C57BL/6JCya-Nmiem1flox/Cya
Common Name:
Nmi-flox
Product ID:
S-CKO-12780
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Nmi-flox
Strain ID
CKOCMP-64685-Nmi-B6J-VA
Gene Name
Product ID
S-CKO-12780
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nmiem1flox/Cya mice (Catalog S-CKO-12780) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028314
NCBI RefSeq
NM_019401
Target Region
Exon 4
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Nmi, also known as N-Myc and STAT Interactor protein, is an interferon-inducible protein. It participates in various cellular activities and is involved in multiple signaling pathways, such as NF-κB, MAPK, JAK, TGFβ/Smad, and the regulation of genes like hTERT, IRF7. It is widely associated with processes like tumorigenesis, immune response, and inflammation, thus being of great biological importance. Genetic models, especially gene knockout (KO) mouse models, can significantly aid in studying its functions [1-10].
In a Nmi-knockout mouse model infected with Influenza A virus, the survival rate increased, weight loss decreased, viral replication was lower, and lung inflammation was attenuated compared to wild-type mice. This indicates that Nmi promotes Influenza A virus infection by facilitating the degradation of IRF7 through TRIM21, suppressing the production of type I interferon [3]. In breast cancer, knockdown of Nmi promoted cancer stem cell (CSC) traits, while overexpression inhibited them, with the regulation being at least partially through modulating hTERT signaling [2]. In sepsis, Nmi expression increased in neutrophils and monocytes, and it may exacerbate the inflammatory response through multiple receptors and signaling pathways [1].
In conclusion, Nmi is crucial in processes like viral infection, cancer development, and the inflammatory response in sepsis. Gene knockout mouse models have been instrumental in revealing its roles in these disease-related areas, helping us understand its biological functions and potentially providing new targets for treatment in these diseases.
References:
1. Zeng, Jinhua, Yang, Zixin, Xu, Dan, Qin, Jing, Weng, Zhuangfeng. 2023. NMI Functions as Immuno-regulatory Molecule in Sepsis by Regulating Multiple Signaling Pathways. In Inflammation, 47, 60-73. doi:10.1007/s10753-023-01893-4. https://pubmed.ncbi.nlm.nih.gov/37679586/
2. Feng, Xu, Xu, Xiangdong, Xiao, Xiangsheng, Kang, Lan, Deng, Wuguo. 2017. NMI inhibits cancer stem cell traits by downregulating hTERT in breast cancer. In Cell death & disease, 8, e2783. doi:10.1038/cddis.2017.200. https://pubmed.ncbi.nlm.nih.gov/28492540/
3. Ouyang, Wei, Cen, Mengyuan, Yang, Liping, Xia, Jingyan, Xu, Feng. . NMI Facilitates Influenza A Virus Infection by Promoting Degradation of IRF7 through TRIM21. In American journal of respiratory cell and molecular biology, 65, 30-40. doi:10.1165/rcmb.2020-0391OC. https://pubmed.ncbi.nlm.nih.gov/33761305/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen