C57BL/6JCya-Fkbp11em1flox/Cya
Common Name:
Fkbp11-flox
Product ID:
S-CKO-12890
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Fkbp11-flox
Strain ID
CKOCMP-66120-Fkbp11-B6J-VA
Gene Name
Product ID
S-CKO-12890
Gene Alias
1110002O23Rik; FKBP-11
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fkbp11em1flox/Cya mice (Catalog S-CKO-12890) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003445
NCBI RefSeq
NM_024169
Target Region
Exon 1~5
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
FKBP11, also known as FK506-binding protein 11, is a member of the peptidyl-prolyl cis-trans isomerase family. It is involved in the unfolded protein response (UPR) and acts as a secretory translocon accessory factor, binding to ribosome-translocon complexes and acting on soluble secretory and transmembrane proteins during their synthesis at the endoplasmic reticulum (ER) [6]. It is transcriptionally regulated by XBP1s and is associated with important cellular processes such as cell growth through activation of the mechanistic target of rapamycin (mTOR) signaling [7].
In obese mice, restoring hepatic FKBP11 expression initiates an atypical UPR signaling pathway. This pathway rewires PERK signaling toward NRF2, away from the eIF2α-ATF4 axis of the UPR, establishing glucose homeostasis without changing hepatic ER stress, food consumption, or body weight, which may provide new treatment avenues for type 2 diabetes and obesity [1]. In hepatocellular carcinoma (HCC), FKBP11 is highly expressed. Knockdown of FKBP11 in HCC cells attenuates proliferation and migration, suggesting its role in HCC development and its potential as a diagnostic and prognostic marker [2]. In osteosarcoma, FKBP11 is highly expressed, and silencing its expression suppresses cell invasion, migration, and proliferation, while promoting apoptosis, and it may act through the MAPK pathway [3]. In oral squamous cell carcinoma (OSCC), FKBP11 promotes cell proliferation by regulating the G2/M phase and apoptosis via p53-related pathways [4]. In Crohn's disease, FKBP11 protects intestinal epithelial cells against inflammation-induced apoptosis by inhibiting the ER stress-associated JNK/caspase apoptotic pathway [5].
In conclusion, FKBP11 plays diverse roles in multiple biological processes and disease conditions. Studies using gene-knockout or knockdown models in various diseases like type 2 diabetes, obesity, different types of cancers, and Crohn's disease have revealed its functions in regulating UPR signaling, cell proliferation, migration, apoptosis, and inflammation. These findings contribute to understanding disease mechanisms and may guide the development of novel therapeutic strategies.
References:
1. Herrema, Hilde, Guan, Dongxian, Choi, Jae Won, Chun, Hyonho, Ozcan, Umut. . FKBP11 rewires UPR signaling to promote glucose homeostasis in type 2 diabetes and obesity. In Cell metabolism, 34, 1004-1022.e8. doi:10.1016/j.cmet.2022.06.007. https://pubmed.ncbi.nlm.nih.gov/35793654/
2. Thokerunga, Erick, Huang, Fangfang, Bongolo, Christian Cedric, Akankwatsa, Gilbert, Tu, Jian-Cheng. . FKBP11 upregulation promotes proliferation and migration in hepatocellular carcinoma. In Cancer biomarkers : section A of Disease markers, 37, 109-120. doi:10.3233/CBM-220440. https://pubmed.ncbi.nlm.nih.gov/37248890/
3. Zeng, Duo, Li, Jiayu, Yuan, Xuhui, Lv, Xiao-Bin, Liao, Qi. 2023. FKBP11 improves the malignant property of osteosarcoma cells and acts as a prognostic factor of osteosarcoma. In Aging, 15, 2450-2459. doi:10.18632/aging.204523. https://pubmed.ncbi.nlm.nih.gov/37014329/
4. Qiu, Lin, Liu, Han, Wang, Shuang, Wang, Guan-Hua, Zhang, Jun. 2021. FKBP11 promotes cell proliferation and tumorigenesis via p53-related pathways in oral squamous cell carcinoma. In Biochemical and biophysical research communications, 559, 183-190. doi:10.1016/j.bbrc.2021.04.096. https://pubmed.ncbi.nlm.nih.gov/33945996/
5. Wang, Xiaotong, Cui, Xiaopeng, Zhu, Chuanwu, Zhang, Dongmei, Zhou, Guoxiong. 2018. FKBP11 protects intestinal epithelial cells against inflammation‑induced apoptosis via the JNK‑caspase pathway in Crohn's disease. In Molecular medicine reports, 18, 4428-4438. doi:10.3892/mmr.2018.9485. https://pubmed.ncbi.nlm.nih.gov/30221722/
6. DiGuilio, Amanda, Cheng, Ben, Zhong, Frank, Coon, Joshua J, Keenan, Robert J. 2024. The prolyl isomerase FKBP11 is a secretory translocon accessory factor. In Molecular biology of the cell, 35, ar135. doi:10.1091/mbc.E24-07-0305. https://pubmed.ncbi.nlm.nih.gov/39259761/
7. Wang, Xiaoding, Deng, Yingfeng, Zhang, Guangyu, Jiang, Xuejun, Wang, Zhao V. 2019. Spliced X-box Binding Protein 1 Stimulates Adaptive Growth Through Activation of mTOR. In Circulation, 140, 566-579. doi:10.1161/CIRCULATIONAHA.118.038924. https://pubmed.ncbi.nlm.nih.gov/31177839/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen