C57BL/6NCya-Eif3fem1flox/Cya
Common Name:
Eif3f-flox
Product ID:
S-CKO-12869
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Eif3f-flox
Strain ID
CKOCMP-66085-Eif3f-B6N-VA
Gene Name
Product ID
S-CKO-12869
Gene Alias
0610037M02Rik; Eif3s5
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Eif3fem1flox/Cya mice (Catalog S-CKO-12869) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033342
NCBI RefSeq
NM_025344
Target Region
Exon 2~4
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Eif3f, the f subunit of eukaryotic initiation factor 3, is crucial for mRNA translation initiation as part of the eIF3 complex. It is involved in multiple biological processes, with its dysregulation linked to various diseases [2,3,5].
In skeletal muscle, eIF3f overexpression leads to hypertrophy via the mTORC1 pathway, and it is a target of the E3 ubiquitin ligase MAFbx/atrogin-1 during atrophy [2]. Gene knockout studies in mice showed that homozygous eIF3f null mutants fail to develop and die at an early embryonic stage, while heterozygous mice with partial eIF3f depletion have reduced muscle mass due to decreased protein synthesis rate and MTORC1 pathway inhibition. During hindlimb immobilization, these heterozygous mice display exacerbated muscle atrophy [4].
In colorectal cancer, eIF3f is upregulated, and it enhances the Serine-Glycine-One-Carbon (SGOC) pathway by increasing the expression of phosphoglycerate dehydrogenase (PHGDH) through its deubiquitinating activity [1]. In hepatocellular carcinoma, eIF3f is also upregulated, promoting fatty acid biosynthesis and tumour malignancy by stabilizing long chain acyl CoA synthetase 4 (ACSL4) [3].
In conclusion, eIF3f is essential for embryonic development and muscle homeostasis through regulating protein synthesis. Its dysregulation in cancer, like colorectal and hepatocellular carcinoma, highlights its potential as a therapeutic target. The use of gene knockout mouse models has been instrumental in uncovering these functions and disease associations of eIF3f.
References:
1. Pan, Qihao, Yu, Fenghai, Jin, Huilin, Meng, Xiangqi, Lee, Mong-Hong. 2023. eIF3f Mediates SGOC Pathway Reprogramming by Enhancing Deubiquitinating Activity in Colorectal Cancer. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2300759. doi:10.1002/advs.202300759. https://pubmed.ncbi.nlm.nih.gov/37544925/
2. Sanchez, Anthony M J, Csibi, Alfredo, Raibon, Audrey, Leibovitch, Serge A, Bernardi, Henri. 2013. eIF3f: a central regulator of the antagonism atrophy/hypertrophy in skeletal muscle. In The international journal of biochemistry & cell biology, 45, 2158-62. doi:10.1016/j.biocel.2013.06.001. https://pubmed.ncbi.nlm.nih.gov/23769948/
3. Zhou, Suiqing, Zhang, Liren, You, Yue, Li, Qing, Wang, Xuehao. 2025. eIF3f promotes tumour malignancy by remodelling fatty acid biosynthesis in hepatocellular carcinoma. In Journal of hepatology, , . doi:10.1016/j.jhep.2025.02.045. https://pubmed.ncbi.nlm.nih.gov/40154622/
4. Docquier, Aurélie, Pavlin, Laura, Raibon, Audrey, Candau, Robin, Bernardi, Henri. 2019. eIF3f depletion impedes mouse embryonic development, reduces adult skeletal muscle mass and amplifies muscle loss during disuse. In The Journal of physiology, 597, 3107-3131. doi:10.1113/JP277841. https://pubmed.ncbi.nlm.nih.gov/31026345/
5. Li, Junhong, Yu, Wandong, Ge, Jianchao, Wang, Pengyu, Shi, Guowei. 2020. Targeting eIF3f Suppresses the Growth of Prostate Cancer Cells by Inhibiting Akt Signaling. In OncoTargets and therapy, 13, 3739-3750. doi:10.2147/OTT.S244345. https://pubmed.ncbi.nlm.nih.gov/32440143/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen