C57BL/6JCya-Asb2em1flox/Cya
Common Name
Asb2-flox
Product ID
S-CKO-12815
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-65256-Asb2-B6J-VA
Status
When using this mouse strain in a publication, please cite “Asb2-flox Mouse (Catalog S-CKO-12815) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Asb2-flox
Strain ID
CKOCMP-65256-Asb2-B6J-VA
Gene Name
Product ID
S-CKO-12815
Gene Alias
1110008E15Rik
Background
C57BL/6JCya
Gene Full Name
ankyrin repeat and SOCS box-containing 2
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 12 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000021617
NCBI Transcript ID
NM_023049
Target Region
Exon 4
Size of Effective Region
~1.0 kb
Overview of Gene Research
Asb2, the ankyrin repeat and SOCS box containing 2 gene, forms an E3 ubiquitin ligase complex. It is involved in multiple biological pathways, including the ubiquitin-proteasome system, which is crucial for the selective degradation of proteins. This gene plays an important role in processes like cardiogenesis, hematopoiesis, and immune cell function, and is associated with diseases such as lymphoma and congenital heart disease [1,2,3,5]. Genetic models, especially KO and CKO mouse models, have been valuable in studying Asb2's functions.
In cardiogenesis, Asb2 acts as the E3 ligase of SMAD9, regulating BMP signalling. Knockdown of Asb2 in zebrafish led to a thinned ventricular wall and dilated ventricle, phenotypes rescued by simultaneous Smad9 knockdown [1]. In mice, conditional deletion of Asb2 using Nkx+/Cre.Asb2fl/fl and AHF-Cre.Asb2fl/fl led to embryonic lethality, incomplete heart looping, and double outlet right ventricle (DORV). These defects were related to up-regulation of its target Filamin A (Flna), and concurrent Flna deletion partially rescued the lethality [3].
In NK cells, Asb2 is regulated by AHR, and its knockdown inhibited cell migration. Activation of AHR induced ASB2-dependent filamin A degradation, and reduction of filamin A increased NK cell migration [4]. In leukemia cells, ASB2 targets filamins A and B for proteasomal degradation, and its knockdown delays retinoic acid-induced differentiation [5].
In conclusion, Asb2 is essential for normal development and function in various biological systems. The study of Asb2 KO and CKO mouse models has provided insights into its role in cardiogenesis, immune cell function, and hematopoiesis, with implications for understanding congenital heart disease and leukemia.
References：
1. Min, Kyung-Duk, Asakura, Masanori, Shirai, Manabu, Takashima, Seiji, Kitakaze, Masafumi. 2021. ASB2 is a novel E3 ligase of SMAD9 required for cardiogenesis. In Scientific reports, 11, 23056. doi:10.1038/s41598-021-02390-0. https://pubmed.ncbi.nlm.nih.gov/34845242/
2. Sartori, Giulio, Napoli, Sara, Cascione, Luciano, Thome, Margot, Bertoni, Francesco. 2021. ASB2 is a direct target of FLI1 that sustains NF-κB pathway activation in germinal center-derived diffuse large B-cell lymphoma. In Journal of experimental & clinical cancer research : CR, 40, 357. doi:10.1186/s13046-021-02159-3. https://pubmed.ncbi.nlm.nih.gov/34763718/
3. Yamak, Abir, Hu, Dongjian, Mittal, Nikhil, Ellinor, Patrick T, Domian, Ibrahim J. 2020. Loss of Asb2 Impairs Cardiomyocyte Differentiation and Leads to Congenital Double Outlet Right Ventricle. In iScience, 23, 100959. doi:10.1016/j.isci.2020.100959. https://pubmed.ncbi.nlm.nih.gov/32179481/
4. Shin, June Ho, Moreno-Nieves, Uriel Y, Zhang, Luhua H, Mace, Emily M, Sunwoo, John B. 2021. AHR Regulates NK Cell Migration via ASB2-Mediated Ubiquitination of Filamin A. In Frontiers in immunology, 12, 624284. doi:10.3389/fimmu.2021.624284. https://pubmed.ncbi.nlm.nih.gov/33717133/
5. Heuzé, Mélina L, Lamsoul, Isabelle, Baldassarre, Massimiliano, Calderwood, David A, Lutz, Pierre G. 2008. ASB2 targets filamins A and B to proteasomal degradation. In Blood, 112, 5130-40. doi:10.1182/blood-2007-12-128744. https://pubmed.ncbi.nlm.nih.gov/18799729/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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