nlm.nih.gov/32060421/
4. Murthy, Divya, Attri, Kuldeep S. 2023. PTGES Expression Is Associated with Metabolic and Immune Reprogramming in Pancreatic Ductal Adenocarcinoma. In International journal of molecular sciences, 24, . doi:10.3390/ijms24087304. https://pubmed.ncbi.nlm.nih.gov/37108468/
5. Wang, Tong, Jing, Bo, Sun, Beibei, Zhou, Binhua P, Deng, Jiong. 2019. Stabilization of PTGES by deubiquitinase USP9X promotes metastatic features of lung cancer via PGE2 signaling. In American journal of cancer research, 9, 1145-1160. doi:. https://pubmed.ncbi.nlm.nih.gov/31285948/
6. Orzechowska, Kinga, Kiezun, Marta, Rytelewska, Edyta, Kaminski, Tadeusz, Smolinska, Nina. 2021. Effects of orexin A on PTGS2, PTGES, CBR1 and PGFS mRNA transcript abundances and prostaglandin E2 and F2α concentrations in culture medium of pig uterine explants collected during early gestation and the estrogenic cycle. In Animal reproduction science, 237, 106910. doi:10.1016/j.anireprosci.2021.106910. https://pubmed.ncbi.nlm.nih.gov/34974394/
7. Miyamoto, David K, Flaxman, Hope A, Wu, Hung-Yi, Gao, Jinxu, Woo, Christina M. 2019. Discovery of a Celecoxib Binding Site on Prostaglandin E Synthase (PTGES) with a Cleavable Chelation-Assisted Biotin Probe. In ACS chemical biology, 14, 2527-2532. doi:10.1021/acschembio.9b00511. https://pubmed.ncbi.nlm.nih.gov/31650837/
8. Ma, Qiong, Sun, Jin, Wang, Huan, Gong, Li, Zhang, Wei. 2023. Far upstream element-binding protein 1 confers lobaplatin resistance by transcriptionally activating PTGES and facilitating the arachidonic acid metabolic pathway in osteosarcoma. In MedComm, 4, e257. doi:10.1002/mco2.257. https://pubmed.ncbi.nlm.nih.gov/37180822/
9. Ma, Seongho, Yeom, Jiah, Lim, Young-Hee. 2020. Exogenous NAD+ Stimulates MUC2 Expression in LS 174T Goblet Cells via the PLC-Delta/PTGES/PKC-Delta/ERK/CREB Signaling Pathway. In Biomolecules, 10, . doi:10.3390/biom10040580. https://pubmed.ncbi.nlm.nih.gov/32283838/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen