C57BL/6JCya-Ap3m2em1flox/Cya
Common Name:
Ap3m2-flox
Product ID:
S-CKO-12792
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ap3m2-flox
Strain ID
CKOCMP-64933-Ap3m2-B6J-VA
Gene Name
Product ID
S-CKO-12792
Gene Alias
5830445E16Rik; AP-3B
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ap3m2em1flox/Cya mice (Catalog S-CKO-12792) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000163739
NCBI RefSeq
NM_001122820
Target Region
Exon 3
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Ap3m2, also known as adaptor-related protein complex 3 subunit mu 2, is involved in the protein trafficking to lysosomes pathway, especially in neuronal cells. It is also essential for normal physiological functions, as its knockout in mice has been used to study various biological processes [2,3].
In colorectal cancer (CRC), AP3M2 is amplified in cell lines. Knockdown of AP3M2 significantly reduces the viability of CRC cell lines HCT-116, CACO2, and HT29, and there is an interaction between its expression and autophagy-related genes, as well as reactive oxygen species (ROS) levels, suggesting targeting it could be a treatment strategy [1].
In epilepsy research, although no missense or nonsense mutations were detected in Japanese epilepsy patients, some AP3M2 mutations may still be candidates for unmapped disorders including epilepsy [2].
In alcohol-related studies, functional validation in Ap3m2 knockout mice confirmed the relationship between Ap3m2 and alcohol preference, withdrawal, and its expression was shown to be genetically and alcohol-induced regulated [3].
In colon cancer, AP3M2 can distinguish between colorectal cancer and adenomas, etc., and higher expression is associated with longer overall survival in colon adenocarcinoma, and it may also predict chemotherapy effectiveness and prognosis [4].
In conclusion, Ap3m2 plays a crucial role in protein trafficking, especially in neurons. Through gene-knockout mouse models, its functions in diseases such as colorectal cancer, epilepsy, and alcohol-related behaviors have been revealed. Understanding Ap3m2's functions contributes to the exploration of potential treatment strategies for these diseases.
References：
1. El Boustani, Maguie, Mouawad, Nayla, Abou Alezz, Monah. 2024. AP3M2: A key regulator from the nervous system modulates autophagy in colorectal cancer. In Tissue & cell, 91, 102593. doi:10.1016/j.tice.2024.102593. https://pubmed.ncbi.nlm.nih.gov/39488930/
2. Huang, Ming-Chih, Okada, Motohiro, Nakatsu, Fubito, Ohno, Hiroshi, Kojima, Toshio. 2007. Mutation screening of AP3M2 in Japanese epilepsy patients. In Brain & development, 29, 462-7. doi:. https://pubmed.ncbi.nlm.nih.gov/17293072/
3. Bubier, Jason A, Jay, Jeremy J, Baker, Christopher L, Crabbe, John C, Chesler, Elissa J. 2014. Identification of a QTL in Mus musculus for alcohol preference, withdrawal, and Ap3m2 expression using integrative functional genomics and precision genetics. In Genetics, 197, 1377-93. doi:10.1534/genetics.114.166165. https://pubmed.ncbi.nlm.nih.gov/24923803/
4. Jin, Qianqian, Feng, Jiahao, Yan, Yang, Kuang, Yong. 2024. Prognostic and immunological role of adaptor related protein complex 3 subunit mu2 in colon cancer. In Scientific reports, 14, 483. doi:10.1038/s41598-023-50452-2. https://pubmed.ncbi.nlm.nih.gov/38177168/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen